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dc.contributor.authorTseng, Ching-Hanen_US
dc.identifier.citationAhlquist, P., Noueiry, A. O., Lee, W. M., Kushner, D. B., and Dye, B. T. (2003). Host factors in positive-strand RNA virus genome replication. J Virol 77(15), 8181-6. Al-Kaff, N. S., Covey, S. N., Kreike, M. M., Page, A. M., Pinder, R., and Dale, P. J. (1998). Transcriptional and posttranscriptional plant gene silencing in response to a pathogen. Science 279(5359), 2113-5. Bachem, C. W., van der Hoeven, R. S., de Bruijn, S. M., Vreugdenhil, D., Zabeau, M., and Visser, R. G. (1996). Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: analysis of gene expression during potato tuber development. Plant J 9(5), 745-53. Bar, M., and Avni, A. (2009). EHD2 inhibits ligand-induced endocytosis and signaling of the leucine-rich repeat receptor-like protein LeEix2. Plant J 59(4), 600-11. Beauchemin, C., Boutet, N., and Laliberte, J. F. (2007). Visualization of the interaction between the precursors of VPg, the viral protein linked to the genome of turnip mosaic virus, and the translation eukaryotic initiation factor iso 4E in Planta. J Virol 81(2), 775-82. Beauchemin, C., and Laliberte, J. F. (2007). The poly(A) binding protein is internalized in virus-induced vesicles or redistributed to the nucleolus during turnip mosaic virus infection. J Virol 81(20), 10905-13. Beck, D. L., Guilford, P. J., Voot, D. M., Andersen, M. T., and Forster, R. L. (1991). Triple gene block proteins of white clover mosaic potexvirus are required for transport. Virology 183(2), 695-702. Bella, J., Hindle, K. L., McEwan, P. A., and Lovell, S. C. (2008). The leucine-rich repeat structure. Cell Mol Life Sci 65(15), 2307-33. Bendahmane, A., Kanyuka, K., and Baulcombe, D. C. (1999). The Rx gene from potato controls separate virus resistance and cell death responses. Plant Cell 11(5), 781-92. Chautard, E., Thierry-Mieg, N., and Ricard-Blum, S. (2009). Interaction networks: from protein functions to drug discovery. A review. Pathol Biol (Paris) 57(4), 324-33. Chen, M. H., and Citovsky, V. (2003). Systemic movement of a tobamovirus requires host cell pectin methylesterase. Plant J 35(3), 386-92. Cheng, S. F., Huang, Y. P., Wu, Z. R., Hu, C. C., Hsu, Y. H., and Tsai, C. H. (2010). Identification of differentially expressed genes induced by Bamboo mosaic virus infection in Nicotiana benthamiana by cDNA-amplified fragment length polymorphism. BMC Plant Biol 10(1), 286. Chicas, A., and Macino, G. (2001). Characteristics of post-transcriptional gene silencing. EMBO Rep 2(11), 992-6. Cotton, S., Grangeon, R., Thivierge, K., Mathieu, I., Ide, C., Wei, T., Wang, A., and Laliberte, J. F. (2009). Turnip mosaic virus RNA replication complex vesicles are mobile, align with microfilaments, and are each derived from a single viral genome. J Virol 83(20), 10460-71. Covey, S. N., AlKaff, N. S., Langara, A., and Turner, D. S. (1997). Plants combat infection by gene silencing. Nature 385(6619), 781-782. Dangl, J. L., and Jones, J. D. (2001). Plant pathogens and integrated defence responses to infection. Nature 411(6839), 826-33. Ditt, R. F., Nester, E. W., and Comai, L. (2001). Plant gene expression response to Agrobacterium tumefaciens. Proc Natl Acad Sci U S A 98(19), 10954-9. Dixon, M. S., Golstein, C., Thomas, C. M., van