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標題: Simultaneous detection of NADH and H2O2 using flow injection analysis based on a bifunctional poly(thionine)-modified electrode
作者: Baskar, Selvaraj
Chang, Jen-Lin
Zen, Jyh-Myng
關鍵字: Poly(thionine);Preanodized screen printed carbon;electrode;NADH;Hydrogen peroxide
Project: Biosensors and Bioelectronics, Volume 33, page(s) 95– 99.
We report here a novel detection scheme for simultaneous detection of NADH and H2O2 based on a
bifunctional poly(thionine)-modified electrode. Electropolymerization of thionine on a “preanodized”
screen-printed carbon electrode effectively lowers the oxidation potential of NADH to 0.15 V (vs. Ag/AgCl).
Since poly(thionine) is also a well known electrochemical mediator for H2O2 reduction, we further
developed a poly(thionine)-modified ring disk electrode for simultaneous measurement of nicotinamide
adenine dinucleotide (NADH) and hydrogen peroxide (H2O2) by flow injection analysis. By applying the
optimized detection potentials of 0.2 V and
−0.2 V at disk and ring electrodes, respectively, this system
allows the simultaneous measurement of both analytes with good sensitivity (0.13 A/mM for H2O2 and
0.34 A/mM for NADH) and limit of detection (1.74 M and 26.0 M for NADH and H2O2). This opens
the possibility of a whole series of biosensor applications.
ISSN: 0956-5663
DOI: 10.1016/j.bios.2011.12.025
Appears in Collections:化學系所

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