Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/36161
標題: 應用竹嵌紋病毒載體產製雞華氏囊病毒疫苗之 研究
Production of Infectious Bursal Disease Virus Vaccine by Bamboo Mosaic Virus-Based Vector
作者: 余思賢
Yu, Ssu-Shin
關鍵字: BaMV;竹嵌紋病毒;IBD;vector vaccine;雞華氏囊病;載體疫苗
出版社: 生物科技學研究所
引用: 林民昆 (2004)。竹嵌紋病毒三基因區蛋白之互補試驗及病毒載體之應用。國立 中興大學生物科技學研究所博士論文。 蔡向榮,呂榮修. 1993. 1992年台灣雞傳染性華氏囊病大流行之疫情分析.中華 獸醫誌 19:249-258. 蔡明成, 黃威霖, 柯冠銘, 張睦理, 張經緯與林茂勇. 2006. 台灣雞傳染性雞 華氏囊病抗體調查及其多種不活化疫苗在雞隻免疫反應. 台灣獸醫誌 32:30-39. 陳宗賢. 2005.應用竹嵌紋病毒載體產製日本腦炎疫苗之研究。國立中興大學生物 科技學研究所博士論文。 Ahlquist, P., R. French, M. Janda, and L. S. Loesch-Fries. 1984. Multicomponent RNA plant virus infection derived from cloned viral cDNA. Proc. Natl. Acad. Sci. U. S. A. 81:7066-7070. Avesani, L., G. Marconi, F. Morandini, E. Albertini, M. Bruschetta, L. Bortesi, M. Pezzotti, and A. Porceddu. 2007. Stability of Potato virus X expression vectors is related to insert size: implications for replication models and risk assessment. Transgenic Res. Beck, D. L., P. J. Guilford, D. M. Voot, M. T. Andersen, and R. L. Forster. 1991. Triple gene block proteins of white clover mosaic potexvirus are required for transport. Virology 183:695-702. Bottcher, B., N. A. Kiselev, V. Y. Stel''Mashchuk, N. A. Perevozchikova, A. V. Borisov, and R. A. Crowther. 1997. Three-dimensional structure of infectious bursal disease virus determined by electron cryomicroscopy. J. Virol. 71:325-330. Caston, J. R., J. L. Martinez-Torrecuadrada, A. Maraver, E. Lombardo, J. F. Rodriguez, J. I. Casal, and J. L. Carrascosa. 2001. C terminus of infectious bursal disease virus major capsid protein VP2 is involved in definition of the T number for capsid assembly. J. Virol. 75:10815-10828. Chang, B. Y., N. S. Lin, D. Y. Liou, J. P. Chen, G. G. Liou, and Y. H. Hsu. 1997. Subcellular localization of the 28 kDa protein of the triple-gene-block of bamboo 27 mosaic potexvirus. J. Gen. Virol. 78 ( Pt 5):1175-1179. Chen, C. S., S. Y. Suen, S. Y. Lai, G. R. Chang, T. C. Lu, M. S. Lee, and M. Y. Wang. 2005. Purification of capsid-like particles of infectious bursal disease virus (IBDV) VP2 expressed in E. coli with a metal-ion affinity membrane system. J. Virol. Methods 130:51-58. Chettle, N., J. C. Stuart, and P. J. Wyeth. 1989. Outbreak of virulent infectious bursal disease in East Anglia. Vet. Rec. 125:271-272. Chevalier, C., J. Lepault, B. Da Costa, and B. Delmas. 2004. The last C-terminal residue of VP3, glutamic acid 257, controls capsid assembly of infectious bursal disease virus. J. Virol. 78:3296-3303. Cho, Y. and S. A. Edgar. 1972. Characterization of infectious bursal disease. Poult. Sci. 51:60-69. Chung, B. N., T. Canto, and P. Palukaitis. 2007. Stability of recombinant plant viruses containing genes of unrelated plant viruses. J. Gen. Virol. 88:1347-1355. Coulibaly, F., C. Chevalier, I. Gutsche, J. Pous, J. Navaza, S. Bressanelli, B. Delmas, and F. A. Rey. 2005. The birnavirus crystal structure reveals structural relationships among icosahedral viruses. Cell 120:761-772. Cruz, S. S., S. Chapman, A. G. Roberts, I. M. Roberts, D. A. Prior, and K. J. Oparka. 1996. Assembly and movement of a plant virus carrying a green fluorescent protein overcoat. Proc. Natl. Acad. Sci. U. S. A. 93:6286-6290. Cruz-Revilla, C., A. M. Sonabend, G. Rosas, A. Toledo, G. Meneses, F. Lopez-Casillas, B. Hernandez, G. Fragoso, and E. Sciutto. 