傳染性華氏囊病毒的VP2蛋白 N端及C端序列對於T=1 次病毒顆粒的組裝與純化及免疫原性之影響

何靜宜; Ho, Jin-Yi

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Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/36128

DC Field	Value	Language
dc.contributor	王國祥	zh_TW
dc.contributor	李龍湖	zh_TW
dc.contributor	劉秉慧	zh_TW
dc.contributor.advisor	王敏盈	zh_TW
dc.contributor.author	何靜宜	zh_TW
dc.contributor.author	Ho, Jin-Yi	en_US
dc.contributor.other	中興大學	zh_TW
dc.date	2007	zh_TW
dc.date.accessioned	2014-06-06T07:53:55Z	-
dc.date.available	2014-06-06T07:53:55Z	-
dc.identifier	U0005-2708200617432600	zh_TW
dc.identifier.citation	林育江。2003。傳染性華氏囊病毒結構蛋白VP2之C端區域對形成次病毒顆粒及免疫力之影響。碩士論文。中興大學生物科技所。台中。楊道翔。2005。利用昆蟲幼蟲生產傳染性華氏囊炎病毒結構蛋白VP2之次病毒顆粒及其應用。碩士論文。中興大學生物科技所。台中。 Azad, A. A., S. A. Barrett, and K. J. Fahey. 1985. The characterization and molecular cloning of the double-stranded RNA genome of an Australian strain of infectious bursal disease virus. Virology 143:35-44. Azad, A. A., M. N. Jagadish, M. A. Brown, and P. J. Hudson. 1987. Deletion mapping and expression in Escherichia coli of the large genomic segment of a birnavirus. Virology 161:145-52. Baumert, T. F., S. Ito, D. T. Wong, and T. J. Liang. 1998. Hepatitis C virus structural proteins assemble into viruslike particles in insect cells. J. Virol. 72:3827-36. Becht, H., H. M	zh_TW
dc.identifier.uri	http://hdl.handle.net/11455/36128	-
dc.description.abstract	VP2蛋白是傳染性華氏囊病病毒的主要結構蛋白之ㄧ，亦是誘發宿主產生中和性抗體的免疫原。昆蟲細胞表現系統VP2蛋白(452H)會自我組裝形成T=1的次病毒顆粒。目前次病毒顆粒的結構已獲得2.6	zh_TW
dc.description.abstract	VP2 is the structure proteins of IBDV capsid and the primary host-protective immunogen of IBDV. When expressed in insect cell, VP2-452H spontaneously forms a T=1 subviral particle (SVP). Although the structure of SVP has been determined at 2.6	en_US
dc.description.tableofcontents	圖次………………………………………………………………………4 表次…………………………………………………………………… 7 中文摘要…………………………………………………………………9 英文摘要...............................................10 第壹章、前言………………………………………………………….12 1. 家禽傳染性華氏囊病病毒之背景介紹…………………………12 2. 家禽傳染性華氏囊病病毒基因體組成及蛋白產物……………13 3. 病毒顆粒立體結構之研究………………………………………16 4. 以次病毒顆粒當作疫苗或載體…………………………………21 5. 研究動機及目的…………………………………………………23 第貳章、材料與方…………………………………………………….25 1. 細胞與病毒………………………………………………………25 2. 抗體的製備………………………………………………………28 3. 重組病毒之構築及製備…………………………………………28 4. 重組蛋白之表現…………………………………………………32 5. 重組蛋白之分析與鑑定…………………………………………33 6. 重組蛋白之純化…………………………………………………35 7. 穿透式電子顯微鏡觀察…………………………………………36 8. 雞隻之免疫試驗…………………………………………………37 9. 免疫試驗之分析............... ..............38 10. 以次病毒顆粒抑制IBDV感染DF-1細胞…………………………40 第	zh_TW
dc.language.iso	en_US	zh_TW
dc.publisher	生物科技學研究所	zh_TW
dc.relation.uri	http://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-2708200617432600	en_US
dc.subject	IBDV	en_US
dc.subject	傳染性華氏囊病毒	zh_TW
dc.subject	virus-like particle	en_US
dc.subject	truncation	en_US
dc.subject	IMAC	en_US
dc.subject	似病毒顆粒	zh_TW
dc.subject	截切	zh_TW
dc.subject	固定化金屬層析	zh_TW
dc.title	傳染性華氏囊病毒的VP2蛋白 N端及C端序列對於T=1 次病毒顆粒的組裝與純化及免疫原性之影響	zh_TW
dc.title	Effect of terminal sequences of capsid protein VP2 of infectious bursal disease virus on the formation, purification and immunogenicity of T=1 subviral particles	en_US
dc.type	Thesis and Dissertation	zh_TW
item.openairetype	Thesis and Dissertation	-
item.openairecristype	http://purl.org/coar/resource_type/c_18cf	-
item.languageiso639-1	en_US	-
item.grantfulltext	none	-
item.fulltext	no fulltext	-
item.cerifentitytype	Publications	-
Appears in Collections:	生物科技學研究所

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