Please use this identifier to cite or link to this item:
Calcium dependent protein kinase, OSCK1 plays critical roles in rice pollen germination
|關鍵字:||rice;水稻;CDPK;pollen;confocal microscope;結鈣激活酵素;花粉;共軛聚焦顯微鏡||出版社:||生物科技學研究所||引用:||李鐘財 (2000) 水稻花粉成熟期專一性表現基因OSCK1之選殖與分析. 國立中興大學, 台中市 汪承偉 (2003) 利用酵母菌雙雜交法篩選可與OSCK1結合的水稻花粉蛋白質. 國立中興大學, 台中市 王怡尹 (2005) 以轉基因植物分析水稻花粉結鈣激活酵素之功能. 國立中興大學, 台中市 林立菁 (2005) 以酵母菌雙雜交法系統分析水稻結鈣激活酵素與其結合蛋白質之交互作用. 國立中興大學, 台中市 林忠威 (2007) 水稻結鈣激活酵素基因群之表現分布及其僅於花粉大量表達成員之功能探討. 國立中興大學, 台中市 陳婉潔 (2003) 水稻花粉結鈣激活酵素OSCK1之基因表現、蛋白質胞內分部位置與基因轉殖植物分析. 國立中興大學, 台中市 童嬿融 (2004) 第一部份 : 水稻OSCK1基因靜默轉殖植物之分析、第二部份 : 水稻OIP基因表現模式之分析. 國立中興大學, 台中市 Anil VS, Harmon AC, Rao KS (2003) Temporal association of Ca(2+)-dependent protein kinase with oil bodies during seed development in Santalum album L.: its biochemical characterization and significance. Plant Cell Physiol 44: 367-376 Ariizumi T, Hatakeyama K, Hinata K, Inatsugi R, Nishida I, Sato S, Kato T, Tabata S, Toriyama K (2004) Disruption of the novel plant protein NEF1 affects lipid accumulation in the plastids of the tapetum and exine formation of pollen, resulting in male sterility in Arabidopsis thaliana. Plant J 39: 170-181 Asano T, Kunieda N, Omura Y, Ibe H, Kawasaki T, Takano M, Sato M, Furuhashi H, Mujin T, Takaiwa F, Wu Cy CY, Tada Y, Satozawa T, Sakamoto M, Shimada H (2002) Rice SPK, a calmodulin-like domain protein kinase, is required for storage product accumulation during seed development: phosphorylation of sucrose synthase is a possible factor. Plant Cell 14: 619-628 Asano T, Tanaka N, Yang G, Hayashi N, Komatsu S (2005) Genome-wide identification of the rice calcium-dependent protein kinase and its closely related kinase gene families: comprehensive analysis of the CDPKs gene family in rice. Plant Cell Physiol 46: 356-366 Bamburg JR (1999) Proteins of the ADF/cofilin family: essential regulators of actin dynamics. Annu Rev Cell Dev Biol 15: 185-230 Bibikova TN, Zhigilei A, Gilroy S (1997) Root hair growth in Arabidopsis thaliana is directed by calcium and an endogenous polarity. Planta 203: 495-505 Blancaflor EB (2002) The cytoskeleton and gravitropism in higher plants. J Plant Growth Regul 21: 120-136 Bosch M, Cheung AY, Hepler PK (2005) Pectin methylesterase, a regulator of pollen tube growth. Plant Physiol 138: 1334-1346 Chehab EW, Patharkar OR, Cushman JC (2007) Isolation and characterization of a novel v-SNARE family protein that interacts with a calcium-dependent protein kinase from the common ice plant, Mesembryanthemum crystallinum. Planta 225: 783-799 Chehab EW, Patharkar OR, Hegeman AD, Taybi T, Cushman JC (2004) Autophosphorylation and subcellular localization dynamics of a salt- and water deficit-induced calcium-dependent protein kinase from ice plant. Plant Physiol 135: 1430-1446 Chen CY-h, Cheung, A. Y., and Wu, H. -m. (2003) Rac-like GTPase and actin depolymerizing factor (ADF) mediate pollen germination and tube growth. Plant Cell 15: 237-249 Chen CY, Wong EI, Vidali L, Estavillo A, Hepler PK, Wu HM, Cheung AY (2002) The regulation of actin organization by actin-depolymerizing factor in elongating pollen tubes. Plant Cell 14: 2175-2190 Chen YC, McCormick S (1996) sidecar pollen, an Arabidopsis thaliana male gametophytic mutant with aberrant cell divisions during pollen development. Development 122: 3243-3253 Cheng SH, Willmann MR, Chen HC, Sheen J (2002) Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family. Plant Physiol 129: 469-485 Dammann C, Ichida A, Hong B, Romanowsky SM, Hrabak EM, Harmon AC, Pickard BG, Harper JF (2003) Subcellular targeting of nine calcium-dependent protein kinase isoforms from Arabidopsis. Plant Physiol 132: 1840-1848 Datta R, Chamusco KC, Chourey PS (2002) Starch biosynthesis during pollen maturation is associated with altered patterns of gene expression in maize. Plant Physiol 130: 1645-1656 Derksen J, Ruttens, T., van Amstel, T., de Win, A., Doris, F., and Steer, M. (1995) Regulation of polen tube growth. Acta Botany Neerl. 