2006. Intrahepatic DNA vaccination: unexpected increased resistance against murine cysticercosis induced by non-specific enhanced immunity. J. Parasitol. 92:655-657. Cui, X., H. S. Nagesha, and I. H. Holmes. 2003. Identification of crucial residues of conformational epitopes on VP2 protein of infectious bursal disease virus by phage display. J. Virol. Methods 109:75-83. Cui, X., H. S. Nagesha, and I. H. Holmes. 2003. Mapping of conformational epitopes on capsid protein VP2 of infectious bursal disease virus by fd-tet phage display. J. Virol. Methods 114:109-112. Darteil, R., M. Bublot, E. Laplace, J. F. Bouquet, J. C. Audonnet, and M. Riviere. 28 1995. Herpesvirus of turkey recombinant viruses expressing infectious bursal disease virus (IBDV) VP2 immunogen induce protection against an IBDV virulent challenge in chickens. Virology 211:481-490. Dobos, P., B. J. Hill, R. Hallett, D. T. Kells, H. Becht, and D. Teninges. 1979. Biophysical and biochemical characterization of five animal viruses with bisegmented double-stranded RNA genomes. J. Virol. 32:593-605. Donnelly, M. L., D. Gani, M. Flint, S. Monaghan, and M. D. Ryan. 1997. The cleavage activities of aphthovirus and cardiovirus 2A proteins. J. Gen. Virol. 78 ( Pt 1):13-21. Donnelly, M. L., L. E. Hughes, G. Luke, H. Mendoza, E. ten Dam, D. Gani, and M. D. Ryan. 2001. The ''cleavage'' activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring ''2A-like'' sequences. J. Gen. Virol. 82:1027-1041. Eamens, G. J., J. C. Chin, B. Turner, and I. Barchia. 2006. Evaluation of Erysipelothrix rhusiopathiae vaccines in pigs by intradermal challenge and immune responses. Vet. Microbiol. 116:138-148. Eterradossi, N., J. P. Picault, P. Drouin, M. Guittet, R. L''Hospitalier, and G. Bennejean. 1992. Pathogenicity and preliminary antigenic characterization of six infectious bursal disease virus strains isolated in France from acute outbreaks. Zentralbl. Veterinarmed. B. 39:683-691. Fahey, K. J., K. Erny, and J. Crooks. 1989. A conformational immunogen on VP-2 of infectious bursal disease virus that induces virus-neutralizing antibodies that passively protect chickens. J. Gen. Virol. 70 ( Pt 6):1473-1481. Fernandez-Arias, A., S. Martinez, and J. F. Rodriguez. 1997. The major antigenic protein of infectious bursal disease virus, VP2, is an apoptotic inducer. J. Virol. 71:8014-8018. Francois, A., N. Eterradossi, B. Delmas, V. Payet, and P. Langlois. 2001. Construction of avian adenovirus CELO recombinants in cosmids. J. Virol. 75:5288-5301. Guittet, M., H. Le Coq, J. P. Picault, N. Eterradossi, and G. Bennejean. 1992. Safety of infectious bursal disease vaccines: assessment of an acceptability threshold. Dev. Biol. Stand. 79:147-152. 29 Hogarth, P. J., K. E. Logan, J. C. Ferraz, R. G. Hewinson, and M. A. Chambers. 2006. Protective efficacy induced by Mycobacterium bovis bacille Calmette-Guerin can be augmented in an antigen independent manner by use of non-coding plasmid DNA. Vaccine 24:95-101. Huang, Z., S. Elankumaran, A. S. Yunus, and S. K. Samal. 2004. A recombinant Newcastle disease virus (NDV) expressing VP2 protein of infectious bursal disease virus (IBDV) protects against NDV and IBDV. J. Virol. 