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標題: 由二階段乳化法製備具酸鹼應答之仿生性高分子vesicles及其特性探討
Biomimetic pH-Controllable Polymersomes Prepared by Double Emulsion and their Structural Characterization
作者: 林玥彣
Lin, Yue-Wen
關鍵字: amphiphilic copolymer;雙性高分子;polymersome;polymer vesicle;artificial cell;pH;channel;微脂粒;囊泡;人工細胞;酸鹼應答
出版社: 化學工程學系所
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Graft copolymers comprising poly(acrylic acid) (PAAc) as the backbone and acrylate of 1,2-distearoyl-rac-glycerol (distearin) as the grafts undergo supramolecular assembling by a double emulsion approach into polymeric vesicles (polymersomes). The structure of polymeric aggregates was affected by the degree of substitution (DS, mole percent of the grafted distearin segments) of the graft copolymers. In this study, the graft copolymers with a DS of 10 mol% or above can arrange their structure into vesicle form, but micelles with a DS of 5 mol%. The vesicle sizes can be regulated in the range from 3.3 to 12.7 micrometers in diameter by controlling the volume ratio of THF to chloroform in the oil phase during the polymersome preparation. The size decreased with increasing the content of the water-miscible solvent (THF) in the organic cosolvent system. Due to the stability of the vesicle structure, the giant vesicles containing small vesicles suspended in their interior aqueous compartments that become more resembling the living cells and their intracellular organelles can be prepared by a two-step double emulsion technique. A vesicle structure model has been proposed in this study that the membrane is formed by hydrophobic segregation of polymeric chains into the lipid bilayer and PAAc-enriched regions. With the dissociation degree of the AAc residues being increased by increasing pH from 5.0 to 8.0, the PAAc-enriched regions are transformed into channels and become permeable to hydrophilic solutes. As a result, the channels embedded in vesicle membrane have shown the capability of being switched on and off by pH alternation.
其他識別: U0005-0307200720452600
Appears in Collections:化學工程學系所

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