Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/36184
標題: 竹嵌紋病毒衛星核酸蛋白P20與粒線體關係之研究
Association of Bamboo mosaic virus satellite RNA encoded P20 with mitochondria
作者: 王冠智
Wang, Guan-Zhi
關鍵字: satBaMV;竹嵌紋病毒;P20;mitochondria;confocal;衛星核酸;粒線體
出版社: 生物科技學研究所
引用: 柒、參考文獻 廖家德。2000。竹嵌紋病毒病徵表現之遺傳分析及載體構築。國立中興大學農業生物科技學研究所博士論文。 陳 薇。2003。竹嵌紋病毒衛星核酸轉譯蛋白質 P20之生物功能及其對衛星核酸活性之影響。國立中興大學農業生物科技學研究所博士論文。 李雅倩。2005。發展轉殖植物表現竹嵌紋病毒及其衛星核酸並應用其於輔助病毒-衛星核酸交互作用之研究。國立中興大學生物科技學研究所博士論文。 Abramov, A. Y., Fraley, C., Diao, C. T., Winkfein, R., Colicos, M. A., Duchen, M. R., French, R. J., and Pavlov, E. (2007). Targeted polyphosphatase expression alters mitochondrial metabolism and inhibits calcium-dependent cell death. Proc Natl Acad Sci U S A 104(46), 18091-6. Ahting, U., Waizenegger, T., Neupert, W., and Rapaport, D. (2005). Signal-anchored proteins follow a unique insertion pathway into the outer membrane of mitochondria. J Biol Chem 280(1), 48-53. Angell, S. M., Davies, C., and Baulcombe, D. C. (1996). Cell-to-cell movement of potato virus X is associated with a change in the size-exclusion limit of plasmodesmata in trichome cells of Nicotiana clevelandii. Virology 216(1), 197-201. Annamalai, P., Hsu, Y. H., Liu, Y. P., Tsai, C. H., and Lin, N. S. (2003). Structural and mutational analyses of cis-acting sequences in the 5''-untranslated region of satellite RNA of bamboo mosaic potexvirus. Virology 311(1), 229-39. Arimura, S., Yamamoto, J., Aida, G. P., Nakazono, M., and Tsutsumi, N. (2004). Frequent fusion and fission of plant mitochondria with unequal nucleoid distribution. Proc Natl Acad Sci U S A 101(20), 7805-8. Beatch, M. D., and Hobman, T. C. (2000). Rubella virus capsid associates with host cell protein p32 and localizes to mitochondria. J Virol 74(12), 5569-76. Beck, D. L., Guilford, P.J., Voot, D.M., Andersen, M.T., and Forster, R.L.S. (1991). Triple gene block proteins of white clover mosaic potexvirus are required for transport. Virology 183(2), 695-702. Behrens, M., Michaelis, G., and Pratje, E. (1991). Mitochondrial inner membrane protease 1 of Saccharomyces cerevisiae shows sequence similarity to the Escherichia coli leader peptidase. Mol Gen Genet 228(1-2), 167-76. Brooks, C., Wei, Q., Feng, L., Dong, G., Tao, Y., Mei, L., Xie, Z. J., and Dong, Z. (2007). Bak regulates mitochondrial morphology and pathology during apoptosis by interacting with mitofusins. Proc Natl Acad Sci U S A 104(28), 11649-54. Bruce, A., Alexander, J., Julian, L., Martin, R., Keith, R., and Peter, W. (2002). Molecular Biology of the Cell, 4th Edition. Buck, K. W. (1996). Comparison of the replication of positive-stranded RNA viruses of plants and animals. Adv Virus Res 47, 159-251. Chen, H., McCaffery, J. M., and Chan, D. C. (2007). Mitochondrial fusion protects against neurodegeneration in the cerebellum. Cell 130(3), 548-62. Chen, S., and Dickman, M. B. (2004). Bcl-2 family members localize to tobacco chloroplasts and inhibit programmed cell death induced by chloroplast-targeted herbicides. J Exp Bot 55(408), 2617-23. Curtis, M. J., and Wolpert, T. J. (2002). The oat mitochondrial permeability transition and its implication in victorin binding and induced cell death. Plant J 29(3), 295-312. Dagan, A., Agmon, V., Gatt, S., and Dinur, T. (2000). Synthesis of fluorescent substrates and their application to study of sphingolipid metabolism in vitro and in intact cells. Methods Enzymol 312, 293-304. David, L. N., and Michanel, M. C. (2004). Lehninger Principles of Biochemistry. 