Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3593
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dc.contributor吳宗明zh_TW
dc.contributorTzong-Ming Wuen_US
dc.contributor廖建勛zh_TW
dc.contributorChien-Shiun Liaoen_US
dc.contributor.advisor蔡毓楨zh_TW
dc.contributor.advisorYu-Chen Tsaien_US
dc.contributor.author廖宏偉zh_TW
dc.contributor.authorLiaw, Hong-Weien_US
dc.contributor.other中興大學zh_TW
dc.date2007zh_TW
dc.date.accessioned2014-06-06T05:32:14Z-
dc.date.available2014-06-06T05:32:14Z-
dc.identifierU0005-2806200616071300zh_TW
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dc.identifier.urihttp://hdl.handle.net/11455/3593-
dc.description.abstract本論文成功研究製備出以多層壁奈米碳管/全氟磺酸聚合物/酒精去氫酶(MWNTs/Nafion/ADH)以及MWNTs/Nafion兩種不同奈米複合材料薄膜修飾玻碳電極(GCE),證明具有偵測酒精、尼古丁的能力,並且成功的運用於真實樣品測量上。本論文第一部份以利用最適當的MWNTs/Nafion/ADH比例值,製備出修飾生物感測器電極,其中MWNTs具有優勢的電化學催化活性特性並且成為奈米導線複合材料,Nafion高分子薄膜則為電化學改良聚合黏結劑,而ADH則為一生物性觸媒,能以較低的活化能促進乙醇轉化成乙醛,還原型菸鹼醯胺腺嘌呤二核苷酸(NADH)為經由酒精氧化反應所產生的產物,MWNTs/Nafion薄膜所製備之電極對於NADH偵測,顯示出奈米碳管修飾薄膜具有能比Nafion修飾薄膜以更低的活化能氧化NADH。奈米碳管為圓管狀,並且其外圍直徑為60 nm,其長度可達數個μm。利用AFM觀察其表面形貌,進而了解ADH在MWNTs/Nafion薄膜中扮演的角色,並且得知ADH能吸附於奈米碳管之上,ADH均勻的分散在薄膜表面也可由AFM圖中所得。MWNTs/Nafion/ADH奈米複合材料修飾電極對於酒精化學物質,其奈米碳管展現出優勢的偵測表現是由循環伏安法及安培法所證明,由不同添加量的ADH對於酒精偵測之效能,選擇0.7 V的最適操作電位,得知添加6 mg/mL ADH具有較佳效能,並且當膜內ADH添加量上升其對應靈敏度也上升,其應答時間4秒,其靈敏度達830 nA/mM、線性範圍達0.1 mM、偵測極限達3 μM,最後利用此奈米複合材料薄膜進而測量市售台灣啤酒、高梁酒及紅葡萄酒等酒精產品之酒精濃度,證明能有效測量到酒精物質,並且有效排除其餘干擾物。 第二部分中以MWNTs/Nafion奈米複合材料薄膜對於尼古丁(nicotine)進行測量,結果顯示出奈米碳管可催化尼古丁物質之電化學反應,MWNTs/Nafion奈米複合材料修飾電極對於尼古丁的催化表現是由循環伏安法及安培法所證明,奈米碳管在Nafion高分子薄膜之中,依然可以表現出催化尼古丁的特性,選擇出最佳的pH 8.0值及最佳測量電位0.8 V作為測量尼古丁濃度,其奈米複合材料薄膜可以比Nafion薄膜材料提供更低的氧化電位作為偵測尼古丁的氧化反應,並且有抗電極毒化的能力,以最適值所製備出的MWNTs/Nafion奈米複合材料薄膜對於偵測nicotine具有靈敏度達19100 nA/mM,線性範圍達0.4 mM、偵測極限達1.7 μM,干擾物實驗中,證明甲醇不會因奈米碳管而催化,並得知甲醇反應物不會對於測量尼古丁氧化物質時造成干擾,因此提高MWNTs/Nafion薄膜具有做為尼古丁化學感測器的可行性,最後針對所修飾的電極做為測量PALL MALL香菸真實樣品尼古丁值為0.87 mg/pic。zh_TW
dc.description.abstractAn amperometric biosensor for the determination of ethanol has been constructed. It comprises a multiwalled carbon nanotubes (MWNTs) conduit, a Nafion binder, and an alcohol dehydrogenase (ADH) function. The measurement of ethanol is based on the signal produced by β-nicotinamide adenine dinucleotide (NADH), the product of the enzymatic reaction. The MWNTs are cylindrical with an outer diameter in the range 40-60 nm, an inner diameter in the range 2-5 nm, and a length of up to several micrometers. The homogeneity of the resulting nanobiocomposite film was characterized by atomic force microscopy (AFM). The performance of the MWNTs/Nafion/ADH nanobiocomposite modified glassy carbon electrode was examined using cyclic voltammetry and amperometry in presence of NADH and in the presence of ethanol. The electrocatalytic activity of MWNTs towards the oxidation of NADH has allowed an effective low-potential amperometric determination of ethanol. In the case of 6 mgmL-1 ADH, the MWNTs-Nafion-ADH nanobiocomposite film displayed a sensitivity of 830 nAmM-1, a linear range up to 0.1 mM, a detection limit of 3 μM , and a response time of about 4 s. The electrocatalytic activity of MWNTs towards the oxidation of nicotine has allowed an effective low-potential amperometric determination of nicotine. The MWNTs/Nafion nanocomposite film displayed a sensitivity of 19100 nA/mM, a linear range up to 0.4 mM, a detection limit of 1.7 μM. The system was applied to the determination of nicotine in tobacco sample (cigarette) and revealed a nicotine value of about 0.87 mg pic-1 at tobacco sample.en_US
dc.description.tableofcontents目錄 中文摘要.................................................................................................................................................. i 英文摘要 .............................................................................................................................................. iii 目錄......................................................................................................................................................... iv 圖目錄.................................................................................................................................................... vi 表目錄.................................................................................................................................................... xi 第一章 緒論 ......................................................................................................................................... 1 1-1 前言..................................................................................................................................1 1-2 分析物之簡介................................................................................................................ 