Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/36140
標題: 文心蘭中B功能性MADS Box基因之選殖與特性分析
Molecular Cloning and Characterization of B function MADS Box Genes from Orchid (Oncidium Gower Ramsey)
作者: 高乃萱
Kao, Nai-Hsuan
關鍵字: MADS box;文心蘭;B-function genes;花器;唇辦
出版社: 生物科技學研究所
引用: 吳家偉 (2005) 文心蘭中B和E功能性之MADS box開花基因之選殖與特性分析。 國立中興大學生物科技學研究所碩士論文。 徐杏芬 (2003) 文心蘭花朵發育相關之MADS box基因之選殖及功能分析。 國立中興大學生物科技學研究所博士論文。 謝文蘋 (2006) 百合MADS Box基因之功能性分析及其相互作用以調控花器形成機制之探討。 國立中興大學生物科技學研究所碩士論文。 Asada, Y., Kasai, N., AdachiI, Y., Kanno, A., Ito, N., Yun, P.-Y., and Masuda, K. (2006). A vegetative line of asparagus (Asparagus officinalis) with a homeotic change in flower development is correlated with a functional deficiency in class-B MADS-box genes. The Journal of Horticultural Science and Biotechnology 81, 874-882. Adam, H., Jouannic, S., Morcillo, F., Richaud, F., Duval, Y., and Tregear, J.W. (2006). MADS box genes in oil palm (Elaeis guineensis): patterns in the evolution of the SQUAMOSA, DEFICIENS, GLOBOSA, AGAMOUS, and SEPALLATA subfamilies. Journal of Molecular Evolution 62, 15-31. Ambrose, B.A., Lerner, D.R., Ciceri, P., Padilla, C.M., Yanofsky, M.F., and Schmidt, R.J. (2000). Molecular and genetic analyses of the silky1 gene reveal conservation in floral organ specification between eudicots and monocots. Molecular Cell 5, 569-579. Berbel, A., Navarro, C., Ferrandiz, C., Canas, L.A., Beltran, J.P., and Madueno, F. (2005). Functional conservation of PISTILLATA activity in a pea homolog lacking the PI motif. Plant physiology 139, 174-185. Bowman, J.L., Smyth, D.R., and Meyerowitz, E.M. (1989). Genes directing flower development in Arabidopsis. The Plant Cell 1, 37-52. Coen, E.S., and Meyerowitz, E.M. (1991). The war of the whorls: genetic interactions controlling flower development. Nature 353, 31-37. Ditta, G., Pinyopich, A., Robles, P., Pelaz, S., and Yanofsky, M.F. (2004). The SEP4 gene of Arabidopsis thaliana functions in floral organ and meristem identity. Curr Biol 14, 1935-1940. Favaro, R., Pinyopich, A., Battaglia, R., Kooiker, M., Borghi, L., Ditta, G., Yanofsky, M.F., Kater, M.M., and Colombo, L. (2003). MADS-box protein complexes control carpel and ovule development in Arabidopsis. The Plant cell 15, 2603-2611. Gietz, D., St Jean, A., Woods, R.A., and Schiestl, R.H. (1992). Improved method for high efficiency transformation of intact yeast cells. Nucleic acids research 20, 1425. Höfgen, R., and Willmitzer, L. (1988). Storage of competent cells for Agrobacterium transformation. Nucleic acids research 16, 9877. Honma, T., and Goto, K. (2001). Complexes of MADS-box proteins are sufficient to convert leaves into floral organs. Nature 409, 525-529. Hsu, H.F., and Yang, C.H. (2002). An orchid (Oncidium Gower Ramsey) AP3-like MADS gene regulates floral formation and initiation. Plant & Cell physiology 43, 1198-1209. Jack, T., Brockman, L.L., and Meyerowitz, E.M. (1992). The homeotic gene APETALA3 of Arabidopsis thaliana encodes a MADS box and is expressed in petals and stamens. Cell 68, 683-697. Kanno, A., Park, J.H., Ochiai, T., and Kameya, T. (2004). Folral organ identity genes involved in petal development in asparagus. Flower. Newsl. 