4th Edition. Forster, R. L., Beck, D. L., Guilford, P. J., Voot, D. M., Van Dolleweerd, C. J., and Andersen, M. T. (1992). The coat protein of white clover mosaic potexvirus has a role in facilitating cell-to-cell transport in plants. Virology 191(1), 480-4. Gross, T., and Rees, T. (1986). Alkaline inorganic pyrophosphatase and starch synthesis in amyloplasts. Planta 167, 140-45. Guo, Y. X., Chan, S. W., and Kwang, J. (2004). Membrane association of greasy grouper nervous necrosis virus protein A and characterization of its mitochondrial localization targeting signal. J Virol 78(12), 6498-508. Harrison, B. D., and Roberts, I. M. (1968). Association of tobacco rattle virus with mitochondria. J Gen Virol 3(1), 121-4. Harrison, B. D., Stefanac, Z., and Roberts, I. M. (1970). Role of mitochondria in the formation of X-bodies in cells of Nicotiana clevelandii infected by Tobacco rattle virus. J Gen Virol 6, 127-40. Herrmann, J. M., and Neupert, W. (2000). Protein transport into mitochondria. Curr Opin Microbiol 3(2), 210-4. Hsu, Y. H., Lee, Y. S., Liu, J. S., and Lin, N. S. (1998). Differential Interactions of Bamboo Mosaic Potexvirus Satellite RNAs, Helper Virus, and Host Plants. MPMI 11(12), 1207–13. Kim, M., Lim, J. H.,Ahn, C. S.,Park, K.,Kim, G. T.,Kim, W. T., and Pai, H. S. (2006). Mitochondria-associated hexokinases play a role in the control of programmed cell death in Nicotiana benthamiana. Plant Cell 18(9), 2341-55. Kristensen, B. K., Askerlund, P., Bykova, N. V., Egsgaard, H., and Moller, I. M. (2004). Identification of oxidised proteins in the matrix of rice leaf mitochondria by immunoprecipitation and two-dimensional liquid chromatography-tandem mass spectrometry. Phytochemistry 65(12), 1839-51. Lee, C. M., Sedman, J., Neupert, W., and Stuart, R. A. (1999). The DNA helicase, Hmi1p, is transported into mitochondria by a C-terminal cleavable targeting signal. J Biol Chem 274(30), 20937-42. Lee, C. P., Eubel, H., O''Toole, N., and Millar, A. H. (2008). Heterogeneity of the mitochondrial proteome for photosynthetic and non-photosynthetic Arabidopsis metabolism. Mol Cell Proteomics. Lee, W. M., Ishikawa, M., and Ahlquist, P. (2001). Mutation of host delta9 fatty acid desaturase inhibits brome mosaic virus RNA replication between template recognition and RNA synthesis. J Virol 75(5), 2097-106. Li, Y. I., Chen, Y. J., Hsu, Y. H., and Meng, M. (2001a). Characterization of the AdoMet-dependent guanylyltransferase activity that is associated with the N terminus of bamboo mosaic virus replicase. J Virol 75(2), 782-8. Li, Y. I., Cheng, Y. M., Huang, Y. L., Tsai, C. H., Hsu, Y. H., and Meng, M. (1998). Identification and characterization of the Escherichia coli-expressed RNA-dependent RNA polymerase of bamboo mosaic virus. J Virol 72(12), 10093-9. Li, Y. I., Shih, T. W., Hsu, Y. H., Han, Y. T., Huang, Y. L., and Meng, M. (2001b). The helicase-like domain of plant potexvirus replicase participates in formation of RNA 5'' cap structure by exhibiting RNA 5''-triphosphatase activity. J Virol 75(24), 12114-20. Lin, J. W., Ding, M. P., Hsu, Y. H., and Tsai, C. H. (2007). Chloroplast phosphoglycerate kinase, a gluconeogenetic enzyme, is required for efficient accumulation of Bamboo mosaic virus. Nucleic Acids Res 35(2), 424-32. Lin, M. K., Chang, B. Y., Liao, J. T., Lin, N. S., and Hsu, Y. H. (2004). Arg-16 and Arg-21 in the N-terminal region of the triple-gene-block protein 1 of Bamboo mosaic virus are essential for virus movement. J Gen Virol 85(Pt 1), 251-9. Lin, M. K., Hu, C. C., Lin, N. S., Chang, B. Y., and Hsu, Y. H. (2006). Movement of potexviruses requires species-specific interactions among the cognate triple gene block proteins, as revealed by a trans-complementation assay based on the bamboo mosaic virus satellite RNA-mediated expression system. J Gen Virol 87(Pt 5), 1357-67. Lin, M. T., Kitajima, E. W., Cupertino, F. P., and