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標題: PMMA-based capillary electrophoresis electrochemical detection microchip fabrication
作者: Horng, R.H.
Han, P.
Chen, H.Y.
Lin, K.W.
Tsai, T.M.
Zen, J.M.
韓 斌
關鍵字: chip;su-8
Project: Journal of Micromechanics and Microengineering
期刊/報告no:: Journal of Micromechanics and Microengineering, Volume 15, Issue 1, Page(s) 6-10.
yIn this paper, a 50 mum (depth) x 50 mum (width) microfluidic channel is made on a poly(methyl methacrylate) (PMMA) substrate using thick photoresist. Openings were drilled for buffer reservoirs on an additional piece of PMMA. A final PMMA/pattemed photoresist/PMMA sandwich configuration was completed using a bonding process. The thick photoresist was used as the adhesion layer and also as the microfluidic system. Using screen-printed technology for carbon and silver electrode fabrication, the microchip electrophoretic device functions were demonstrated. Successful detection of uric acid and L-ascorbic acid (the main components in human urine) validates the functionality of the proposed system. Successful ascorbic and uric acid separation in a sample from a urine donor who had consumed 500 mg of vitamins verified the proposed biochip.
ISSN: 0960-1317
DOI: 10.1088/0960-1317/15/1/002
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