Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3605
標題: 以多層壁奈米碳管做為感測器材料之探討
Sensing materials based on multiwalled carbon nanotubes
作者: 邱乾城
Chiu, Chian-Cheng
關鍵字: Biosensor;安培法;Amperometry;Multiwalled carbon nanotubes;Tyrosinase;Nafion;多層壁奈米碳管;酪胺酸酶全氟磺酸聚合物
出版社: 化學工程學系所
引用: [1] 劉東山,黃政賢,環境工程學,曉園出版社有限公司,臺北,1990。 [2] 陳靜生,水環境化學,曉園出版社有限公司,臺北,1992。 [3] 盧昭彰,環境化學概論,曉園出版社有限公司,臺北,1995。 [4] R. Gatti, M.G. Gioia, A.M. Di Pietra, V. Cavrini, Anal. Chim. Acta, 447, 89, 2001. [5] M. Castillo, R. Domingues, M.F. Alpendurada, D. Barceló, Anal. Chim. Acta, 353, 133, 1997. [6] C.D. Chriswell, R.C. Chang, J.S. Frltz, Anal. Chem., 47, 1325, 1975. [7] M. Naczk, F. Shahidi, J. Chromatogr. A, 1054, 95, 2004. [8] L.C. Jr Clark, C. Lyons, Ann. N.Y. Acad. Sci., 102, 29, 1962. [9] S. Iijima, Nature, 354, 56, 1991. [10] M.C. Bank, R.R. Moore, T.J. Davies, R.G. Compton, Chem. Commu., 1805, 2004. [11] R.R. Moore, C.E. Banks, R.G. Compton, Anal. Chem.,76, 2677, 2004. [12] J. Wang, M. Musameh, A. Merkoci, Y. Lin, Electrochem. Commu., 4, 743, 2002. [13] S.G. Wang, Q. Zhang, R. Wang, S.F. Yoon, Biochem. Biophys. Res. Commun., 311, 572, 2003. [14] Y.C. Tsai, S.C. Li, J.M. Chen, Langmuir, 21, 3653, 2005. [15] X. Yu, D. Chattopadhyay, I. Galeska, F. Papadimitrakopoulos, J.F. Rusling, Electrochem. Commun., 5, 408, 2003. [16] K. Yamamoto, G.Y. Shi, T.S. Zhou, F. Xu, J.M. Xu, T. Kato, J.Y. Jin, L.T. Jin, Analyst, 128, 249, 2003. [17] H.W. Liaw, J.M. Chen, Y.C. Tsai, J. Nanosci. Nanotechnol., 6, 2396, 2006. [18] M. Musameh, J. Wang, A. Merkoci, Y. Lin, Electrochem. Commun., 4, 743, 2002. [19] J. Zhu, M. Yudasaka, M. Zhang, S. Iijima, J. Phys. Chem. B, 108, 11317. [20] 物質安全資料表http://www.iosh.gov.tw/data/f11/msdsc.htm [21] Wikipedia 維基中文免費百科全書http://www.babylon.com/dictionary/55862/Wikipedia [22] B.R. Eggins, Chemical Sensors and Biosensors, Wiley, New York, 2002. [23] J. Shah, E. Wilkins, Electroanalysis, 15, 157, 2002. [24] D. L. Polla, L. F. Francis, MRS Bulletin, 59, 1996. [25] M. Okuyama, IEEE International Symposium on Micromechatronics and Human Science, 29, 1998. [26] E. Bakker, P. Bühlmann, E. Pretsch, Electroanalysis, 11, 915, 1999. [27] Biochemistry Basic: http://juang.bst.ntu.edu.tw/BCbasics/Enzyme12.htm [28] R. A. Messing, Academic Press, New York, p1, 1975. [29] F.T. Richard, S.S. Jerome, Handbook of Chemical and Biological Sensors, p5. [30] J.M.S. Cabral, J.F. Kennedy, R. F. Taylor(New York; Dekker), 73,1991. [31] J.M.S. Cabral, J.F. Kennedy, R. F. Taylor(New York; Dekker), 