Der Biezen, E. A., and Jones, J. D. (2000). Genetic complexity of pathogen perception by plants: the example of Rcr3, a tomato gene required specifically by Cf-2. Proc Natl Acad Sci U S A 97(16), 8807-14. Dixon, M. S., Hatzixanthis, K., Jones, D. A., Harrison, K., and Jones, J. D. (1998). The tomato Cf-5 disease resistance gene and six homologs show pronounced allelic variation in leucine-rich repeat copy number. Plant Cell 10(11), 1915-25. Dixon, M. S., Jones, D. A., Keddie, J. S., Thomas, C. M., Harrison, K., and Jones, J. D. (1996). The tomato Cf-2 disease resistance locus comprises two functional genes encoding leucine-rich repeat proteins. Cell 84(3), 451-9. Dufresne, P. J., Thivierge, K., Cotton, S., Beauchemin, C., Ide, C., Ubalijoro, E., Laliberte, J. F., and Fortin, M. G. (2008). Heat shock 70 protein interaction with Turnip mosaic virus RNA-dependent RNA polymerase within virus-induced membrane vesicles. Virology 374(1), 217-27. Durrant, W. E., Rowland, O., Piedras, P., Hammond-Kosack, K. E., and Jones, J. D. (2000). cDNA-AFLP reveals a striking overlap in race-specific resistance and wound response gene expression profiles. Plant Cell 12(6), 963-77. Hammond-Kosack, K. E., Silverman, P., Raskin, I., and Jones, J. (1996). Race-Specific Elicitors of Cladosporium fulvum Induce Changes in Cell Morphology and the Synthesis of Ethylene and Salicylic Acid in Tomato Plants Carrying the Corresponding Cf Disease Resistance Gene. Plant Physiol 110(4), 1381-1394. Huang, C. Y., and Tsai, C. H. (1998). Evolution of bamboo mosaic virus in a nonsystemic host results in mutations in the helicase-like domain that cause reduced RNA accumulation. Virus Res 58(1-2), 127-36. Huang, Y. L., Han, Y. T., Chang, Y. T., Hsu, Y. H., and Meng, M. (2004). Critical residues for GTP methylation and formation of the covalent m7GMP-enzyme intermediate in the capping enzyme domain of bamboo mosaic virus. J Virol 78(3), 1271-80. Jones, D. A., and Jones, J. D. G. (1996). The roles of leucine rich repeats in plant defences. Adv. Bot. Res. Adv. Plant Pathol. 24(90-167). Kajava, A. V. (1998). Structural diversity of leucine-rich repeat proteins. Mol. Biol. 277, 519-527. Kjemtrup, S., Sampson, K. S., Peele, C. G., Nguyen, L. V., Conkling, M. A., Thompson, W. F., and Robertson, D. (1998). Gene silencing from plant DNA carried by a Geminivirus. Plant J 14(1), 91-100. Kobe, B., and Deisenhofer, J. (1994). The leucine-rich repeat: a versatile binding motif. Trends Biochem Sci 19(10), 415-21. Kobe B Fau - Kajava, A. V., and Kajava, A. V. (2001). The leucine-rich repeat as a protein recognition motif(0959-440X (Print)). Kumagai, M. H., Donson, J., della-Cioppa, G., Harvey, D., Hanley, K., and Grill, L. K. (1995). Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA. Proc Natl Acad Sci U S A 92(5), 1679-83. Li, Y. I., Chen, Y. J., Hsu, Y. H., and Meng, M. (2001a). Characterization of the AdoMet-dependent guanylyltransferase activity that is associated with the N terminus of bamboo mosaic virus replicase. J Virol 75(2), 782-8. Li, Y. I., Cheng, Y. M., Huang, Y. L., Tsai, C. H., Hsu, Y. H., and Meng, M. (1998). Identification and characterization of the Escherichia coli-expressed RNA-dependent RNA polymerase of bamboo mosaic virus. J Virol 72(12), 