78:10054-10063. Hudson, P. J., N. M. McKern, B. E. Power, and A. A. Azad. 1986. Genomic structure of the large RNA segment of infectious bursal disease virus. Nucleic Acids Res. 14:5001-5012. Ikuta, N., J. El-Attrache, P. Villegas, E. M. Garcia, V. R. Lunge, A. S. Fonseca, C. Oliveira, and E. K. Marques. 2001. Molecular characterization of Brazilian infectious bursal disease viruses. Avian Dis. 45:297-306. Inoue, M., H. Yamamoto, K. Matuo, and H. Hihara. 1992. Susceptibility of chicken monocytic cell lines to infectious bursal disease virus. J. Vet. Med. Sci. 54:575-577. Jackwood, D. J. and S. Sommer-Wagner. 2007. Genetic characteristics of infectious bursal disease viruses from four continents. (In Press) Virology Kibenge, F. S., A. S. Dhillon, and R. G. Russell. 1988. Biochemistry and immunology of infectious bursal disease virus. J. Gen. Virol. 69 ( Pt 8):1757-1775. Kim, S. J., H. W. Sung, J. H. Han, D. Jackwood, and H. M. Kwon. 2004. Protection against very virulent infectious bursal disease virus in chickens immunized with DNA vaccines. Vet. Microbiol. 101:39-51. Knoblich, H. V., S. E. Sommer, and D. J. Jackwood. 2000. Antibody titers to infectious bursal disease virus in broiler chicks after vaccination at one day of age with infectious bursal disease virus and Marek''s disease virus. Avian Dis. 44:874-884. Koudelka, K. J., C. S. Rae, M. J. Gonzalez, and M. Manchester. 2007. Interaction between a 54-kilodalton mammalian cell surface protein and cowpea mosaic virus. J. Virol. 81:1632-1640. Lee, C. C., T. P. Ko, C. C. Chou, M. Yoshimura, S. R. Doong, M. Y. Wang, and A. H. Wang. 2006. Crystal structure of infectious bursal disease virus VP2 subviral particle at 2.6A resolution: implications in virion assembly and immunogenicity. J. 30 Struct. Biol. 155:74-86. Lengler, J., H. Holzmuller, B. Salmons, W. H. Gunzburg, and M. Renner. 2005. FMDV-2A sequence and protein arrangement contribute to functionality of CYP2B1-reporter fusion protein. Anal. Biochem. 343:116-124. Leong, J.C., Brown, D., Dobos, P., Kibenge, F., Ludert, J.E., Müller, H., Mundt, E., Nicholson, B. 2000. Virus Taxonomy Classification and Nomenclature of Viruses. Academic Press, Li, J., X. Liang, Y. Huang, S. Meng, R. Xie, R. Deng, and L. Yu. 2004. Enhancement of the immunogenicity of DNA vaccine against infectious bursal disease virus by co-delivery with plasmid encoding chicken interleukin 2. Virology 329:89-100. Li, L., W. Fang, J. Li, Y. Huang, and L. Yu. 2006. Oral DNA vaccination with the polyprotein gene of infectious bursal disease virus (IBDV) delivered by the attenuated Salmonella elicits protective immune responses in chickens. Vaccine 24:5919-5927. Lico, C., F. Capuano, G. Renzone, M. Donini, C. Marusic, A. Scaloni, E. Benvenuto, and S. Baschieri. 2006. Peptide display on Potato virus X: molecular features of the coat protein-fused peptide affecting cell-to-cell and phloem movement of chimeric virus particles. J. Gen. Virol. 87:3103-3112. Lin, M. T., E. W. Kitajima, and Cupertino, F. P. and Costa, C. L. 1977. Partial purification and some properties of bamboo mosaic virus. Phytopathology 67:1439-1443. Lin, N. S. and Chen, C. C. 1991. Association of Bamboo mosaic virus (BaMV) and BaMV-specific electron dense crystalline bodies with chloroplasts. Phytopathology 81:1551-1555. Lin, N. S. and Y. H. Hsu. 1994. A satellite RNA associated with bamboo mosaic potexvirus. Virology 202:707-714. Lombardo, E., A. Maraver, n. J. R. Cast, J. Rivera, A. Fernandez-Arias, A. Serrano, J. L. Carrascosa, and J. F. Rodriguez. 