L., C. C. (1977). Partial purification and some properties of bamboo mosaic virus. Phytopathology 67, 1439-43. Lin, N. S., and Hsu, Y. H. (1994). A satellite RNA associated with bamboo mosaic potexvirus. Virology 202(2), 707-14. Lin, N. S., Lee, Y. S., Lin, B. Y., Lee, C. W., and Hsu, Y. H. (1996). The open reading frame of bamboo mosaic potexvirus satellite RNA is not essential for its replication and can be replaced with a bacterial gene. Proc Natl Acad Sci U S A 93(7), 3138-42. Lin, N. S., Lin, B. Y., Lo, N. W., Hu, C. C., Chow, T. Y., and Hsu, Y. H. (1994). Nucleotide sequence of the genomic RNA of bamboo mosaic potexvirus. J Gen Virol 75 ( Pt 9), 2513-8. Lin, N. S., Lin, F. Z., Huang, T. Y., and Hsu, Y. H. (1992). Genome proterties of bamboo mosaic virus. Phytopathology 82, 731-4. Liu, J. S., Hsu, Y. H., Huang, T. Y., and Lin, N. S. (1997). Molecular evolution and phylogeny of satellite RNA associated with bamboo mosaic potexvirus. J Mol Evol 44(2), 207-13. Liu, J. S., and Lin, N. S. (1995). Satellite RNA associated with bamboo mosaic potexvirus shares similarity with satellites associated with sobemoviruses. Arch Virol 140(8), 1511-4. Lo, Y. S., Hsiao, L. J., Jane, W. N., Charng, Y. C., Dai, H., and Chiangy, K. S. (2004). GFP-targeted mitochondria show heterogeneity of size, morphology, and dynamics in transgenic nicotiana tabacuml. plant in vivo. Int. J. Plant Sci. 165(6), 949-55. Logan, D. C., and Leaver, C. J. (2000). Mitochondria-targeted GFP highlights the heterogeneity of mitochondrial shape, size and movement within living plant cells. J Exp Bot 51(346), 865-71. Lough, T. J., Shash, K., Xoconostle-Cazares, B., R., H. K., David, L., Balmori, E., Beck, D. L., Forster, R. L. S., and J., L. W. (1998). Molecular Dissection of the Mechanism by Which Potexvirus Triple Gene Block Proteins Mediate Cell-to-Cell Transport of Infectious RNA. Mol. Plant-Microbe. Interact. 11, 801-14. Macasev, D., Newbigin, E., Whelan, J., and Lithgow, T. (2000). How do plant mitochondria avoid importing chloroplast proteins? Components of the import apparatus Tom20 and Tom22 from Arabidopsis differ from their fungal counterparts. Plant Physiol 123(3), 811-6. Malcuit, I., Marano, M. R., Kavanagh, T. A., D., J. W., Forsyth, A., and Baulcombe, D. C. (1999). The 25-kDa movement protein of PVX elicits Nb-mediated hypersensitive cell death in potato. Mol. Plant-Micro Interact. 12, 536-543. Mas, P., and Beachy, R. N. (1999). Replication of tobacco mosaic virus on endoplasmic reticulum and role of the cytoskeleton and virus movement protein in intracellular distribution of viral RNA. J Cell Biol 147(5), 945-58. Millar, A. H., Sweetlove, L. J., Giege, P., and Leaver, C. J. (2001). Analysis of the Arabidopsis mitochondrial proteome. Plant Physiol 127(4), 1711-27. Miller, D. J., and Ahlquist, P. (2002). Flock house virus RNA polymerase is a transmembrane protein with amino-terminal sequences sufficient for mitochondrial localization and membrane insertion. J Virol 76(19), 9856-67. Mokranjac, D., and Neupert, W. (2005). Protein import into mitochondria. Biochem Soc Trans 33(Pt 5), 1019-23. Neuberger, M. (1985). Preparation of plant mitochondria, criteria for assessment of mitochondrial integrity and purity, survival in vitro. In R Douce, D Day, eds, Higher Plant Cell Respiration. Springer-Verlag, Berlin., 7–24. Pagano, R. E., Watanabe, R., Wheatley, C., and Dominguez, M. (2000). Applications of BODIPY-sphingolipid analogs to study lipid traffic and metabolism in cells. Methods Enzymol 312, 523-34. Palatnik, J. F., Valle, E. M., and Carrillo, N. (1997). Oxidative stress causes ferredoxin-NADP+ reductase solubilization from the thylakoid