10093-9. Li, Y. I., Shih, T. W., Hsu, Y. H., Han, Y. T., Huang, Y. L., and Meng, M. (2001b). The helicase-like domain of plant potexvirus replicase participates in formation of RNA 5'' cap structure by exhibiting RNA 5''-triphosphatase activity. J Virol 75(24), 12114-20. Lin, M. K., Chang, B. Y., Liao, J. T., Lin, N. S., and Hsu, Y. H. (2004). Arg-16 and Arg-21 in the N-terminal region of the triple-gene-block protein 1 of Bamboo mosaic virus are essential for virus movement. J Gen Virol 85(Pt 1), 251-9. Lin, M. K., Hu, C. C., Lin, N. S., Chang, B. Y., and Hsu, Y. H. (2006). Movement of potexviruses requires species-specific interactions among the cognate triple gene block proteins, as revealed by a trans-complementation assay based on the bamboo mosaic virus satellite RNA-mediated expression system. J Gen Virol 87(Pt 5), 1357-67. Lin, M. T., Kitajima, E. W., Cupertino, F. P., and Costa, C. L. (1977). Partial Purification and Some Properties of Bamboo Mosaic Virus. Phytopathology 67, 1439-43. Lin, N. S., Chai, Y. J., Huang, T. Y., Chow, T. Y., and Hsu, Y. H. (1993). Incidence of Bamboo Mosaic Potexvirus in Taiwan. Plant Disease 77(5), 448-50. Lin, N. S., and Hsu, Y. H. (1994). A satellite RNA associated with bamboo mosaic potexvirus. Virology 202(2), 707-14. Lin, N. S., Lin, B. Y., Lo, N. W., Hu, C. C., Chow, T. Y., and Hsu, Y. H. (1994). Nucleotide sequence of the genomic RNA of bamboo mosaic potexvirus. J Gen Virol 75 ( Pt 9), 2513-8. Liu, Y., Jiang, Y., Qiao, D. R., and Cao, Y. (2002). [The mechanism and application of posttranscriptional gene silencing]. Sheng Wu Gong Cheng Xue Bao 18(2), 140-3. Liu, Y., Schiff, M., and Dinesh-Kumar, S. P. (2002). Virus-induced gene silencing in tomato. Plant J 31(6), 777-86. Lough, T. J., Netzler, N. E., Emerson, S. J., Sutherland, P., Carr, F., Beck, D. L., Lucas, W. J., and Forster, R. L. (2000). Cell-to-cell movement of potexviruses: evidence for a ribonucleoprotein complex involving the coat protein and first triple gene block protein. Mol Plant Microbe Interact 13(9), 962-74. Lough, T. J., Shash, K., Xoconostle-Cázares, B., Hofstra, K. R., Beck, D. L., Balmori, E., Forster, R. L. S., and Lucas, W. J. (1998). Molecular Dissection of the Mechanism by Which Potexvirus Triple Gene Block Proteins Mediate Cell-to-Cell Transport of Infectious RNA. Mol Plant Microbe interact 11(8), 801-814. Lucas, W. J. (2006). Plant viral movement proteins: agents for cell-to-cell trafficking of viral genomes. Virology 344(1), 169-84. Matsushima, N., Miyashita, H., Mikami, T., and Kuroki, Y. (2010). A nested leucine rich repeat (LRR) domain: the precursor of LRRs is a ten or eleven residue motif. BMC Microbiol 10, 235. Moffett, P. (2009). Mechanisms of recognition in dominant R gene mediated resistance. Adv Virus Res 75, 1-33. Money, T., Reader, S., Qu, L. J., Dunford, R. P., and Moore, G. (1996). AFLP-based mRNA fingerprinting. Nucleic Acids Res 24(13), 2616-7. Petty, I. T., French, R., Jones, R. W., and Jackson, A. O. (1990). Identification of barley stripe mosaic virus genes involved in viral RNA replication and systemic movement. EMBO J 9(11), 3453-7. Qin, L., Overmars, H., Helder, J., Popeijus, H., van der Voort, J. R., Groenink, W., van Koert, P., Schots, A., Bakker, J., and Smant, G. (2000). An efficient cDNA-AFLP-based strategy for the identification