44: 93-119 Estruch JJ, Kadwell S, Merlin E, Crossland L (1994) Cloning and characterization of a maize pollen-specific calcium-dependent calmodulin-independent protein kinase. Proc Natl Acad Sci U S A 91: 8837-8841 Evans NH, McAinsh MR, Hetherington AM (2001) Calcium oscillations in higher plants. Curr Opin Plant Biol 4: 415-420 Geitmann A, Snowman BN, Emons AM, Franklin-Tong VE (2000) Alterations in the actin cytoskeleton of pollen tubes are induced by the self-incompatibility reaction in Papaver rhoeas. Plant Cell 12: 1239-1251 Gu Y, Fu Y, Dowd P, Li S, Vernoud V, Gilroy S, Yang Z (2005) A Rho family GTPase controls actin dynamics and tip growth via two counteracting downstream pathways in pollen tubes. J Cell Biol 169: 127-138 Han MJ, Jung KH, Yi G, Lee DY, An G (2006) Rice Immature Pollen 1 (RIP1) is a regulator of late pollen development. Plant Cell Physiol 47: 1457-1472 Harmon AC, Gribskov M, Harper JF (2000) CDPKs - a kinase for every Ca2+ signal? Trends Plant Sci 5: 154-159 Harper JF, Breton G, Harmon A (2004) Decoding Ca(2+) signals through plant protein kinases. Annu Rev Plant Biol 55: 263-288 Harper JF, Sussman MR, Schaller GE, Putnam-Evans C, Charbonneau H, Harmon AC (1991) A calcium-dependent protein kinase with a regulatory domain similar to calmodulin. Science 252: 951-954 Hepler PK (1997) Tip growth in pollen tubes: Calcium leads the way. Trends Plant Sci 2: 79-80 Hepler PK, Vidali L, Cheung AY (2001) Polarized cell growth in higher plants. Annu Rev Cell Dev Biol 17: 159-187 Higgs HN, Pollard TD (2001) Regulation of actin filament network formation through ARP2/3 complex: activation by a diverse array of proteins. Annu Rev Biochem 70: 649-676 Hrabak EM, Chan CW, Gribskov M, Harper JF, Choi JH, Halford N, Kudla J, Luan S, Nimmo HG, Sussman MR, Thomas M, Walker-Simmons K, Zhu JK, Harmon AC (2003) The Arabidopsis CDPK-SnRK superfamily of protein kinases. Plant Physiol 132: 666-680 Huang S, Blanchoin L, Chaudhry F, Franklin-Tong VE, Staiger CJ (2004) A gelsolin-like protein from Papaver rhoeas pollen (PrABP80) stimulates calcium-regulated severing and depolymerization of actin filaments. J Biol Chem 279: 23364-23375 Iwakawa H, Shinmyo A, Sekine M (2006) Arabidopsis CDKA;1, a cdc2 homologue, controls proliferation of generative cells in male gametogenesis. Plant J 45: 819-831 Iwata Y, Kuriyama M, Nakakita M, Kojima H, Ohto M, Nakamura K (1998) Characterization of a calcium-dependent protein kinase of tobacco leaves that is associated with the plasma membrane and is inducible by sucrose. Plant Cell Physiol 39: 1176-1183 Kawasaki T, Hayashida N, Baba T, Shinozaki K, Shimada H (1993) The gene encoding a calcium-dependent protein kinase located near the sbe1 gene encoding starch branching enzyme I is specifically expressed in developing rice seeds. Gene 129: 183-189 Knight H, Knight MR (2001) Abiotic stress signalling pathways: specificity and cross-talk. Trends Plant Sci 6: 262-267 Kost B, Lemichez E, Spielhofer P, Hong Y, Tolias K, Carpenter C, Chua NH (1999) Rac homologues and compartmentalized phosphatidylinositol 4, 5-bisphosphate act in a common pathway to regulate polar pollen tube growth. J Cell Biol 145: 317-330 Lalanne E, Twell D (2002) Genetic control of male germ unit organization in Arabidopsis. Plant Physiol 129: 865-875 Lee SS, Cho HS, Yoon GM, Ahn JW, Kim HH, Pai HS (2003) Interaction of NtCDPK1 calcium-dependent protein kinase with NtRpn3 