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標題: 研究傳染性華氏囊病毒次病毒顆粒VP2蛋白 表面胺基酸His249與His253對鎳離子 之親合性及免疫原性的影響
Investigation of the roles of two surface residues His249&His253 on the affinity to the immobilized Ni2+ ions and immunogenicity of infectious bursal disease virus VP2 subviral particles
作者: 楊函蓁
Yang, Han-Jen
關鍵字: infectious bursal disease virus;傳染性華氏囊病毒;Ni ions;immunogenicity;鎳離子;免疫原性
出版社: 生物科技學研究所
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本實驗室先前研究中證實利用固定化金屬親合性管柱 (Immobilized metal ion affinity chromatography, IMAC) 可以純化未融合His-tag的雞傳染性華氏囊病病毒 (Infectious Bursal Disease Virus, IBDV),以及其結構蛋白VP2所組裝的次病毒顆粒 (Subviral Particle, SVP),且經由結構推測可能是次病毒顆粒表面的胺基酸提供與鎳離子吸附的親和力。此外先前的研究亦指出IBDV VP2胺基酸位置206到350之間包含病毒的中和性抗原決定區,因此任一胺基酸變動都可能導致病毒的抗原變異,並有其他相關研究指出胺基酸253, 279, 284可調控病毒的致病力及進入細胞的機轉。
本實驗首先利用昆蟲細胞表現系統生產點突變VP2-441-H249.253A、 VP2-441-H253A及VP2-441-H249A 蛋白做為研究材料,經由蔗糖超高速離心純化後,藉由穿透式電子顯微鏡觀察次病毒顆粒。並以不同酸鹼值 (power of hydrogen, pH) 的緩衝溶液和不同濃度咪唑 (Imidazole) 進行沖提並藉由分子量篩選的高效能液相層析系統 (High Performance Liquid Chromatography, HPLC) 分析,經由上述實驗證實SVP表面的His253是和Ni-NTA吸附主要的胺基酸。之後進行等溫平衡吸附實驗得到每毫升Ni-NTA可以吸附的蛋白分子莫耳數 (Q),可得到VP2-441與Ni-NTA吸附達飽和之值 (Q) 為7.9 x 10-10 moles/ml Ni-NTA;H249A和H253A之Q值分別為7.3 x 10-10 and 3.5 x 10-10 moles/ml Ni-NTA,但H249.253A因為喪失了與鎳離子的吸附能力並無飽和值,所以取各組之Q值平均可得到2.6 x 10-11 moles/ml Ni-NTA。更進一步研究 H249 和 H253 是否會影響免疫原性,經由單株抗體 MAb SVP-1 和 MAb SVP-4 辨認 wild type VP2-441 SVP 以及突變 His249 與 His253 形成之 SVP,發現突變株均比 wild type VP2-441 SVP 與 mAb1 和 mAb4 結合效率要高,且經由雞隻免疫實驗結果發現VP2-441-H253A產生中和性抗體與VP2-specific IgG 力價均較 wild type VP2-441略為上升,因此推測 His253 可能位於影響IBDV免疫原性之位置。

In previous studies, we have confirmed that both of infectious bursal disease virus (IBDV) virions and the subviral particles (SVPs) composed of IBDV VP2 protein can be purified by immobilized metal ion affinity chromatography (IMAC). This suggests some amino acid residues on the surface of IBDV virions mediate the interaction with Ni2+ metal ions. Earlier reports indicate the region from residue 206 to 350 in VP2 protein contain the major immunogenic determinants. Therefore, any mutations on this region may result in IBDV antigenic variation. Furthermore, other resides include 253, 279, and 284 may determine the viral pathogenicity or play a key role on viral entry.
In this study, we focus on two histidine residues (His249 and His253) and investigate its importance on the interaction with Ni2+ ion and the effect on the immunogenicity. For these purposes, three VP2-441 variants, H249A, H253A, and H249.253A, were constructed and expressed by baculovirus expression system. The particle morphology of these variants examined by transmission electron microscopy (TEM) was similar to the authentic VP2-441 SVPs, exhibiting that the mutation from histidine to alanine doesn't affect the particle self-assembly. The binding strength of these variants with immobilized Ni2+ ion was measured by quantitating the unbound VP2 protein after a series of wash with various pH and imidazole concentrations. The results demonstrated that the His253 on the particle surface was the major amino acid contributes to the interaction with Ni2+ ion. Afterward, the adsorption of VP2-441 SVP to Ni2+-NTA was increased with the concentration of free VP2. The bound protein per volume of resin (Q) for VP2-441 SVP is 7.9 x 10-10 moles/ml Ni-NTA. The saturated Q of H249A and H253A reached to 7.3 x 10-10 and 3.5 x 10-10 moles/ml Ni-NTA, respectively. The equilibrium adsorption isoterm of H249.253A SVP to Ni-NTA had no accordance to the concentration of SVP and the average Q value was 2.6 x 10-11 moles/ml Ni-NTA, which is the lowest among the four SVPs.
For investigating the effect of such mutation on its immunogenicity, the corresponding SVPs were recognized by two neutralizing monoclonal antibodies (MAb SVP-1 and MAb SVP-4) against IBDV in an ELISA test. The data have shown such mutations offer the SVP a better binding affinity to the two neutralizing monoclonal antibodies. The protections afforded by three VP2-441 SVP variants were also compared by immunizing the specific-pathogen-free chickens and following with a homologous virus challenge. The sera collected from the chickens immunized with H253A SVPs have a higher virus neutralizing (VN) titer than authentic VP2-441 SVPs did. These suggested that the His253 may locate on the center of immunogenic determinants and mutation to alanine has fine-tuned the local structure to fit the binding pocket of neutralization antibody much more tightly.
其他識別: U0005-2508200819404500
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