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標題: Evaluation of Subchronic Toxicity of Pet Food Contaminated with Melamine and Cyanuric Acid in Rats
作者: Chen, K.C.
Liao, C.W.
Cheng, F.P.
Chou, C.C.
Chang, S.C.
Wu, J.H.
Zen, J.M.
Chen, Y.T.
Liao, J.W.
關鍵字: subchronic toxicity;contaminated pet food;melamine;cyanuric acid;rats;renal-failure;3 dogs;cats;nephrotoxicity;toxicology
Project: Toxicologic Pathology
期刊/報告no:: Toxicologic Pathology, Volume 37, Issue 7, Page(s) 959-968.
Outbreaks of food-associated renal failure in pets occurred in Asia and the United States of America in 2004 and 2007. They were related to the combined intoxication of cyanuric acid and melamine. Our aims were to investigate cyanuric acid and melamine contamination of pet food and to examine subchronic toxicity in rats. Levels of 10%, 20%, 50%, and 50%-100% (w/w) of contaminated pet food were fed to rats for three months. Analytical results revealed that the tainted food contained significant levels of cyanuric acid and melamine in a ratio of 1:6.8. Rats fed the diet of 50%-100% for three months exhibited elevated serum blood urea nitrogen and creatinine, as well as dose-dependent melamine/cyanuric acid crystal-induced nephrotoxicity. The melamine/cyanuric acid crystals of various sizes were mixed with necrotic cell debris and inflammatory cells, accompanied by tubular dilation and interstitial fibrosis. The immunohistochemistry index of proliferative cellular nuclear antigen and osteopontin in the kidney of the 50%-100% group were elevated, indicating regeneration of renal cells and the formation of crystals. In conclusion, the combination ratio of cyanuric acid to melamine and the acidic urine content were two factors that, upon repeated exposure, determined the severity of the nephrotoxicity.
ISSN: 0192-6233
DOI: 10.1177/0192623309347910
Appears in Collections:化學系所

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