regulatory subunit of the 26S proteasome in Nicotiana tabacum. Plant J 33: 825-840 Li H, Lin Y, Heath RM, Zhu MX, Yang Z (1999) Control of pollen tube tip growth by a Rop GTPase-dependent pathway that leads to tip-localized calcium influx. Plant Cell 11: 1731-1742 Li YQ, Zhang, H. Q., Pierso, E. S., Huang, H. F., Hepler, P. K., and Cresti, M. (1996) Enforced growth-rate fluctuatuion causes pectin ring formation in the cell wall of Lilium longiflorum pollen tubes. Planta 200: 41-49 Lin Y, Yang Z (1997) Inhibition of Pollen Tube Elongation by Microinjected Anti-Rop1Ps Antibodies Suggests a Crucial Role for Rho-Type GTPases in the Control of Tip Growth. Plant Cell 9: 1647-1659 Lovy-Wheeler A, Wilsen KL, Baskin TI, Hepler PK (2005) Enhanced fixation reveals the apical cortical fringe of actin filaments as a consistent feature of the pollen tube. Planta 221: 95-104 Lu SX, Hrabak EM (2002) An Arabidopsis calcium-dependent protein kinase is associated with the endoplasmic reticulum. Plant Physiol 128: 1008-1021 Malho R, Read ND, Trewavas AJ, Pais MS (1995) Calcium Channel Activity during Pollen Tube Growth and Reorientation. Plant Cell 7: 1173-1184 Malho R, Read, N. D., Pais, M, and Trewavas, A. J. (1994) Role of cytosolic calcium in the reorientation of pollen tube growth. plant Journal 5: 331-341 Malho R, Trewavas AJ (1996) Localized Apical Increases of Cytosolic Free Calcium Control Pollen Tube Orientation. Plant Cell 8: 1935-1949 McCormick S (1993) Male Gametophyte Development. Plant Cell 5: 1265-1275 McCormick S (2004) Control of male gametophyte development. Plant Cell 16 Suppl: S142-153 McCubbin AG, Ritchie SM, Swanson SJ, Gilroy S (2004) The calcium-dependent protein kinase HvCDPK1 mediates the gibberellic acid response of the barley aleurone through regulation of vacuolar function. Plant J 39: 206-218 McGough A (1998) F-actin-binding proteins. Curr Opin Struct Biol 8: 166-176 Messerli M, Robinson KR (1997) Tip localized Ca2+ pulses are coincident with peak pulsatile growth rates in pollen tubes of Lilium longiflorum. J Cell Sci 110 ( Pt 11): 1269-1278 Moutinho A, Trewavas AJ, Malho R (1998) Relocation of a Ca2+-dependent protein kinase activity during pollen tube reorientation. Plant Cell 10: 1499-1510 Niewiadomski P, Knappe S, Geimer S, Fischer K, Schulz B, Unte US, Rosso MG, Ache P, Flugge UI, Schneider A (2005) The Arabidopsis plastidic glucose 6-phosphate/phosphate translocator GPT1 is essential for pollen maturation and embryo sac development. Plant Cell 17: 760-775 Nonomura K, Morohoshi A, Nakano M, Eiguchi M, Miyao A, Hirochika H, Kurata N (2007) A germ cell specific gene of the ARGONAUTE family is essential for the progression of premeiotic mitosis and meiosis during sporogenesis in rice. Plant Cell 19: 2583-2594 Obermeyer G, Weisenseel MH (1991) Calcium channel blocker and calmodulin antagonists affect the gradient of free calcium ions in lily pollen tubes. Eur J Cell Biol 56: 319-327 Patharkar OR, Cushman JC (2000) A stress-induced calcium-dependent protein kinase from Mesembryanthemum crystallinum phosphorylates a two-component pseudo-response regulator. Plant J 24: 679-691 Patharkar OR, Cushman JC (2006) A novel coiled-coil protein co-localizes and interacts with a calcium-dependent protein kinase in the common ice plant during low-humidity stress. Planta 225: 57-73 Pierson ES, Miller DD, Callaham DA, Shipley AM, Rivers BA, Cresti M, Hepler PK (1994) Pollen tube growth is coupled to the extracellular calcium ion flux and the intracellular calcium gradient: effect of BAPTA-type buffers and hypertonic media. Plant Cell 6: 1815-1828 Putnam-Evans C, Harmon, A. C., Palevitz, B. A., Fechheimer, M., and Cormier, M. J. (1989) Calcium-dependent