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  2. 農業暨自然資源學院
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Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/36158
標題: 水稻結鈣激活酵素基因群之表現分布 及其僅於花粉大量表達成員之功能探討
Expression analysis of CDPK gene family and functional characterization of pollen-predominant CDPKs in rice
作者: 林忠威
Lin, Chung-Wei
關鍵字: pollen;花粉;CDPK gene family;水稻結鈣激活酵素基因群
出版社: 生物科技學研究所
引用: 李鐘財. (2000). 水稻花粉成熟期專一性表現基因OSCK1之選殖與分析.國立中興大學農業生物科技學系學研究所(現為生物科技學研究所)碩士論文. 陳婉潔. (2003). 水稻花粉結鈣激活酵素OSCK1之基因表現、蛋白胞內分部位置與基因轉殖植物分析. 國立中興大學生物科技學研究所碩士論文. 汪承偉. (2003). 利用酵母菌雙雜交法篩選可與OSCK1結合的水稻花粉蛋白. 國立中興大學生物科技學研究所碩士論文. 童嬿融. (2004). 第一部分：水稻OSCK基因靜默轉殖植物之分析；第二部分：水稻OIP基因表現模式之分析. 國立中興大學生物科技學研究所碩士論文. 王怡尹. (2005). 以轉基因植物分析水稻花粉結鈣激活酵素之功能.國立中興大學生物科技學研究所碩士論文. Abbasi, F., Onodera, H., Toki, S., Tanaka, H., and Komatsu, S. (2004). OsCDPK13, a calcium-dependent protein kinase gene from rice, is induced by cold and gibberellin in rice leaf sheath. Plant Mol Biol 55, 541-552. Akimoto-Tomiyama, C., Sakata, K., Yazaki, J., Nakamura, K., Fujii, F., Shimbo, K., Yamamoto, K., Sasaki, T., Kishimoto, N., Kikuchi, S., Shibuya, N., and Minami, E. (2003). Rice gene expression in response to N-acetylchitooligosaccharide elicitor: comprehensive analysis by DNA microarray with randomly selected ESTs. Plant Mol Biol 52, 537-551. Asano, T., Tanaka, N., Yang, G., Hayashi, N., and Komatsu, S. (2005). Genome-wide identification of the rice calcium-dependent protein kinase and its closely related kinase gene families: comprehensive analysis of the CDPKs gene family in rice. Plant cell physiol 46, 356-366. Asano, T., Kunieda, N., Omura, Y., Ibe, H., Kawasaki, T., Takano, M., Sato, M., Furuhashi, H., Mujin, T., Takaiwa, F., Wu Cy, C.Y., Tada, Y., Satozawa, T., Sakamoto, M., and Shimada, H. (2002). Rice SPK, a calmodulin-like domain protein kinase, is required for storage product accumulation during seed development: phosphorylation of sucrose synthase is a possible factor. Plant cell 14, 619-628. Bachvarov, D.R., and Ivanov, I.G. (1983). Large scale purification of plasmid DNA. Prep Biochem 13, 161-166. Balestrieri, C., Castaldo, D., Giovane, A., Quagliuolo, L., and Servillo, L. (1990). A glycoprotein inhibitor of pectin methylesterase in kiwi fruit (Actinidia chinensis). Eur J Biochem 193, 183-187. Barnabas, B., and Fridvalszky, L. (1984). Adhesion and germination of differently treated maize pollen grains on the stigma. Acta Biol Hung 30, 329–332. Birnboim, H.C., and Doly, J. (1979). A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7, 1513-1523. Chico, J.M., Raices, M., Tellez-Inon, M.T., and Ulloa, R.M. (2002). A calcium-dependent protein kinase is systemically induced upon wounding in tomato plants. Plant physiol 128, 256-270. Estruch, J.J., Kadwell, S., Merlin, E., and Crossland, L. (1994). Cloning and characterization of a maize pollen-specific calcium-dependent calmodulin-independent protein kinase. Proc Natl Acad Sci U S A 91, 8837-8841. Ferguson, C., Teeri, T.T., Siika, A.M., Read, S.M., and Bacic, A. (1998). Location of cellulose and callose in pollen tubes and grains of Nicotiana tabacum. Planta 206, 452–460. Gibbon, B.C., Kovar, D.R., and Staiger, C.J. (1999). Latrunculin B has different effects on pollen germination and tube growth. Plant cell 11, 2349-2363. Jeon, J.S., Chung, Y.Y., Lee, S., Yi, G.H., Oh, B.G., and An, G. (1999). Isolation and characterization of an anther-specific gene, RA8, from rice (Oryza sativa L.). Plant Mol Biol 39, 35-44. Jiang, L., Yang, S.L., Xie, L.F., Puah, C.S., Zhang, X.Q., Yang, W.C., Sundaresan, V., and Ye, D. (2005). VANGUARD1 encodes a pectin methylesterase that enhances pollen tube growth in the Arabidopsis style and transmitting tract. Plant cell 17, 584-596. Kawasaki, T., Hayashida, N., Baba, T., Shinozaki, K., and Shimada, H. (1993). The gene encoding a calcium-dependent protein kinase located near the sbe1 gene encoding starch branching enzyme I is specifically expressed in developing rice seeds. Gene 129, 183-189. Legnani, P., and