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標題: 應用液態培養Ganoderma lucidum生產Polysaccharide-油脂及界面活性劑添加效應之探討
Production of Polysaccharide by Ganoderma lucidum in Submerged-Study on fatty acids and surfactants addition effects.
作者: 洪嘉男
Hung, Chia-Nan
關鍵字: Ganoderma lucidum;靈芝;polysaccharide;submerged culture;lipids;surfactants;多醣體;液態醱酵;油脂;界面活性劑
出版社: 化學工程學系所
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靈芝(Ganoderma lucidum)是中藥上常用的一種藥用真菌,根據文獻指出,其所生產之胞外多醣體具有抗腫瘤之療效。本研究利用界面劑和油脂的添加進行靈芝菌絲體之深層培養,進而提升菌體乾重及多醣體之產量。
研究發現,界面劑Span 80的添加對於菌體乾重和多醣體的產量皆具提升效果,添加2%Span80之菌體乾重為9.41 g/L,為未添加的1.72倍,同時多醣體的產量由341.99 mg/L 提升至 536.92 mg/L;在油脂的添加方面,橄欖油的添加有助於菌體乾重的提升,而玉米油的添加對於多醣體的產量是有提升的效果為未添加的2.1倍。於第0天添加2%玉米油、1%橄欖油及起始pH值6下,以轉速100 rpm的條件進行培養,其結果發現,菌體乾重及多醣體之產量都有提升的效果,多醣體的產量約為未添加時的4.74倍,而菌體乾重約為未添加時的3倍;另一方面,於第12天提昇轉速至200 rpm,其多醣體產量於第13天可達1810 mg/L。


Ganoderma lucidum is one of the most famous traditional Chinese medicinal herbs. It was reported that polysaccharide isolated from fruiting body and the mycelia culture of G. lucidum has immunomodulating and anti-tumor activity. The object of this study was to enhance mycelia and polysaccharide production in submerged culture by addition of surfactants or oils.
In supplementation of surfactant experiments, Span 80 have positive effect on dry cell concentration and polysaccharide production. The dry cell concentration was 9.41g/L by addition of 2% Span 80, 1.72 times to that of control. Meanwhile, the polysaccharide production was enhanced from 341.99mg/L to 536.92mg/L. In experiments of oils supplementation, addition of olive oil was favor to mycelia growth. In contrast, supplementation of the corn oil increased the polysaccharide production (2.1 times compared to control). Based on the above results, a fermentation was carried out by adding 2% corn oil and 1% olive oil, and the initial pH and rotation speed was 6 and 100 rpm, respectively. The results showed that the mycelia as well as polysaccharide production was dramatically improved (3.0 and 4.74 times compared to control respectively). On the other hand, to enhance polysaccharide production, experiments were conducted by adiusting the rotation rates to alter the share force excenting on the strain. When the rotation rate was changed from 100 to 200 rpm at 12 days' cultivation, the results showed that polysaccharide production increased to 1810 mg/L at day 13, a 5.3 times increase as compared to that of control.
其他識別: U0005-0607200717381700
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