38, 10-18. Kanno, A., Nakada, M., Akita, Y., and Hirai, M. (2007). Class B gene expression and the modified ABC model in nongrass monocots. The scientific world journal 7, 268-279. Kanno, A., Saeki, H., Kameya, T., Saedler, H., and Theissen, G. (2003). Heterotopic expression of class B floral homeotic genes supports a modified ABC model for tulip (Tulipa gesneriana). Plant molecular biology 52, 831-841. Kramer, E.M., Dorit, R.L., and Irish, V.F. (1998). Molecular evolution of genes controlling petal and stamen development: duplication and divergence within the APETALA3 and PISTILLATA MADS-box gene lineages. Genetics 149, 765-783. Kramer, E.M., Su, H.J., Wu, C.C., and Hu, J.M. (2006). A simplified explanation for the frameshift mutation that created a novel C-terminal motif in the APETALA3 gene lineage. BMC evolutionary biology 6, 30. Lamb, R.S., and Irish, V.F. (2003). Functional divergence within the APETALA3/PISTILLATA floral homeotic gene lineages. Proceedings of the national academy of sciences of the United States of America 100, 6558-6563. McGonigle, B., Bouhidel, K., and Irish, V.F. (1996). Nuclear localization of the Arabidopsis APETALA3 and PISTILLATA homeotic gene products depends on their simultaneous expression. Genes & development 10, 1812-1821. Moon, Y.H., Jung, J.Y., Kang, H.G., and An, G. (1999). Identification of a rice APETALA3 homologue by yeast two-hybrid screening. Plant molecular biology 40, 167-177. Mukherjee, S., Bal, S., and Saha, P. (2001). Protein interaction maps using yeast two-hybrid assay. Current science 81, 458-464. Nakada, M., Komatsu, M., Ochiai, T., Ohtsu, K., Nakazono, M., Nishizawa, N.K., Ko Nittad, R.N., Kameyaa, T., and Kannoa, A. (2006). Isolation of MaDEF from Muscari armeniacum and analysis of its expression using laser microdissection. Plant Science 170, 143-150 Nakamura, T., Fukuda, T., Nakano, M., Hasebe, M., Kameya, T., and Kanno, A. (2005). The modified ABC model explains the development of the petaloid perianth of Agapanthus praecox ssp. orientalis (Agapanthaceae) flowers. Plant molecular biology 58, 435-445. Norman, C., Runswick, M., Pollock, R., and Treisman, R. (1988). Isolation and properties of cDNA clones encoding SRF, a transcription factor that binds to the c-fos serum response element. Cell 55, 989-1003. Passmore, S., Maine, G.T., Elble, R., Christ, C., and Tye, B.K. (1988). Saccharomyces cerevisiae protein involved in plasmid maintenance is necessary for mating of MAT alpha cells. Journal of molecular biology 204, 593-606. Pelaz, S., Ditta, G.S., Baumann, E., Wisman, E., and Yanofsky, M.F. (2000). B and C floral organ identity functions require SEPALLATA MADS-box genes. Nature 405, 200-203. Pelaz, S., Gustafson-Brown, C., Kohalmi, S.E., Crosby, W.L., and Yanofsky, M.F. (2001). APETALA1 and SEPALLATA3 interact to promote flower development. Plant J 26, 385-394. Pinyopich, A., Ditta, G.S., Savidge, B., Liljegren, S.J., Baumann, E., Wisman, E., and Yanofsky, M.F. (2003). Assessing the redundancy of MADS-box genes during carpel and ovule development. Nature 424, 85-88. Pnueli, L., Abu-Abeid, M., Zamir, D., Nacken, W., Schwarz-Sommer, Z., and Lifschitz, E. (1991). The MADS box gene family in tomato: temporal expression during floral development, conserved secondary structures and homology with homeotic genes from Antirrhinum and Arabidopsis. Plant J 1, 255-266. Purugganan, M.D. (1997). The MADS-box floral homeotic gene lineages predate the origin of seed plants: phylogenetic and molecular clock estimates. Journal of molecular evolution 45, 392-396. Riechmann, J.L., Krizek, B.A., and Meyerowitz, E.M. (1996a). Dimerization specificity of Arabidopsis MADS domain homeotic proteins APETALA1, APETALA3, PISTILLATA, and AGAMOUS. Proceedings of the National Academy of Sciences of the United States of America 93, 4793-4798. Riechmann, J.L., Wang, M., and Meyerowitz, E.M. (1996b). DNA-binding properties of Arabidopsis MADS domain homeotic proteins APETALA1, APETALA3, PISTILLATA and AGAMOUS. Nucleic acids research 24, 3134-3141. Schwarz-Sommer, Z., Huijser, P., Nacken, W., Saedler, H., and Sommer, H. (1990). Genetic Control of Flower Development by Homeotic Genes in Antirrhinum majus. Science 