Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/35353
標題: 條狀組織培養苗分株扦插系統之研究
Study on the Cutting and Transplanting System for Tubular Tissue Culture Plantlets
作者: 張立偉
關鍵字: Tissue culture;組織培養;Anoectochilus;Tubular crop;Robot;Cuttage;金線蓮;條狀作物;機器人;扦插
出版社: 農業機械工程學系
摘要: 
The multiplication in the traditional tissue culture not only consumes labors and time, but also leads to the devastating outcome, and the death of plantlets caused by pollution. Therefore, the objectives of this research are to develop an experimental model of the cutting and transplanting system for Anoectochilus tissue culture plantlets, and to contribute to the basic development of the automated production for tubular tissue culture plantlets, thus reduce the labor demand and probability of pollution.
The plantlets transplantation system is part of the cutting and transplanting system for Anoectochilus tissue culture plantlets. Within the system, the facilities are comprised of three parts, including plantlets transplantation robot, cutting part and suppression part. The system performs plantlets heave, extraction, cutting, throw away the waste material, put plantlets, suppression and among others. The shortest time taken for the operation of the plantlets transplanting system to complete a cycle which complete the separation and cuttage of a bottle of Anoectochilus plantlets into three new vessels was 2 minutes and 31.6 seconds. The result also indicated that the successful rate of cuttage varied from the position of plantlets, and they were 39.0%, 81.6%, and 100% for the upper region, the middle region, and the lower region, respectively. The overall successful rate was 75.9%. However, including pre- and post-operation procedures such as opening and closing of the bottle, vessel transport and sterilization, the total time consumed for the whole system to run through were 11 minutes and 25 seconds and 10 minutes and 20 seconds for the starting and the normal run, respectively. As revealed by these data, the automatic producing system was able to complete 127 bottles for working eight hours per day, slightly faster than those produced by workers, 110 bottles/day. Therefore, it is possible to replace the traditional hand-made procedures by using automatic system.

傳統組織培養在增殖過程中不但耗費人力與時間、枯燥乏味且易使苗體受到病菌污染而死亡。因此,本研究之目的即發展一套金線蓮組織培養苗分株扦插系統之試驗模型,期能建立條狀苗組織培養生產自動化之基礎,以降低此一產業之人工需求與污染機率。
本苗株移植系統乃是金線蓮組織培養苗生產自動化系統中之一部分。在硬體上包括苗株移植機器人、分株機構及鎮壓機構三大部分,執行包含提升苗株、取苗、分株、丟棄廢棄截株、放苗及鎮壓等動作。苗株移植機器人性能測試結果顯示,一次苗株移植作業即將一瓶金線蓮半成苗分株扦插至三瓶新培養瓶中之最快速度約需費時2分31.6秒;扦插成功率依截株位置之不同而有些差異,上段為39.0%,中段為81.6%,下段為100%,平均扦插成功率為75.9%;整體作業即包括苗瓶之移送、開關瓶及消毒與苗株之分株扦插等動作之時間測試結果顯示,起始循環作業約需費時11分25秒,正常循環作業起約需費時10分20秒,若以一天工作8小時計算,本自動化生產系統約可完成127瓶,略快過於以人工作業之110瓶,因此以機器自動化生產取代傳統人力作業將是可行的方向。
URI: http://hdl.handle.net/11455/35353
Appears in Collections:生物產業機電工程學系

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