of putative pathogenicity factors from the potato cyst nematode Globodera rostochiensis. Mol Plant Microbe Interact 13(8), 830-6. Ratcliff, F., Martin-Hernandez, A. M., and Baulcombe, D. C. (2001). Technical Advance. Tobacco rattle virus as a vector for analysis of gene function by silencing. Plant J 25(2), 237-45. Ratcliff, F. G., MacFarlane, S. A., and Baulcombe, D. C. (1999). Gene silencing without DNA. rna-mediated cross-protection between viruses. . Plant Cell 11(7), 1207-16. Rooney, H. C., Van''t Klooster, J. W., van der Hoorn, R. A., Joosten, M. H., Jones, J. D., and de Wit, P. J. (2005). Cladosporium Avr2 inhibits tomato Rcr3 protease required for Cf-2-dependent disease resistance. Science 308(5729), 1783-6. Ruiz, M. T., Voinnet, O., and Baulcombe, D. C. (1998). Initiation and maintenance of virus-induced gene silencing. Plant Cell 10(6), 937-46. Schepetilnikov, M. V., Manske, U., Solovyev, A. G., Zamyatnin, A. A. J., Schiemann, J., and Morozov, S. Y. (2005). The hydrophobic segment of Potato virus X TGBp3 is a major determinant of the protein intracellular trafficking. J Gen Virol 86(Pt 8), 2379-91. Seear, P. J., and Dixon, M. S. (2003). Variable leucine-rich repeats of tomato disease resistance genes Cf-2 and Cf-5 determine specificity. Mol Plant Pathol 4(3), 199-202. Solovyev, A. G., Savenkov, E. I., Agranovsky, A. A., and Morozov, S. Y. (1996). Comparisons of the genomic cis-elements and coding regions in RNA beta components of the hordeiviruses barley stripe mosaic virus, lychnis ringspot virus, and poa semilatent virus. Virology 219(1), 9-18. Takemoto, D., Hayashi, M., Doke, N., Mishimura, M., and Kawakita, K. (2000). Isolation of the gene for EILP, an elicitor-inducible LRR receptor-like protein, from tobacco by differential display. Plant Cell Physiol 41(4), 458-64. Tsai, C. H., and Dreher, T. W. (1991). Turnip yellow mosaic virus RNAs with anticodon loop substitutions that result in decreased valylation fail to replicate efficiently. J Virol 65(6), 3060-7. Uetz, P. (2002). Two-hybrid arrays. Curr Opin Chem Biol 6(1), 57-62. van der Biezen, E. A., Juwana, H., Parker, J. E., and Jones, J. D. (2000). cDNA-AFLP display for the isolation of Peronospora parasitica genes expressed during infection in Arabidopsis thaliana. Mol Plant Microbe Interact 13(8), 895-8. Verchot-Lubicz, J. (2005). A new cell-to-cell transport model for Potexviruses. Mol Plant Microbe Interact 18(4), 283-90. Yang, Y., Ding, B., and Baulcombe, D. C. (2000). Cell-to-cell movement of the 25K protein of potato virus X is regulated by three other viral proteins. Mol Plant Microbe Interact 13(6), 599-605. Zamyatnin, A. A., Jr., Solovyev, A. G., Sablina, A. A., Agranovsky, A. A., Katul, L., Vetten, H. J., Schiemann, J., Hinkkanen, A. E., Lehto, K., and Morozov, S. Y. (2002). Dual-colour imaging of membrane protein targeting directed by poa semilatent virus movement protein TGBp3 in plant and mammalian cells. J Gen Virol 83(Pt 3), 651-62en_US
