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標題: 條狀組織培養苗分株扦插系統之研究
Study on the Cutting and Transplanting System for Tubular Tissue Culture Plantlets
作者: 張立偉
關鍵字: Tissue culture;組織培養;Anoectochilus;Tubular crop;Robot;Cuttage;金線蓮;條狀作物;機器人;扦插
出版社: 農業機械工程學系
The multiplication in the traditional tissue culture not only consumes labors and time, but also leads to the devastating outcome, and the death of plantlets caused by pollution. Therefore, the objectives of this research are to develop an experimental model of the cutting and transplanting system for Anoectochilus tissue culture plantlets, and to contribute to the basic development of the automated production for tubular tissue culture plantlets, thus reduce the labor demand and probability of pollution.
The plantlets transplantation system is part of the cutting and transplanting system for Anoectochilus tissue culture plantlets. Within the system, the facilities are comprised of three parts, including plantlets transplantation robot, cutting part and suppression part. The system performs plantlets heave, extraction, cutting, throw away the waste material, put plantlets, suppression and among others. The shortest time taken for the operation of the plantlets transplanting system to complete a cycle which complete the separation and cuttage of a bottle of Anoectochilus plantlets into three new vessels was 2 minutes and 31.6 seconds. The result also indicated that the successful rate of cuttage varied from the position of plantlets, and they were 39.0%, 81.6%, and 100% for the upper region, the middle region, and the lower region, respectively. The overall successful rate was 75.9%. However, including pre- and post-operation procedures such as opening and closing of the bottle, vessel transport and sterilization, the total time consumed for the whole system to run through were 11 minutes and 25 seconds and 10 minutes and 20 seconds for the starting and the normal run, respectively. As revealed by these data, the automatic producing system was able to complete 127 bottles for working eight hours per day, slightly faster than those produced by workers, 110 bottles/day. Therefore, it is possible to replace the traditional hand-made procedures by using automatic system.

Appears in Collections:生物產業機電工程學系

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