250, 931-936. Sommer, H., Beltran, J.P., Huijser, P., Pape, H., Lonnig, W.E., Saedler, H., and Schwarz-Sommer, Z. (1990). Deficiens, a homeotic gene involved in the control of flower morphogenesis in Antirrhinum majus: the protein shows homology to transcription factors. The EMBO journal 9, 605-613. Theissen, G. (2001). Development of floral organ identity: stories from the MADS house. Current opinion in plant biology 4, 75-85. Theissen, G., and Saedler, H. (1999). The golden decade of molecular floral development (1990-1999): A cheerful obituary. Dev Genet 25, 181-193. Theissen, G., and Saedler, H. (2001). Plant biology: Floral quartets. Nature 409, 469-471. Theissen, G., Kim, J.T., and Saedler, H. (1996). Classification and phylogeny of the MADS-box multigene family suggest defined roles of MADS-box gene subfamilies in the morphological evolution of eukaryotes. Journal of molecular evolution 43, 484-516. Theissen, G., Becker, A., Di Rosa, A., Kanno, A., Kim, J.T., Munster, T., Winter, K.U., and Saedler, H. (2000). A short history of MADS-box genes in plants. Plant molecular biology 42, 115-149. Tsai, W.C., Kuoh, C.S., Chuang, M.H., Chen, W.H., and Chen, H.H. (2004). Four DEF-like MADS box genes displayed distinct floral morphogenetic roles in Phalaenopsis orchid. Plant & cell physiology 45, 831-844. Tsai, W.C., Lee, P.F., Chen, H.I., Hsiao, Y.Y., Wei, W.J., Pan, Z.J., Chuang, M.H., Kuoh, C.S., Chen, W.H., and Chen, H.H. (2005). PeMADS6, a GLOBOSA/PISTILLATA-like gene in Phalaenopsis equestris involved in petaloid formation, and correlated with flower longevity and ovary development. Plant & cell physiology 46, 1125-1139. Tzeng, T.Y., and Yang, C.H. (2001). A MADS box gene from lily (Lilium Longiflorum) is sufficient to generate dominant negative mutation by interacting with PISTILLATA (PI) in Arabidopsis thaliana. Plant & cell physiology 42, 1156-1168. Tzeng, T.Y., Liu, H.C., and Yang, C.H. (2004). The C-terminal sequence of LMADS1 is essential for the formation of homodimers for B function proteins. The Journal of biological chemistry 279, 10747-10755. Whipple, C.J., Ciceri, P., Padilla, C.M., Ambrose, B.A., Bandong, S.L., and Schmidt, R.J. (2004). Conservation of B-class floral homeotic gene function between maize and Arabidopsis. Development (Cambridge, England) 131, 6083-6091. Winter, K.U., Weiser, C., Kaufmann, K., Bohne, A., Kirchner, C., Kanno, A., Saedler, H., and Theissen, G. (2002). Evolution of class B floral homeotic proteins: obligate heterodimerization originated from homodimerization. Molecular biology and evolution 19, 587-596. Xu, Y., Teo, L.L., Zhou, J., Kumar, P.P., and Yu, H. (2006). Floral organ identity genes in the orchid Dendrobium crumenatum. Plant J 46, 54-68. Yang, Y., and Jack, T. (2004). Defining subdomains of the K domain important for protein-protein interactions of plant MADS proteins. Plant molecular biology 55, 45-59. Yang, Y., Fanning, L., and Jack, T. (2003a). The K domain mediates heterodimerization of the Arabidopsis floral organ identity proteins, APETALA3 and PISTILLATA. Plant J 33, 47-59. Yang, Y., Xiang, H., and Jack, T. (2003b). pistillata-5, an Arabidopsis B class mutant with strong defects in petal but not in stamen development. Plant J 33, 177-188. Yanofsky, M.F., Ma, H., Bowman, J.L., Drews, G.N., Feldmann, K.A., and Meyerowitz, E.M. (1990). The protein encoded by the Arabidopsis homeotic gene AGAMOUS resembles transcription factors. Nature 346, 35-39. Yu, D., Kotilainen, M., Pollanen, E., Mehto, M., Elomaa, P., Helariutta, Y., Albert, V.A., and Teeri, T.H. (1999). Organ identity genes and modified patterns of flower development in Gerbera hybrida (Asteraceae). Plant J 17, 51-62.