protein kinase is localized with F-actin in plant cells. Cell Motility and the Cytoskeleton 12: 12-22 Raghavan V (1988) Anther and pollen development in rice (Oryza sativa). American Journal of Botany 75: 183-186 Rathore KS, Cork RJ, Robinson KR (1991) A cytoplasmic gradient of Ca2+ is correlated with the growth of lily pollen tubes. Dev Biol 148: 612-619 Roberts DMaH, A. C. (1992) Calcium-modulated proteins: targets of intracellular calcium signals in higher plants. Annual Review Plant Physiology and Plant Molecular Biology 43: 375-414 Saijo Y, Hata S, Kyozuka J, Shimamoto K, Izui K (2000) Over-expression of a single Ca2+-dependent protein kinase confers both cold and salt/drought tolerance on rice plants. Plant J 23: 319-327 Sanders D, Brownlee C, Harper JF (1999) Communicating with calcium. Plant Cell 11: 691-706 Schaller GE, Harmon AC, Sussman MR (1992) Characterization of a calcium- and lipid-dependent protein kinase associated with the plasma membrane of oat. Biochemistry 31: 1721-1727 Staiger CJ, Gibbon, B. C., Kovar, D. R., and Zonlia, L. E. (1997) Profilin and actin depolymerizing factor: Modulators of actin organization in plants. Trends Plant Sci 2: 275-281 Steer MW, and Steer, J. M. (1989) Pollen tube tip growth. New Phytol. 111: 323-358 Tao J, Zhang L, Chong K, Wang T (2007) OsRAD21-3, an orthologue of yeast RAD21, is required for pollen development in Oryza sativa. Plant J 51: 919-930 Toullec D, Pianetti P, Coste H, Bellevergue P, Grand-Perret T, Ajakane M, Baudet V, Boissin P, Boursier E, Loriolle F, et al. (1991) The bisindolylmaleimide GF 109203X is a potent and selective inhibitor of protein kinase C. J Biol Chem 266: 15771-15781 Twell D, Park, S. K. and Lalanne, E. (1998) Asymetric division and cell-fate determination in developing pollen. Trends Plant Sci 3: 305-310 Verhey SD, Gaiser JC, Lomax TL (1993) Protein Kinases in Zucchini (Characterization of Calcium-Requiring Plasma Membrane Kinases). Plant Physiol 103: 413-419 Vidali L, McKenna ST, Hepler PK (2001) Actin polymerization is essential for pollen tube growth. Mol Biol Cell 12: 2534-2545 Vitart VV, Christodoulou J, Huang JF, Chazin WJ, Harper JF (2000) Intramolecular activation of a Ca(2+)-dependent protein kinase is disrupted by insertions in the tether that connects the calmodulin-like domain to the kinase. Biochemistry 39: 12102 Wan B, Lin Y, Mou T (2007) Expression of rice Ca(2+)-dependent protein kinases (CDPKs) genes under different environmental stresses. FEBS Lett 581: 1179-1189 Woo MO, Ham TH, Ji HS, Choi MS, Jiang W, Chu SH, Piao R, Chin JH, Kim JA, Park BS, Seo HS, Jwa NS, McCouch S, Koh HJ (2008) Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.). Plant J 54: 190-204 Yoon GM, Cho HS, Ha HJ, Liu JR, Lee HS (1999) Characterization of NtCDPK1, a calcium-dependent protein kinase gene in Nicotiana tabacum, and the activity of its encoded protein. Plant Mol Biol 39: 991-1001 Yoon GM, Dowd PE, Gilroy S, McCubbin AG (2006) Calcium-dependent protein kinase isoforms in Petunia have distinct functions in pollen tube growth, including regulating polarity. Plant Cell 18: 867-878 Zheng ZL, Yang Z (2000) The Rop GTPase: an emerging signaling switch in plants. Plant Mol Biol 44: 1-9 Zuo L, Li S, Chu M, Wang S, Deng Q, Ding L, Zhang J, Wen Y, Zheng A, Li P (2008) Phenotypic characterization, genetic analysis, and molecular mapping of a new mutant gene for male sterility in rice. Genome 51: 303-308||摘要:||
