Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/36221
標題: 以自營性螢光油體偵測腫瘤細胞
Detection of tumor cells by self-fluorescent oil bodies
作者: 王信富
Wang, Hesin-Fu
關鍵字: oil body;油體;tumor cells;腫瘤細胞
出版社: 生物科技學研究所
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摘要: 
植物種子儲存三酸甘油酯(triacylglycerol) 做為發芽所需之量。三酸甘油酯儲存於一種植物種子特有的胞器稱為油體。目前所提出的油體構造模型是一團三酸甘油酯包在一層磷脂質(phospholipid)內,此磷脂質層鑲滿了構造蛋白質叫油體膜蛋白(oleosin)及一些微量的蛋白質。
在本研究中,我們將oleosin與一個具有特殊功用的胜肽融合,以大腸桿菌大量生產此融合蛋白質,接著運用油體重組技術製作人造油體。經由不同pH值、油種及油種用量等條件檢測,得知以芝麻油建構之人造油體在pH 7.5時之穩定度最佳、且顆粒大小一致。最後,我們將所建構的人造油體與腫瘤細胞株作用,結果顯示此人造油體可以專一的與腫瘤細胞結合。

Plant seeds store triacylglycerols (TAGs) as energy sources for germination and postgerminative growth of seedlings. The storage TAGs are confined to discrete spherical organelles called oil bodies. An oil body contains a TAG matrix surrounded by a monolayer of phospholipids (PLs) embedded with abundant oleosins and some minor proteins of higher molecular mass.
In this study, oleosin was fused with a peptide with a special function. The fused protein was overproduced n in Escherichia coli and then utilized for reconstitution of artificial oil bodies (AOBs). After a systematic investigation, AOBs made of sesame oil at pH 7.5 were found to exhibit the highest stability with a size distribution of homology. Finally, after co-incubation of AOBs with several lines of tumor cells, the result shows that the AOBs elicit the ability to bind to tumor cells in a very specific manner.
URI: http://hdl.handle.net/11455/36221
其他識別: U0005-2307200916420400
Appears in Collections:生物科技學研究所

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