73,1991. [32] J.C. Vidal, E. Garcia, J.R. Castillo, Sensors and Actuators B, 57,219,1999. [33] U. Mirtha, W. Jess, Anal. Chem., 58,2979,1986. [34] L. Doretti, D. Ferrara, P. Gattolin, S. Lora, Talanta, 44, 859, 1997. [35] G.T. Constantinos, B.F. Ageliki, N.T. Pantelis, Electro. Commu., 7, 781, 2005. [36] D. Lucio, F. Daniela, G. Paola, L. Silvano, Talanta, 44, 859, 1997. [37] J. Parellada, A. Narvaez, Biosens. Bioelectron., 12, 267, 1997. [38] J. Li, L.S. Chia, N.K. Goh, S.N. Tan, J. Electroanal. Chem., 460, 234, 1999. [39] 呂淑佩,聚苯胺複合式酵素碳粉電極在生化分析上的應用,國立東華大學化學研究所,2002。 [40] A.J. Bard, I.R. Faulkner, Electrochemical Methods: Fundaments and Applications, Wily, New York, 2000. [41] D.R. Crow, Principle and Applications of Electrochemistry,高立,1998。 [42] F.M. Veronese, C. Mammucari, F. Schiavon, O. Schiavon, S. Lora, F. Secundo, A. Chilin, A. Guiotto, Il Farmaco, 56, 541, 2001. [43] 胡啟章,電化學原理與方法,五南圖書,2002。 [44] Rice University: Rick Smalley’s Group Home Page-Image Gallery. http://smalley.rice.edu/index.cfm [45] C.N.R. Rao, B.C. Satishkumar, A. Govindaraj, M. Nath, Chemphyschem, 2, 78, 2001. [46] D.S. Bethune, C.H. Kiang, M.S. Devries, G. Gorman, R. Savoy, J. Vazquez, Nature, 363, 605, 1993. [47] C.N.R. Rao, B.C. Satishkumar, A. Govindaraj, M. Nash, Chem. Phys. Chem., 2, 78, 2001. [48] N. Hamada, S. Sawada, A. Oshiyama, Phys. Rev. Lett., 68,1579,1992. [49] R.Saito, M. Fujita, G. Dresselhaus, M. S. Dresslhaus, Appl. Rev. Lett., 60,2204,1992. [50] J.W.G. Wildoer, L.C. Venema, A.G. Rinzler, R.E. Smalley, C. Dekker, Nature, 391, 59, 1998. [51] J. E. Fischer, Acc. Chem. Res., 35,1079,2002. [52] M. Terrones, W. K. Hsu, H.W. Kroto, D. R. M. Walton, Topics in Current Chemistry, 1991,1,1998. [53] J. Hone, M. Whitney, C. Piskoti, A. Zettl, Phys. Rev. B, 59,2514,1999. [54] M. A. Osman, D. Srivastava, Nanotechnolory, 12, 21 (2001). [55] M. R. Falvo, G. J. Clary, R. M. II Taylor, V. Chi, F. P. Brooks, S. Washburn, R. Superfine, Nature, 277, 1971, 1997. [56] M.M.J. Treacy, T.W. Ebbesen, J.M. Gibson, Nature, 381, 678, 1996. [57] E.W. Wong, P.E. Sheehan, C.M. Lieber, Science, 277, 1971, 1997. [58] M.R. Pederson, J.Q. Broughton, Physical Review Letters, 69, 2689, 1992. [59] A. C. Dillon, K. M. Jones, T. A. Bekkedahl, C. H. Kiang, D. S. Bethune, M. J. Heben, Nature, 386, 377, 1997. [60] G. T. Wu, C. S. Wang, X. B. Zhang, H. S. Yang, Z. F. Qi, W. Z. Li, J. Power Sources, 75, 175, 1998. [61] F. Beguin, V. A. Nalimova, D. E. Sklovsky, G. N. Bondarenko, H. Alvergnat-Gaucher, S. Bonnamy, Synthetic