1999. VP1, the putative RNA-dependent RNA polymerase of infectious bursal disease virus, forms complexes with the capsid protein VP3, leading to efficient encapsidation into virus-like particles. J. Virol. 73:6973-6983. Lu, Y. L. and H. K. Shieh. 1983. Infectious bursal disease In Taiwan. J. Chin. Soc. 31 Vet. Sci. 61-66. Lund, J. M., L. Alexopoulou, A. Sato, M. Karow, N. C. Adams, N. W. Gale, A. Iwasaki, and R. A. Flavell. 2004. Recognition of single-stranded RNA viruses by Toll-like receptor 7. Proc. Natl. Acad. Sci. U. S. A. 101:5598-5603. Mahmood, M. S., M. Siddique, I. Hussain, A. Khan, and M. K. Mansoor. 2006. Protection capability of recombinant plasmid DNA vaccine containing VP2 gene of very virulent infectious bursal disease virus in chickens adjuvanted with CpG oligodeoxynucleotide. Vaccine 24:4838-4846. Martinez-Torrecuadrada, J. L., N. Saubi, A. Pages-Mante, J. R. Caston, E. Espuna, and J. I. Casal. 2003. Structure-dependent efficacy of infectious bursal disease virus (IBDV) recombinant vaccines. Vaccine 21:3342-3350. Muller, H. and H. Becht. 1982. Biosynthesis of virus-specific proteins in cells infected with infectious bursal disease virus and their significance as structural elements for infectious virus and incomplete particles. J. Virol. 44:384-392. Muller, H. and R. Nitschke. 1987. The two segments of the infectious bursal disease virus genome are circularized by a 90,000-Da protein. Virology 159:174-177. Muller, H., C. Scholtissek, and H. Becht. 1979. The genome of infectious bursal disease virus consists of two segments of double-stranded RNA. J. Virol. 31:584-589. Muller, R., I. Kaufer, M. Reinacher, and E. Weiss. 1979. Immunofluorescent studies of early virus propagation after oral infection with infectious bursal disease virus (IBDV). Zentralbl. Veterinarmed. B. 26:345-352. Noe lle carette., Hans Engelkamp., Eric akpa., Sebastien J. Pierre., Neil R. Cameron., Peter c. M. Christianen., Jan C. Maan., Jens C. Thies., Ralf Weberskirch., Alan E. Rowan., Roeland J. M. Nolte., Thierry Michon . and Jan C. M. Van Hest. 2007. A virus-based biocatalyst. nnano 2:226-229. Nunoya, T., Y. Otaki, M. Tajima, M. Hiraga, and T. Saito. 1992. Occurrence of acute infectious bursal disease with high mortality in Japan and pathogenicity of field isolates in specific-pathogen-free chickens. Avian Dis. 36:597-609. Okoye, J. O. and M. Uzoukwu. 1981. An outbreak of infectious bursal disease among chickens between 16 and 20 weeks old. Avian Dis. 25:1034-1038. Oppling, V., H. Muller, and H. Becht. 1991. Heterogeneity of the antigenic site 32 responsible for the induction of neutralizing antibodies in infectious bursal disease virus. Arch. Virol. 119:211-223. Oppling, V., H. Muller, and H. Becht. 1991. The structural polypeptide VP3 of infectious bursal disease virus carries group- and serotype-specific epitopes. J. Gen. Virol. 72 ( Pt 9):2275-2278. Osborn, M. J., A. Panoskaltsis-Mortari, R. T. McElmurry, S. K. Bell, D. A. Vignali, M. D. Ryan, A. C. Wilber, R. S. McIvor, J. Tolar, and B. R. Blazar. 2005. A picornaviral 2A-like sequence-based tricistronic vector allowing for high-level therapeutic gene expression coupled to a dual-reporter system. Mol. Ther. 12:569-574. Parker, L., A. Kendall, and G. Stubbs. 