Bianucci, F. (1983). [The use of the LB medium modified by preparation of Leishmania microcultures on glass slides]. Nuovi Ann Ig Microbiol 34, 491-495. Li, Y.Q., Faleri, C., Geitmann, A., Zhang, H.Q., and Cresti, M. (1995). Immunogold localization of arabinogalactan proteins, unesterified and esterified pectins in pollen grains and pollen tubes of Nicotiana tabacum L. Protoplasma 189, 26–36. Liu, G., Chen, J., and Wang, X. (2006). VfCPK1, a gene encoding calcium-dependent protein kinase from Vicia faba, is induced by drought and abscisic acid. Plant cell Environ 29, 2091-2099. Luo, H., Lee, J.Y., Hu, Q., Nelson-Vasilchik, K., Eitas, T.K., Lickwar, C., Kausch, A.P., Chandlee, J.M., and Hodges, T.K. (2006). RTS, a rice anther-specific gene is required for male fertility and its promoter sequence directs tissue-specific gene expression in different plant species. Plant Mol Biol 62, 397-408. Malho, R., and Trewavas, A.J. (1996). Localized Apical Increases of Cytosolic Free Calcium Control Pollen Tube Orientation. Plant cell 8, 1935-1949. Miller, D.D., de Ruijter, N.C.A., Bisseling, T., and Emons, A.M.C. (1999). The role of actin in root hair morphogenesis: studies with lipochitooligosaccharide as a growth stimulator and cytochalasin as an actn perturbing drug. Plant J 17, 141–154. Morello, L., Bardini, M., Cricri, M., Sala, F., and Breviario, D. (2006). Functional analysis of DNA sequences controlling the expression of the rice OsCDPK2 gene. Planta 223, 479-491. Nairn, C.J., Lewandowski, D.J., and Burns, J.K. (1998). Genetics and expression of two pectinesterase genes in Valencia orange. Plant Physiol 102, 226–235. Palanivelu, R., and Preuss, D. (2000). Pollen tube targeting and axon guidance: parallels in tip growth mechanisms. Trends Cell Biol 10, 517-524. Patharkar, O.R., and Cushman, J.C. (2000). A stress-induced calcium-dependent protein kinase from Mesembryanthemum crystallinum phosphorylates a two-component pseudo-response regulator. Plant J 24, 679-691. Romeis, T., Ludwig, A.A., Martin, R., and Jones, J.D. (2001). Calcium-dependent protein kinases play an essential role in a plant defence response. EMBO J 20, 5556-5567. Saijo, Y., Hata, S., Kyozuka, J., Shimamoto, K., and Izui, K. (2000). Over-expression of a single Ca2+-dependent protein kinase confers both cold and salt/drought tolerance on rice plants. Plant J 23, 319-327. Schenk, P.M., Kazan, K., Wilson, I., Anderson, J.P., Richmond, T., Somerville, S.C., and Manners, J.M. (2000). Coordinated plant defense responses in Arabidopsis revealed by microarray analysis. Proc Natl Acad Sci U S A 97, 11655-11660. Shimizu, K.K., and Okada, K. (2000). Attractive and repulsive interactions between female and male gametophytes in Arabidopsis pollen tube guidance. Development 127, 4511–4518. Taylor, L.P., and Hepler, P.K. (1997). Pollen Germination and Tube Growth. Annu Rev Plant Physiol Plant Mol Biol 48, 461-491. Tian, G.W., Chen, M.H., Zaltsman, A., and Citovsky, V. (2006). Pollen-specific pectin methylesterase involved in pollen tube growth. Dev Biol 294, 83-91. Wan, B., Lin, Y., and Mou, T. (2007). Expression of rice Ca(2+)-dependent protein kinases (CDPKs) genes under different environmental stresses. FEBS Lett 581, 1179-1189. Yang, G., Shen, S., Yang, S. and Komatsu, S. (2003). OsCDPK13, a calcium-dependent protein kinase gene from rice, is induced in response to cold and gibberellin. Plant Physiol Biochem 41: 369–374 Yoon, G.M., Dowd, P.E., Gilroy, S., and McCubbin, A.G. (2006). Calcium-dependent protein kinase isoforms in Petunia have distinct functions in pollen tube growth, including regulating polarity. Plant Cell 18, 867-878. Yu, X.C., Zhu, S.Y., Gao, G.F., Wang, X.J., Zhao, R., Zou, K.Q., Wang, X.F., Zhang, X.Y., Wu, F.Q., Peng, C.C., and Zhang, D.P. (2007). Expression of a grape calcium-dependent protein kinase ACPK1 in Arabidopsis thaliana promotes plant growth and confers abscisic acid-hypersensitivity in germination, postgermination growth, and stomatal movement. Plant Mol Biol 64, 531-538.