3 1-2-1 分析物簡介........................................................................................................ 3 1-2-2 分析物偵測之文獻回顧.................................................................................. 5 1-3 生物感測器.................................................................................................................. 11 1-3-1 生物感測器的定義...........................................................................................11 1-3-2 生物感測器基本構造...................................................................................... 12 1-4 電化學分析方法......................................................................................................... 16 1-4-1 循環伏安法....................................................................................................... 16 1-4-2 安培法................................................................................................................ 18 1-5 奈米碳管...................................................................................................................... 20 1-5-1 簡介 .................................................................................................................. 20 1-5-2 奈米碳管的應用.............................................................................................. 22 1-6 全氟磺酸聚合物......................................................................................................... 24 1-7 微觀結構分析............................................................................................................. 27 1-7-1 原子力顯微鏡................................................................................................... 27 第二章 實驗方法與步驟 ............................................................................................................... 29 2-1 實驗藥品........................................................................................................................... 29 2-2 實驗儀器........................................................................................................................... 29 2-3 實驗步驟........................................................................................................................... 30 2-3-1 電極前處理...................................................................................................... 30 2-3-2 MWNTs/Nafion修飾GCE............................................................................ 31 2-3-3 MWNTs/Nafion/ADH修飾GCE................................................................ 32 2-3-4 電化學測量...................................................................................................... 33 第三章 結果與討論....................................................................................................................... 35 3-1 MWNTs/Nafion/ADH薄膜修飾GCE酒精生物感測器....................................... 35 3-1-1 MWNTs/Nafion/ADH薄膜修飾GCE之表面型態分析....................... 35 3-1-2 MWNTs/Nafion薄膜修飾GCE偵測NADH之試驗............................ 41 3-1-3 MWNTs/Nafion/ADH薄膜修飾GCE偵測酒精之試驗....................... 50 3-1-4 MWNTs/Nafion/ADH薄膜修飾GCE偵測真實樣品之試驗 ............................................................................................................................ 61 3-2 MWNTs/Nafion薄膜修飾GCE之尼古丁化學感測器........................................... 66 3-2-1 MWNTs/Nafion薄膜修飾GCE偵測尼古丁之試驗.............................. 66 3-2-2 MWNTs/Nafion薄膜修飾GCE偵測香菸真實樣品之試驗 ............................................................................................................................ 82 第四章 結論與未來展望............................................................................................................... 84 4-1 結論.................................................................................................................................... 84 4-2 未來展望........................................................................................................................... 85 第五章 參考文獻............................................................................................................................ 87zh_TW
dc.language.isoen_USzh_TW
dc.publisher化學工程學系所zh_TW
dc.relation.urihttp://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2806200616071300en_US
dc.subjectcarbon nanotubesen_US
dc.subject奈米碳管zh_TW
dc.subjectbiosensorsen_US
dc.subjectethanolen_US
dc.subjectnicotineen_US
dc.subjectNafionen_US
dc.subject生物感測器zh_TW
dc.subject酒精zh_TW
dc.subject尼古丁zh_TW
dc.subject全氟磺酸聚合物zh_TW
dc.title多層壁奈米碳管/全氟磺酸聚合物複合材料薄膜做為感測器之探討zh_TW
dc.titleSensors Based on Multiwalled Carbon Nanotubes/Nafion Composite Filmen_US
dc.typeThesis and Dissertationzh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeThesis and Dissertation-
item.cerifentitytypePublications-
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.grantfulltextnone-
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