摘要: 
對植物來說,許多重要的生理發育過程是由MADS box基因所調控。而大部分影響花器形成的ABCDE基因皆屬於此類MADS box基因,其蛋白質結構中皆具有一段保守的DNA結合區域(MADS box domain),後有一段和蛋白質相互作用相關的K domain。為了能更清楚了解蘭科植物花器形成機制,本研究從文心蘭(Oncidium Gower Ramsey)中選殖出OMADS8及OMADS9兩個基因進行功能性分析。OMADS8可轉譯出一個含210個胺基酸的蛋白質,其序列比對結果發現,和阿拉伯芥B class中之PI基因有高度相似性,屬於PI同源性基因。OMADS8在文心蘭葉、根及花皆有表現。進一步觀察花器內部表現,發現OMADS8在花萼及花瓣表現量較雄蕊、心皮來的高。將OMADS8基因大量表現於野生型阿拉伯芥中,發現會使植株矮小提早開花,並造成花萼轉變成似花瓣的構造。OMADS9可轉譯出一個含222個胺基酸的蛋白質,其屬於B class中之PaleoAP3族基因,例如鴿石斛蘭(Dendrobium)的DcOAP3B和文心蘭的OMADS5皆屬於此類。OMADS9C端部分具有兩個完整的paleoAP3演化分支特有的motif — PI-derived motif及paleoAP3 motif。OMADS9在文心蘭花器中表現量以花瓣及唇辦中最高。大量表現OMADS9於阿拉伯芥,觀察到早開之花朵白化但較晚開的花和野生型無異。此外,透過酵母菌雙雜交系統(yeast two hybrid),來分析所知的文心蘭B群基因的蛋白質間的相互關係,結果顯示,OMADS5及OMADS9有形成homodimer的能力,也會相互作用形成 heterodimer。OMADS8無法形成同質二聚體,也無法形成異質二聚體。

MADS box genes controlled many important developmental processes in plants. Most ABCDE class genes regulating flower formation are MADS genes that contained a DNA-binding domain (MADS box domain) and a protein interaction domain (K domain) in their encoded proteins. To investigate the flower formation in orchid, two B class MADS box genes, OMADS8 and OMADS9 from Oncidium Gower Ramsey, were isolated and characterized in this research. OMADS8 encoded a 210 amino acid protein showed high sequence homology to Arabidopsis B class gene PI. OMADS8 is expressed in all the organs tested including leaf, root and flower. In flowers, OMADS8 mRNA was especially detected higher in the sepal and petal than in stamen and carpel. Ectopic expression of OMADS8 significantly reduced the plant size and promoted flowering time in transgenic Arabidopsis. The conversion of sepal to petal-like structure was also observed in 35S::OMADS8 transgenic plants. OMADS9 encoded a 222 amino acid protein and showed sequence homology to PaleoAP3 lineage of B group MADS box gene such as DcOAP3B in Dendrobium and OMADS5 in Oncidium. In its C-terminal region, two completely consensus motifs such as PI-derived and paleoAP3 motifs which are unique to paleoAP3 lineage were identified. OMADS9 is highly expressed in petal and lip petal in flowers. The early developed flowers are whitened, but the late developed flowers in transgenic Arabidopsis ectopically expressing OMADS9 are indistinguishable from that of wild-type plants. Furthermore, to investigate the interaction among the Oncidium B class proteins such as OMADS8, OMADS9 and OMADS5, yeast two hybrid analysis was performed. The result indicated that OMADS5 and OMADS9 were able to form homodimer and heterodimer to each other. OMADS8 was not able to form either homodimer or heterodimer.
URI: http://hdl.handle.net/11455/36140
其他識別: U0005-0608200713553700
Appears in Collections:生物科技學研究所

Show full item record
 

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.