Metals, 88, 89, 1997 . [62] C.C. Pang, Min.H. Chen, T.Y. Lin, T.C. Chou, Sensor and Actuators B, 73, 221, 2001. [63] P. C. Pandey, S. Upadhyay, Ida Tiwari, V. S. Tripathi, Anal. Biochem., 288, 39, 2001. [64] X. Yu, D. Chattopadhyay, I. Galeska, F. Papadimitrakopoulos, J.F. Rusling, Electrochem. Commun., 5, 408, 2003. [65] M. Umana, J. Waller, Anal. Chem., 58, 2979, 1986. [66] M.C. Shin, H.S. Kim, Biosens. Bioelectron., 11, 171, 1996. [67] K. Yamamoto, G.Y. Shi, T.S. Zhou, F. Xu, J.M. Xu, T. Kato, J.Y. Jin, L.T. Jin, Analyst, 128, 249, 2003. [68] X. Yu, G. A. Sotzing, F. Papadimitrakopoulos, J. F. Rusling, Anal. Chem., 75, 4565, 2003. [69] K. Wu, J. Fei, S. Hu, Anal. Biochem., 318, 100, 2003. [70] T. N. Rao, I. Yagi, T. Miwa, D.A. Tryk, A. Fujishima, Anal. Chem., 71, 2506, 1999. [71] K.P. Troyer, R.M. Wightman, Anal. Chem., 74, 5370, 2002. [72] J. Wang, M. Li, Z. Shi, N. Li, Z. Gu, Microchem. J., 73, 325, 2002. [73] T. Nakaminami, S. Kuwabata, H. Yoneyama, Anal. Chem., 71, 1928, 1999. [74] J. Wang, M.P. Chatrathi, B. Tian, R. Polsky, Anal.Chem., 72, 2514, 2000. [75] A. Hirsch, Angew. Chem. Int. Ed., 41, 1853, 2002. [76] J. Liu, A.G. Rinzler, H. Dai, J.H. Hafner, R.K. Bardley, P.J. Boul, A. Lu, T. Iverson, K. Shelimov, C.B. Huffman, F.R. Macias, Y.S. Shon, T.R. Lee, D.T. Colbert, R.E. Smalley, Science, 280, 1253, 1998. [77] A. Kuznetsova, I. Popova, J.T. Yates, M.J. Bronikowski, C.B. Huffman, J. Liu, R.E. Smally, H.H. Hwu, J.G. Chen, J. Am. Chem. Soc., 123, 10699, 2001. [78] A. Kuznetsova, D.B. Mawhinney, V. Naumenko, J.T. Yates, J. Liu, R.E. Smalley, Chem. Phys. Lett., 321, 292, 2000. [79] E.T. Mickelson, I.W. Chiang, J.L. Zimmerman, P.J. Boul, J. Lozano, J. Liu, R.E. Smally, R.H. Hauge, J.L. Margrave, J. Phys. Chem. B, 103, 4318, 1999. [80] P.J. Boul, J. Liu, E.T. Mickelson, C.B. Huffman, L.M. Ericson, I.W. Chiang, K.A. Smith, D.T. Colbert, R.H. Hauge, J.L. Margrave, R.E. Smally, Chem. Phys. Lett., 310, 367, 1999. [81] M.J. O’Connell, S.M. Bachilo, C.B. Huffman, V.C. Moore, M.S. Strano, E.H. Haroz, K.L. Rialon, P.J. Boul, W.H. Noon, C. Kittrell, J. Ma, R.H. Hauge, R.B. Weisman, R.E. Smalley, Science, 297, 593, 2002. [82] M.J. O’Connell, P. Boul, L.M. Ericson, C. Huffman, Y. Wang, E. Haroz, C. Kuper, J. Tour, K.D. Ausman, R.E. Smalley, Chem. Phys. Lett., 342, 265, 2001. [83] J.E. Riggs, Z. Guo, D.L. Carroll, Y.P. Sun, J. Am. Chem. Soc., 122, 5879, 2000. [84] A. Star, J.F. Stoddart, D. Steuerman, M. Diehl, A. Boukai, E.W. Wong, X. Yang, S.W. Chung, H. Choi, J.R. Heath, Angew. Chem. Int. Ed., 