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標題: Fast differentiation of meats from fifteen animal species by liquid chromatography with electrochemical detection using copper nanoparticle plated electrodes
作者: Chou, C.C.
Lin, S.P.
Lee, K.M.
Hsu, C.T.
Vickroy, T.W.
Zen, J.M.
關鍵字: meat differentiation;protein degradation;HPLC-EC;copper nanoparticle;electrode;adulteration;length-polymorphism analysis;capillary-electrophoresis;mitochondrial-dna;gc-ms;identification;feed;dipeptides;mixtures;products;tissues
Project: Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
期刊/報告no:: Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, Volume 846, Issue 1-2, Page(s) 230-239.
A simple, rapid and reliable method based on high-performance liquid chromatography with electrochemical detection was developed to routinely differentiate among meat products from fifteen food animal species. Samples from cattle, pigs, goats, deer, horses, chickens, ducks, ostriches, salmon, cod, shrimp, crabs, scallops, bullfrogs and alligators each exhibited unique electrochemical profiles. Species-specific markers exhibited reproducible peak retention times with coefficients of variation less then 6% across different runs, body regions and subjects. The method requires no derivatization or extraction steps and may be applicable to fresh or cooked meats. Incubation of fresh beef, pork or chicken at room temperature for 24 h or repeated freezing and thawing changed the intensity but not the pattern of species-specific peaks. In conclusion, this method appears suitable for rapid differentiation of meats from various food animal species and demonstrates the utility of electrochemical detection to supplement existing immunochemical and molecular biological methods. The possibility of using this method to detect adulteration and degradative changes of meat proteins is discussed. (c) 2006 Elsevier B.V. All rights reserved.
ISSN: 1570-0232
DOI: 10.1016/j.jchromb.2006.09.006
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