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標題: 奈米碳管與膠體金於電化學生物感測器上之應用
Application of Carbon Nanotube and colloid gold to electrochemical biosensor
作者: 陳燕惠
Chen, Yen-Hui
關鍵字: Multi-walls carbon nanotubes;多壁奈米碳管;Colloid gold;Glucose oxidase;Electrochemical biosensor;Protein immobilization;膠體金;葡萄糖氧化酵素;電化學式生物感測器;蛋白質固定化
出版社: 化學工程學系所
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在葡萄糖檢測中探討其最適條件,所得結果如下:葡萄糖氧化酵素(GOD) 濃度為1mg/ml、金溶液添加量為20μl、反應溶液攪拌速度為300rpm、反應溶液pH值為7時,膠體金顆粒/多壁奈米碳管/GOD/ Nafion薄膜修飾玻璃碳電極的偵測靈敏度為0.0043mM,應答時間平均約為8秒,偵測範圍為0.0043 ~ 10.07mM,線性範圍為2.07~10.07mM。

Preparations of biosensors based on the MWCNT/Nafion/glucose oxidase (GOD) coated on the thin film glassy carbon electrode (GCE) for detecting the glucose and hydrogen peroxide (H2O2) were investigated. Different methods such as electrochemical reduction, electrochemical deposition, physical adsorption, blending& prereduction(BP)were used to immobilize the gold nanoparticles onto the MWCNT/Nafion of GCE biosensor (Au/MWCNTs/Nafion/GCE), which could be applied in detection of hydrogen peroxide and glucose concentrations.
With the operating votage of -0.44V, it showed the highest current of 8.37μA with the accumulation of 1.07mM H2O2 was observed when using BP approach. In this study, BP approach could give the highest current, therefore it could be further applied to the detection of glucose concentrations. Under the optimal conditions of 1mg/ml GOD, 20μl gold solution, pH7 and agitation speed of 300rpm, the sensitivity and response time of Au/GOD/MWCNT/Nafion/GCE biosensor were 0.0043 mM and 8 seconds respectively in the detecting range of 0.0043 ~ 10.07mM glucose.
其他識別: U0005-2408200614240500
Appears in Collections:化學工程學系所

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