2002. Surface features of potato virus X from fiber diffraction. Virology 300:291-295. Phenix, K. V., K. Wark, C. J. Luke, M. A. Skinner, J. A. Smyth, K. A. Mawhinney, and D. Todd. 2001. Recombinant Semliki Forest virus vector exhibits potential for avian virus vaccine development. Vaccine 19:3116-3123. Porta, C., V. E. Spall, J. Loveland, J. E. Johnson, P. J. Barker, and G. P. Lomonossoff. 1994. Development of cowpea mosaic virus as a high-yielding system for the presentation of foreign peptides. Virology 202:949-955. Rodenberg, J., J. M. Sharma, S. W. Belzer, R. M. Nordgren, and S. Naqi. 1994. Flow cytometric analysis of B cell and T cell subpopulations in specific-pathogen-free chickens infected with infectious bursal disease virus. Avian Dis. 38:16-21. Rodriguez-Lecompte, J. C., R. Nino-Fong, A. Lopez, R. J. Frederick Markham, and F. S. Kibenge. 2005. Infectious bursal disease virus (IBDV) induces apoptosis in chicken B cells. Comp. Immunol. Microbiol. Infect. Dis. 28:321-337. Ryan, P. C., M. Lu, and D. E. Draper. 1991. Recognition of the highly conserved GTPase center of 23 S ribosomal RNA by ribosomal protein L11 and the antibiotic thiostrepton. J. Mol. Biol. 221:1257-1268. Sharma, J. M. 1999. Introduction to poultry vaccines and immunity. Adv. Vet. Med. 41:481-494. Sharma, J. M., Y. Zhang, D. Jensen, S. Rautenschlein, and H. Y. Yeh. 2002. Field trial in commercial broilers with a multivalent in ovo vaccine comprising a mixture of live viral vaccines against Marek''s disease, infectious bursal disease, Newcastle 33 disease, and fowl pox. Avian Dis. 46:613-622. Shaw, I. and T. F. Davison. 2000. Protection from IBDV-induced bursal damage by a recombinant fowlpox vaccine, fpIBD1, is dependent on the titre of challenge virus and chicken genotype. Vaccine 18:3230-3241. Siegel A. 1983. RNA viruses as cloning vehicles. Phytopathology 1983:775. Tacken, M. G., P. J. Rottier, A. L. Gielkens, and B. P. Peeters. 2000. Interactions in vivo between the proteins of infectious bursal disease virus: capsid protein VP3 interacts with the RNA-dependent RNA polymerase, VP1. J. Gen. Virol. 81:209-218. Tsukamoto, K., C. Kojima, Y. Komori, N. Tanimura, M. Mase, and S. Yamaguchi. 1999. Protection of chickens against very virulent infectious bursal disease virus (IBDV) and Marek''s disease virus (MDV) with a recombinant MDV expressing IBDV VP2. Virology 257:352-362. Tsukamoto, K., T. Sato, S. Saito, N. Tanimura, N. Hamazaki, M. Mase, and S. Yamaguchi. 2000. Dual-viral vector approach induced strong and long-lasting protective immunity against very virulent infectious bursal disease virus. Virology 269:257-267. Tsukamoto, K., N. Tanimura, S. Kakita, K. Ota, M. Mase, K. Imai, and H. Hihara. 1995. Efficacy of three live vaccines against highly virulent infectious bursal disease virus in chickens with or without maternal antibodies. Avian Dis. 39:218-229. Uhde-Holzem, K., R. Fischer, and U. Commandeur. 2007. Genetic stability of recombinant potato virus X virus vectors presenting foreign epitopes. Arch. Virol. 152:805-811. Wu, H., N. K. Singh, R. D. Locy, K. Scissum-Gunn, and J. J. Giambrone. 