40, 1721, 2001. [85] A. Star, D.W. Steuerman, J.R. Heath, J.F. Stoddart, Angew. Chem. Int. Ed., 41, 2508, 2002. [86] J. Wang, M. Musameh, Y. Lin, J. Am.Chem. Soc., 125, 2408, 2003. [87] DuPont: http://www.dupont.com.tw [88] B. Hoyer, T.M. Florence, G.E. Batley, Anal. Chem., 59, 1608, 1987. [89] J. Wang, Analytical Electrochemistry, Second Edition, Wiley-VCH, New York, 2000. [90] D.J. Harrison, R.F.B. Turner, H.P. Baltes, Anal. Chem., 60, 2002, 1988. [91] J.M. Zen M.L. Lee, Anal. Chem., 65, 3238, 1993. [92] Z. Chen, Z. Pourabedi, D.B. Hibbert, Electroanalysis, 11, 964, 1999. [93] B. Hoyer, N. Jensen, L.P. Busch, Electroanalysis, 13,843, 2000. [94] K. Crowley, J. Cassidy, Electroanalysis, 14, 1077, 2001. [95] G. Kefala, A. Econmou, A. Voulgaropoulos, Analyst, 129, 1082, 2004. [96] V. Tohver, J. E. Smay, A. Braem, P. V. Braun, J. A. Lewis, Proc. Natl. Acad. Sci. U. S. A., 98, 8950, 2001. [97] V. Tohver, A. Chan, O. Sakurada, J. A. Lewis, Langmuir, 17, 8414, 2001. [98] Z. Liu, B. Liu, J. Kong, J. Deng, Anal. Chem., 72, 4707, 2000. [99] G. Binning, C.F. Quate, Ch. Gerber, Phys. Rev. Lett., 56, 930, 1986. [100] 洪郁婷,氮化物的奈米結構,國立臺灣大學化學工程學系碩士論文,2001. [101] Y.C. Tsai, J.M. Chen, F. Marken, Microchim. Acta, 150, 269, 2005. [102] A. Naidja, C. Liu, P. M. Huang, J. Colloid Interface Sci., 251, 46, 2002. [103] B.R. Azamian, J.J. Davis, K.S. Coleman, C.B. Bagshaw, M.L.H. Green, J. Am. Chem. Soc., 124, 12664, 2002. [104] Q. Zhao, Z. Gan, Q. Zhuang, Electroanalysis, 14, 1609, 2002. [105] S. Zhang, H. Zhao, R. John, Electroanalysis, 17, 239, 2005. [106] N. Li, M.H. Xue, H. Yao, J.J. Zhu, Anal. Bioanal. Chem., 383, 1127, 2005. [107] N. Peña, A.J. Reviejo, J.M. Pingarrón, Talanta, 55, 179, 2001. [108] K.S. Booksh, B.R. Kowalski, Anal. Chem., 782A, 66, 1994. [109] A.J. Cunningham, Introduction to Bioanalytical Sensors, Wiley, New York, 1998. [110] Y.C. Tsai, S.C. Li, J.M. Chen, Langmuir, 21, 3653, 2005. [111] S.S. Razola, B.L. Ruiz, N.M. Diez, H.B. Jr Mark, J.-M. Kauffmann, Biosens. Bioelectron., 17, 921 2002. [112] M. Vreeke, R. Maidan, A. Heller, Anal. Chem., 64, 3084, 1992. [113] M. Elmgren, S.-E. Lindquist, M. Sharp, J. Electroanal. Chem., 362, 227, 1993. [114] P. Önnerfjord, J. Emnéus, G. Marko-Varga, L. Gorton, Biosens. Bioelectron., 10, 607, 1995. [115] F. Liu, A.J. Reviejo, J.M. Pingarrón, J. Wang, Talanta, 41, 455, 1994. [116] B. Serra, S. Jimenez, M.L. Mena, A.J. Reviejo, J.M. Pingarrón, Biosens. Bioelectron., 17, 217, 2002.