membranes in methyl viologen-treated plants. Plant Physiol 115(4), 1721-7. Preuss, M., Ott, M., Funes, S., Luirink, J., and Herrmann, J. M. (2005). Evolution of mitochondrial oxa proteins from bacterial YidC. Inherited and acquired functions of a conserved protein insertion machinery. J Biol Chem 280(13), 13004-11. Prod''homme, D., Le Panse, S., Drugeon, G., and Jupin, I. (2001). Detection and subcellular localization of the turnip yellow mosaic virus 66K replication protein in infected cells. Virology 281(1), 88-101. Rokov-Plavec, J., Dulic, M., Duchene, A. M., and Weygand-Durasevic, I. (2008). Dual targeting of organellar seryl-tRNA synthetase to maize mitochondria and chloroplasts. Plant Cell Rep 27(7), 1157-68. Rubino, L., Di Franco, A., and Russo, M. (2000). Expression of a plant virus non-structural protein in Saccharomyces cerevisiae causes membrane proliferation and altered mitochondrial morphology. J Gen Virol 81(Pt 1), 279-86. Rubino, L., and Russo, M. (1998). Membrane targeting sequences in tombusvirus infections. Virology 252(2), 431-7. Santa Cruz, R., A. G., Prior, D. A., Chapman, S., Oparka, K. J. (1998). Cell-to-cell and phloem-mediated transport of potato virus X. The role of virions. Plant Cell 10(4), 495-510. Schaad, M. C., Jensen, P. E., and Carrington, J. C. (1997). Formation of plant RNA virus replication complexes on membranes: role of an endoplasmic reticulum-targeted viral protein. EMBO J 16(13), 4049-59. Schneider, A. (1991). Mitochondrial inner membrane protease I of saccharomyces cerevisiae. Methods Cell Biol 34, 401-7. Schwer, B., Ren, S., Pietschmann, T., Kartenbeck, J., Kaehlcke, K., Bartenschlager, R., Yen, T. S., and Ott, M. (2004). Targeting of hepatitis C virus core protein to mitochondria through a novel C-terminal localization motif. J Virol 78(15), 7958-68. Smith, A., and Rees, T. (1979). Pathways of carbohydrate fermentation in the roots of marsh plants. Planta 146, 327-334. Tomas, H., David, H., and VeraC, a. (2004). New method of plant mitochondria isolation and sub-fractionation for proteomic analyses. Plant Science 167 389-395. Truscott, K. N., Brandner, K., and Pfanner, N. (2003). Mechanisms of protein import into mitochondria. Curr Biol 13(8), R326-37. Tsai, C. H., Cheng, C. P., Peng, C. W., Lin, B. Y., Lin, N. S., and Hsu, Y. H. (1999). Sufficient length of a poly(A) tail for the formation of a potential pseudoknot is required for efficient replication of bamboo mosaic potexvirus RNA. J Virol 73(4), 2703-9. Tsai, M. S., Hsu, Y. H., and Lin, N. S. (1999). Bamboo mosaic potexvirus satellite RNA (satBaMV RNA)-encoded P20 protein preferentially binds to satBaMV RNA. J Virol 73(4), 3032-9. Valentin, C., Dunoyer, P., Vetter, G., Schalk, C., Dietrich, A., and Bouzoubaa, S. (2005). Molecular basis for mitochondrial localization of viral particles during beet necrotic yellow vein virus infection. J Virol 79(15), 9991-10002. Van Der Heijden, M. W., Carette, J. E., Reinhoud, P. J., Haegi, A., and Bol, J. F. (2001). Alfalfa mosaic virus replicase proteins P1 and P2 interact and colocalize at the vacuolar membrane. J Virol 75(4), 1879-87. Vijaya Palani, P., Kasiviswanathan, V., Chen, J. C., Chen, W., Hsu, Y. H., and Lin, N. S. (2006). The arginine-rich motif of Bamboo mosaic virus satellite RNA-encoded P20 mediates self-interaction, intracellular targeting, and cell-to-cell movement. Mol Plant Microbe Interact 19(7), 758-67. Weber-Lotfi, F., Dietrich, A., Russo, M., and Rubino, L. (2002). Mitochondrial targeting and membrane anchoring of a viral replicase in plant and yeast cells. J Virol 76(20), 10485-96. Wischmann, C., and Schuster, W. (1995). Transfer of rps10 from the mitochondrion