2004. Immunization of chickens with VP2 protein of infectious bursal disease virus expressed in Arabidopsis thaliana. Avian Dis. 48:663-668. Wu, P. C., H. Y. Su, L. H. Lee, D. T. Lin, P. C. Yen, and H. J. Liu. 2005. Secreted expression of the VP2 protein of very virulent infectious bursal disease virus in the methylotrophic yeast Pichia pastoris. J. Virol. Methods 123:221-225. Wung, C. H., Y. H. Hsu, D. Y. Liou, W. C. Huang, and Lin, N. S. and Chang, B. Y. 1999. Identification of the RNA-binding sites of the triple gene block protein 1 of bamboo mosaic potexvirus. J. Gen. Virol 80:1119-1126. 34 Yamaguchi, T., K. Iwata, M. Kobayashi, M. Ogawa, H. Fukushi, and K. Hirai. 1996. Epitope mapping of capsid proteins VP2 and VP3 of infectious bursal disease virus. Arch. Virol. 141:1493-1507. Yang, C. C., J. S. Liu, and Lin, C.P. and Lin, N. S. 1997. Nucleotide sequence and phylogenetic analysis of bamboo mosaic potexvirus isolate from common bamboo (Bambusa vulgaris McClure). Bot. Bull. Acad. Sin. 38:77-84. Yeh, C. C. and Cheng, A. H. and Hwang, H. Y. 1993. Indexing of bamboo mosaic virus and propagation of virus-free bamboo. In R. C. Chiu and Y. Yeh (ed), Proceedings of the Symposium on Plant Virus and Viruslike Diseases. Plant Protection 1:275-282. Yip, C. W., Y. S. Yeung, C. M. Ma, P. Y. Lam, C. C. Hon, F. Zeng, and F. C. Leung. 2007. Demonstration of receptor binding properties of VP2 of very virulent strain infectious bursal disease virus on Vero cells. Virus Res. 123:50-56. 35
摘要: 
雞傳染性華氏囊病毒(Infectious bursal disease virus, IBDV)為造成雞華氏囊
病之主因,本研究嘗試利用生產成本較低廉的植物病毒-竹嵌紋病毒(Bamboo
mosaic virus, BaMV)載體生產vvIBDV抗原。由於IBDV的VP2蛋白之P區域
(P-domain)可誘導雞隻產生出中和性抗體,因此本實驗將VP2蛋白的P-domain或
將P-domain分成多段胜肽作為抗原分別融合在BaMV外鞘蛋白的N端,利用重組
病毒感染,在植物體內大量表現胜肽抗原嵌合病毒。此外,在融合整段P-domain
的實驗中,我們在BaMV外鞘蛋白和P-domain中間插入口蹄疫病毒的2A序列以達
到部分自動斷裂的效果。利用西方墨點法偵測融合胜肽抗原之鞘蛋白在植物體內
的表現量後,挑選三株竹嵌紋病毒重組株,以純化的病毒當抗原進行雞隻免疫,
觀察其免疫後的反應及保護力,其中兩株有明顯的抗體反應及免疫保護力保護的
能力。口蹄疫病毒2A序列插入的竹嵌紋重組病毒株,雖能表現vvIBDV VP2 P
-domain,但是由於2A序列切割活性太強,竹嵌紋病毒外鞘蛋白和P區域的融和
蛋白比例太低,未來必須重新調整2A序列的切割活性。
3

Infectious bursal disease virus, IBDV, is the major cause for infectious bursal disease
of chickens. Two strategies are applied in our experiments, fusing either short
peptides or the intact P-domain of VP2 with the N-terminal of BaMV coat protein as
antigens. The stability and yield of chimeric virus particles loaded with antigens are
examined by inoculating these chimeric BaMV clones into plants. In order to
facilitate the assembly of chimeric BaMV with the intact P-domain, we inserted a
FMDV 2A sequence to bridge the BaMV coat protein from the vvIBDV P-domain
sequence. The active auto-cleavage of the 2A sequence renders the assembled
chimeric virus particles with only a small amount of fusion protein. After careful
evaluation of the recombinant proteins by using western blot analyses, we selected
three BaMV chimeric clones with peptide fusions and inoculated plants, then purified
the virus particles as antigens for animal tests. In our preliminary results, two of the
three chimeric BaMV vaccines showed moderate protection ability. The yields of
chimeric BaMV particles containing the intact P-domain in plants were low; most of
the P-domain was cleaved. Further adjustment of 2A cleavage activity is needed.
URI: http://hdl.handle.net/11455/36161
其他識別: U0005-3107200717342900
Appears in Collections:生物科技學研究所

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