摘要: 
本實驗成功研究製備出以多層壁奈米碳管-全氟磺酸聚合物-酪胺酸酶(MWNTs-Nafion-Tyrosinase)生物奈米複合材料薄膜修飾玻璃碳電極(glassy carbon electrode, GCE),證明具有偵測酚化合物(phenolic compounds: phenol, o-cresol, p-cresol, m-cresol, catechol, dopamine and ephinephrine)的能力。其中MWNTs具有優勢的電化學催化活性特性並且成為奈米導線複合材料,Nafion高分子薄膜則為電化學改良聚合黏結劑,而Tyrosinase則為一生物性觸媒,能以低活化能促進酚化合物轉化為鄰苯二酚 (catechol),而鄰苯二酚再經由Tyrosinase催化產生o-quinones,最後o-quinones由電化學還原反應得到電流訊號。利用原子力顯微鏡(atomic force microscope, AFM)來確認所製備出來的生物奈米複合材料薄膜為均勻的,並觀察由電弧放電法合成的MWNTs為筆直的柱狀結構,且每根MWNTs 都均勻的分布在Nafion薄膜中成為奈米導線,提供了相當良好的電傳導性。將MWNTs-Nafion-Tyrosinase生物奈米複合材料和Nafion-Tyrosinas生物複合材料分別修飾於GCE上,利用電化學法中的循環伏安法(cyclic voltammetry)和安培法(amperometry)偵測酚(phenol)做比較,可以發現有MWNTs修飾的電極具有較大的電流信號及效能,在酚生物感測器中,選擇-10 mV為最適操作電壓,添加2 mg/mL Tyrosinase 為偵測之最佳條件,其應答時間8秒、線性範圍可達19 μM、靈敏度303 μA mM-1和偵測極限為0.13 μM。MWNTs-Nafion-Tyrosinase生物奈米複合材料修飾於GCE,應用於偵測酚化合物上也有顯著的效果。

Novel amperometric biosensors for the determination of phenolic compounds (phenol, o-cresol, p-cresol, m-cresol, catechol, dopamine, and ephinephrine) have been constructed. It comprises a multiwalled carbon nanotube (MWNTs) conduit, a Nafion backbone, and a tyrosinase function. The modification of glassy carbon electrode (GCE) is made by a simple solution-evaporation method. The resulting multiwalled carbon nanotube-Nafion-tyrosinase (MWNTs-Nafion-Tyr) nanobiocomposite film modified electrode is inexpensive, reliable, and easy to use. The homogeneity of the MWNTs-Nafion-Tyr nanobiocomposite films was characterized by atomic force microscopy (AFM). The measurement of phenolic compounds is based on the signal produced by the electrochemical reduction of o-quinones, the product of the enzymatic reaction. Optimization studies were performed in stirred solution and the magnitude of response currents were found to be dependent on applied potential (-10 mV) and enzyme loading (2 mg/mL Tyrosinase). The optimized biosensor for phenol determination displayed a sensitivity of 310 μA mM-1, a linear range up to 19 μM, a detection limit of 0.13 μM, and a response time of less than 8 s.
URI: http://hdl.handle.net/11455/3605
其他識別: U0005-0407200717292200
Appears in Collections:化學工程學系所

Show full item record
 

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.