to the nucleus in Arabidopsis thaliana: evidence for RNA-mediated transfer and exon shuffling at the integration site. FEBS Lett 374(2), 152-6. Wung, C. H., Hsu, Y. H., Liou, D. Y., Huang, W. C., Lin, N. S., and Chang, B. Y. (1999). Identification of the RNA-binding sites of the triple gene block protein 1 of bamboo mosaic potexvirus. J Gen Virol 80 ( Pt 5), 1119-26. Yang, C. C., Liu, J. S., Lin, C. P., and Lin, N. S. (1997). Nucleotide sequence and phylogenetic analysis of a bamboo mosaic potexvirus isolate from common bamboo (Bambusa vulgaris McClure). Bot. Bull. Acad. Sin. 38, 77-84. Yang, Y., Ouyang, Y., Yang, L., Beal, M. F., McQuibban, A., Vogel, H., and Lu, B. (2008). Pink1 regulates mitochondrial dynamics through interaction with the fission/fusion machinery. Proc Natl Acad Sci U S A 105(19), 7070-5. Yeh, W. B., Hsu, Y. H., Chen, H. C., and Lin, N. S. (2004). A conserved secondary structure in the hypervariable region at the 5'' end of Bamboo mosaic virus satellite RNA is functionally interchangeable. Virology 330(1), 105-15. Yoshinaga, K., Arimura, S., Niwa, Y., Tsutsumi, N., Uchimiya, H., and Kawai-Yamada, M. (2005). Mitochondrial behaviour in the early stages of ROS stress leading to cell death in Arabidopsis thaliana. Ann Bot (Lond) 96(2), 337-42.
摘要: 
壹、中文摘要

竹嵌紋病毒 (Bamboo mosaic virus, BaMV),為彎曲絲狀外型,單股正極性的RNA病毒。有些病毒分離株攜帶有衛星核酸 (satellite RNA, satBaMV),其複製與包被必須完全依賴BaMV的協助。satBaMV RNA全長為836個核

Bamboo mosaic virus (BaMV), is a single-stranded positive-sense RNA virus with flexuous rod-shaped morphology. The satellite RNA (full-length 836 nt) associated with BaMV, designated as satBaMV RNA, encodes a 20 kDa nonstructural protein (P20). Using several programs to predict the subcellular locatizaion of P20, we found transmembrane domain and mitochondrial targeting signal (MTS), typical viral proteins that can localize on mitochondria. We used discontinuous percoll gradient to isolate mitochondria of Nicotiana benthamiana. The mitochondrial fraction was confirmed by a mitochondrial specific dye Mitotracker Orange CMTMRos and antibody of cytochrome c. Using western blot analysis P20 was detected in mitochondrial enriched fraction from satBaMV transgenic line 2-6 that was inoculated with BaMV. Protoplasts were prepared from inoculated leaves of N. benthamiana co-infected with BaMV-S viral RNA and in vitro transcripts of pBSGFP, pBSGFPP20 and pBSP20GFP, respectively. By Confocal microscopy, protoplasts from pBSGFPP20 infected leave, similar to pBSGFP displayed the green fluorescence which scattered in cytoplasm. The protoplasts contained P20GFP had obvious GFP signal that co-localized and surrounded with mitochondria of diameter at least 2μm at low expression, but the fluorescence were too strong to recognize if P20GFP had transported into mitochondria. When P20GFP gradual accumulated, the phenomenon which green fluorescence co-localize with mitochondria was lost and the 2μm diameter mitochondria were disappeared. The Protease K protection assay showed the mitochondrial membrane could be preserved completely 7 days after isolation. However P20 within mitochondrial fraction from N. benthamiana line 2-6 challenged with BaMV-S could not be protected by mitochondrial membrane after Protease K treatment. No appearance of products once MTS predicted by MitoProt II were cleaved as most of mitochondrial import mechanism. Our data support a hypothesis that P20 of satBaMV interacted with mitochondria that may be just at early phase of
viral life cycle.
URI: http://hdl.handle.net/11455/36184
其他識別: U0005-2307200816284700
Appears in Collections:生物科技學研究所

Show full item record
 

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.