摘要: 
對於植物的有性生殖而言，花粉管能否於雌蕊上伸長是對植物授粉成功與否的關鍵步驟。當花粉通過自交不親合的篩選後，萌發的花粉管會穿透柱頭並沿著花柱朝胚珠生長。花粉中許多無機離子的濃度，例如鈣離子和鉀離子，對於花粉的萌發甚或是花粉管的生長扮演著相當重要的角色。Calcium-dependent protein kinases(CDPKs)即是植物界當中普遍存在的一種鈣離子接受者，因此可能多方影響花粉之生物功能。植物的CDPK參與了許多鈣離子所調控的訊息傳導途徑，常專一表現於特定的組織或發育時期，並受到特定外來訊息的調控。目前的研究指出水稻中的CDPK共有29個，是一多基因家族。為能有效利用RNAi實驗探討花粉中CDPK的功能，則必須先了解有何CDPK參與花粉萌發生長的調控，以專一性引子進行RT-PCR實驗發現，OsCPK2, OsCPK14, OsCPK21, OsCPK22, OsCPK25/26, OsCPK28及OsCPK29會在成熟花粉中大量，甚或是專一表現，由此大致確認，參與花粉萌發生長的CDPK基因共有八個。為觀察此八個CDPK於花粉萌發時可能之功能及參與的調控機制，構築會大量表現CDPK融合螢光蛋白(ECFP)的質體，利用基因槍進行短暫轉殖，使CDPK於花粉萌發時大量表現，並觀察花粉所受到之影響，藉此推測此八個CDPK於花粉萌發延長中可能扮演的角色，目前結果發現，四個水稻CDPK基因，OsCPK2, OsCPK21, OsCPK26及OsCPK29會抑制花粉管的生長。然而對推測可能分為兩群的CDPK基因而言，大量表現CDPK基因對花粉的影響並無明顯的區別。

Pollen tube elongation in pistil is a crucial step in the sexual reproduction of plants. In a compatible pollination, pollen germinates to extrude a tube that penetrates the stigma and grows extracellularly through the stylar tissues to ovules. It is known that pollen germination and tube growth are significantly regulated by the transport of inorganic ions, such as Ca2+ and K+, across the plasma membranes of pollen and/or pollen tubes. Calcium dependent protein kinase (CDPK) is one kind of the calcium receptors prevalent in plant kingdom, therefore which may play critical roles in multiple aspects of pollen function. The expressions of CDPKs are finely tuned in different tissues and in response to specific signals. Twenty-nine CDPK genes have been identified in the rice genome through a complete search of genome and full-length cDNA databases. To clarify how many CDPK genes participate in control of pollen tube growth, RT-PCR was performed in this study to detect the expression patterns of the 29 CDPK genes. Eight CDPKs (OsCPK2, OsCPK14, OsCPK21, OsCPK22, OsCPK25/26, OsCPK28 and OsCPK29) are expressed predominantly (or specifically) in the mature pollen. Using transient overexpression of CDPK/CFP fusion proteins in the mature pollen of lily, four of them (OsCPK2, OsCPK21, OsCPK26 and OsCPK29) showed inhibition of pollen tube elongation in a concentration-dependent manner. However, no clear functional differences were observed in the putative two clades of 8 CDPKs that expressed in the mature pollen.
URI: http://hdl.handle.net/11455/36158
其他識別: U0005-2808200711195300
Appears in Collections:生物科技學研究所

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