高等植物的成熟花粉在乾燥環境下自花藥釋出，必須落在可以相容的雌蕊柱頭上，花粉開始萌發，花粉管突出並伸長穿入柱頭，兩個精細胞核因此可分別與卵細胞及極核結合，完成授粉。已知鈣離子是調節花粉管生長的重要因子之ㄧ，卻很少知道在此過程中鈣離子的下游受動器為何，或鈣離子又是如何調節花粉管生長的機制？由於結鈣激活酵素（calcium dependent protein kinases，CDPKs）會受到鈣離子調控，故成為可能的候選基因。本實驗室過去曾在花粉成熟時期篩選到一個CDPK基因，命名為OSCK1，推測其功能與調控花粉發育、花粉管萌發或伸長相關，欲利用轉殖植物以了解OSCK1在花粉扮演的角色。實驗設計利用具有花粉專一性的Zm13啟動子，分別驅動OSCK1或其他三種突變型基因於水稻中大量表現，三種突變型基因分別可產生無法附著於細胞膜的G2A蛋白質、失去激活酵素活性的CI蛋白質、及不受鈣離子控制並且永遠具有酵素活性的CA蛋白質，與載體p35ST轉殖株相比較，發現各轉殖株的營養生長與植株外觀均無明顯差異。以掃描式電子顯微鏡觀察各轉殖品系之花粉形態，發現野生型與G2A轉植株花粉形態多正常，但CI與CA轉植株則多出現皺縮之花粉。檢查受粉後柱頭上的花粉萌發情形發現，CI轉殖株柱頭上雖佈滿花粉，卻多不萌發，且它的in vitro花粉萌發率與稔實率亦顯著低於p35ST轉殖株。因此提出OSCK1的功能可能影響花粉發育與後續之花粉管萌發。
Pollen is released from the anther in a dehydrated state. Upon contact with a compatible stigma, pollen will germinate and pollen tube elongate through the style in order to have two sperm nuclei fused with the egg cell and the central cell, respectively, known as the double fertilization. Calcium is a key regulator of pollen tube growth, but little is known concerning the identity of downstream effectors of Ca2+ in this process or precisely how Ca2+ dynamics modulate the tip growth machinery. One class of likely candidates for such modulators is the calcium-dependent protein kinases (CDPKs). As OSCK1 (Oryza sativa CDPK 1) gene was previously identified to be expressed predominantly in the mature pollen of rice, it may involve in control of pollen development or tube elongation. Transgenic plant analysis was employed to investigate roles of OSCK1 in this study. Wild-type or mutated OSCK1 genes including G2A (non-membrane form), CI (catalytically inactive), or CA (constitutively active), driven by the maize pollen-specific promoter Zm13, were constructed and transformed into rice. No specific phenotypes were observed at the vegetative stage for all transgenic plants. Pollen morphology examined by scanning electron microscope was found to be basically normal for OSCK1 and G2A transgenic lines. However, severly shrunken pollen was found in various CI and CA lines. Interestingly, examination of stigma after pollination revealed that, among abundant pollen remained on stigma, almost none germinate in one CI transgenic line. Moreover, its in vitro pollen germination rate and fertility rate was significantly lower than that of control. These data indicate that OSCK1 may affect pollen development and consequencly, pollen germination processes.
To further address how OSCK1 affect the pollen germination process, plasmids which can overproduce GFP-fused OSCK1 proteins were bombarded into the germinating lily pollen. The bombardments were carried out either alone or together with plasmid that encodes OIP30, which is a putative downstream substrate for OSCK1 previously identified by a yeast two-hybrid screen. Except for the OSCK1-G2A protein which remained in cytosol, wild-type and all other mutated OSCK1 proteins were mainly localized on the plasma membrane when bombarded alone, demonstrated that OSCK1 was bound to membrane via myristoylation. As predicted according to the sequence information, most of the OIP30 signals were in cytosol when expressed alone. However, its distribution was changed to be partially membrane-bound when co-bombarded with OSCK1, suggesting their protein-protein interactions in vivo. Since OIP30 is likely a helicase, it is expected to execute its enzyme activity in the nucleus. We therefore use DAPI to trace nucleus in the bombared pollen. Among various samples examined, OIP30-EYFP, when co-bombared with OSCK1-G2A or OSCK1-CA, were found to be partially located within nucleus. We conclude that OIP30 may be a downstream substrate for OSCK1 in pollen. Overexpression of the catalytically inactive OSCK1-CI protein in the rice pollen may interfere the phosphorylation or nucleus-import of OIP30 by OSCK1, therefore impede the pollen germination process.
|Appears in Collections:||生物科技學研究所|
Show full item record
TAIR Related Article
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.