Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3577
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dc.contributor李訓清zh_TW
dc.contributor楊錫杭zh_TW
dc.contributor易逸波zh_TW
dc.contributor.advisor劉永銓zh_TW
dc.contributor.author陳俊瑩zh_TW
dc.contributor.authorChen, Jung Yingen_US
dc.contributor.other中興大學zh_TW
dc.date2007zh_TW
dc.date.accessioned2014-06-06T05:32:12Z-
dc.date.available2014-06-06T05:32:12Z-
dc.identifierU0005-2008200615264500zh_TW
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Jensen, and Robert Langer,, Reactive Polymer Coatings: A First Step toward Surface Engineering of Microfluidic Devices. Analytical. Chemistry., 2003. 75: p. 2117-2122. 46. David C. Duffy, J.C.M., Olivier J. A. Schueller, and George M. Whitesides, Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane). Analytical. Chemistry., 1998. 70: p. 4974-4984. 47. 劉博文, ULSI製程技術, 2003: p. 75. 48. 黃振翁, 應用多功能基薄膜純化及固定化盤尼西林醯胺酵素. 2004. 49. 張簡志強, 應用金屬親和薄膜分離純化盤尼西林醯胺酵素. 國立中興大學化學工程學系碩士論文, 2003.zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/3577-
dc.description.abstractPolydimethylsiloxane (PDMS)容易加工以及製作成本低廉,因此廣泛應用在製作微流體晶片上;但PDMS表面呈現疏水性質,所以很難把蛋白質固定在PDMS表面。本研究為了解決上述的問題,用Piranha solution氧化PDMS表面,再加入epichlorohydrin (EPI)反應,使PDMS表面具有環氧基。並以聚離氨酸(84 KDa)以及酪蛋白當介質,進行蛋白質固定於PDMS表面上之研究。結果顯示以酪蛋白為介質時,固定在PDMS表面的蛋白質為較多。將anti-HSA固定在PDMS表面上(此PDMS表面有酪蛋白當介質),運用在競爭免疫分析,線性範圍在0.200~0.800mg/ml,偵測極限為0.031mg/ml。zh_TW
dc.description.abstractPolydimethylsiloxane (PDMS) is widely used in microfluidic chip, because of its ease of fabrication and low cost. PDMS is due to hydrophobic not ease to immobilize protein on PDMS surface. In order to solve the problem, PDMS surface is oxidized with Piranha solution and then epichlorohydrin (EPI) was added. After that, epoxy group was generated on PDMS surface.Polylysine (84 KDa) or casein is intermediate, and protein immobilizes on PDMS surface. While casein is used as the intermediate, the amount of protein immobilized on PDMS surface is larger. Anti-HSA immobilizes on PDMS surface and uses it in competitive immunoassay. The linear range is 0.200~0.800mg/ml with the detection limit of 0.031mg/ml.en_US
dc.description.tableofcontents致 謝 I 摘 要 II ABSTRACT III 目 錄 IV 圖 目 錄 VII 表目錄 XI 第一章 序論 1 第二章文獻回顧 2 2-1、免疫分析 2 2-2、螢光介紹 7 2-3、生物晶片 10 2-4、PDMS改質 13 2-4-1、PDMS本體的改質 14 2-4-2、PDMS的表面改質 17 第三章、實驗材料與方法 29 3-1、實驗材料 29 3-1-1、微流道製作設備 29 3-1-2、螢光偵測設備 29 3-1-3、其他設備 29 3-1-4、實驗藥品 30 3-2、HSA 染色 31 3-2-1、ICT測試 31 3-2-2、HSA-FITC溶液之線性範圍探討 31 3-2-3、螢光溶液之儲存試驗 31 3-2-4、螢光溶液耐光試驗 32 3-2-5、螢光顯微鏡之精密度探討 32 3-3、微流體晶片製作 33 3-3-1、模板的製作 33 3-3-2、翻模製微流道 33 3-3-3、微流體晶片封裝 34 3-4、微流體晶片運用在免疫檢測上 35 3-5、PDMS的表面改質 36 3-5-1、PDMS平板的製作 36 3-5-2、PDMS表面氧化與表面官能基化 36 3-6、將蛋白質固定在PDMS上 38 3-6-1、蛋白質直接共價鍵鍵結固定 38 3-6-2、蛋白質間接共價鍵鍵結固定 38 3-7、蛋白質固定化法之運用在免疫檢測上 39 第四章、結果與討論 40 4-1、HSA染色 40 4-2、微流體晶片製作 45 4-3、微流體晶片運用在免疫檢測上 48 4-4、PDMS的表面改質 50 4-4-1、PDMS表面官能基化 50 4-4-2、PDMS表面反應性之探討 54 4-5、蛋白質共價固定於PDMS表面 57 4-6、蛋白質固定化法之應用 62 4-6-1、定性分析 62 4-6-2、定量分析 64 第五章、結論與展望 65 5-1、結論 65 5-2、未來展望 66 參考文獻 67zh_TW
dc.language.isoen_USzh_TW
dc.publisher化學工程學系所zh_TW
dc.relation.urihttp://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2008200615264500en_US
dc.subjectmicrofluidic chipen_US
dc.subject微流體晶片zh_TW
dc.subjectPDMS (polydimethylsiloxane)en_US
dc.subjectsurface modificationen_US
dc.subjectprotein immobilizationen_US
dc.subjectPDMS (polydimethylsiloxane)zh_TW
dc.subject表面改質zh_TW
dc.subject蛋白質固定化zh_TW
dc.titlePolydimethylsiloxane之表面改質及在免疫分析上之應用zh_TW
dc.titleModification of polydimethylsiloxane and its application in immunoassay.en_US
dc.typeThesis and Dissertationzh_TW
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.openairetypeThesis and Dissertation-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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