dc.description.abstract竹嵌紋病毒(Bamboo mosaic virus, BaMV)是一種隸屬於Flexiviridae科Potexvirus屬的正股RNA病毒,是感染竹子的主要病毒之一,由於病毒的基因體較小,能轉譯出的蛋白有限,無法靠自己完成生活史,其需要仰賴宿主細胞提供蛋白質,協助完成複製、摺疊、移動等生命週期。因此,了解病毒及宿主基因間的互動,有助於我們發展對抗病毒的方法。而本實驗的研究目的,是尋找與病毒有關的宿主蛋白質,藉此了解病毒的繁殖機制。根據前人研究之 cDNA-amplified fragment length polymorphism (cDNA-AFLP)結果,我們挑選了一個宿主基因,其在菸草感染病毒後,表現量會明顯隨著時間增加,將之代稱為ACCT8-1,並取得此基因的片段序列。首先,為了比對出這個基因在宿主內可能的用途,我們使用Rapid Amplification of cDNA End (RACE)的技術,延長這個宿主基因的上下游片段,得到3199nt的序列,經過與National Center for Biotechnology Information (NCBI)資料庫比對後,發現ACCT8-1與Nicotiana tabacum的EILP (elicitor inducible LRR protein) gene 及Cladosporium fulvum抗病基因(Cladosporium resistance gene; cf-2 and cf-5)之序列相似。本研究便是以此基因為研究對象,以菸草脆裂病毒(Tobacco rattle virus)誘導基因靜默(virus induced gene silencing; VIGS) 的方法降低其基因的表現,進而以西方墨點法(western blot)來偵測這個基因對於BaMV在宿主內繁殖的影響,結果發現降低ACCT8-1基因表現量後,病毒鞘蛋白(coat protein)的累積量下降了40% (5dpi),而再以北方墨點法(northern blot)偵測病毒RNA累積量的實驗中,也得到相似的結果,但在馬鈴薯X病毒Potato virus X (PVX)及胡瓜嵌紋病毒Cucumber mosaic virus (CMV)中,並沒有發現病毒鞘蛋白累積量有顯著的差別。由此可發現,靜默ACCT8-1基因後,唯有竹嵌紋病毒的累積量有明顯的減少,再進一步再用西方墨點法(western blot)來偵測原生質體(protoplast)及接種葉其上第三片系統葉的病毒鞘蛋白(coat protein)的累積量,研究病毒繁殖量變少的原因,若原生質體(protoplast)中,病毒的累積量減少,則可能是由於病毒的複製量變少,但若是系統葉中的病毒鞘蛋白累積量下降,則可推測是病毒的移動受到了抑制。結果發現,靜默ACCT8-1基因後,系統葉上的病毒鞘蛋白累積量下降40%,但在原生質體中的累積量則只下降10%,由結果得知抑制此基因後,只會導致系統葉中病毒鞘蛋白的累積量明顯下降,因此推斷ACCT8-1基因可能主要是影響病毒的移動能力,因而在抑制其基因表現時,也同時影響了病毒的移動。而且在竹嵌紋病毒感染後觀察病斑的實驗中,也發現基因靜默的植物葉片病斑較小,由此更進一步支持以上的推論。zh_TW
dc.description.abstractBamboo mosaic virus (BaMV), belongs to genus Potexvirus of the family Flexiviridae, is a positive-sense RNA virus. Since the virus can not complete its infection cycle by itself, it needs host factors in different stages, such as RNA replication, folding, and spreading. Therefore, studying the interaction between virus and its host is important and useful in developing antivirus strategy. The aim of this study is identifying the host proteins that involve in the infection of BaMV. According to the previous study, many of differentially expressed genes from BaMV infected Nicotiana benthamiana plants were identified using cDNA-amplified fragment length polymorphism (cDNA-AFLP) technique. Then I focus on one of the upregulated cDNA fragments, ACCT8-1. Rapid amplification of cDNA ends (RACE) technique is used to extend the 5' and 3'-end of the cDNA fragments to 3199 nucleotides in length. The sequence the hypothetic protein derived from the coding region is blasted with the database in National Center for Biotechnology Information. ACCT8-1 is an ortholog of N. tabacum EILP (elicitor inducible LRR protein) gene and Cladosporium fulvum resistance gene (cf-2 and cf-5). The expression level of ACCT8-1 is knocked down by Tobacco rattle virus (TRV)-based silencing system to inspect the coat protein accumulation after virus inoculation. Results indicate that the coat protein accumulation is reduced about 40% on the knockdown plants to that of control plants. The accumulation levels of viral RNA analyzed in Northern blot also show similar reduction in knockdown plants. However, the coat protein accumulation levels of Potato Virus X (PVX) and Cucumber mosaic virus (CMV) show no significant difference in ACCT8-1 knockdown and control plants. Further, I also find that the coat protein accumulation has no significant difference and a 40% reduction in the protoplasts and systemic levels derived from the knockdown plants, respectively, compared to those of control plants. Finally, I also find that the lesion sizes in the knockdown plants are smaller than those observed in control plants. Overall of these results suggest that ACCT8-1 may involve in viral movements.en_US
dc.description.tableofcontents中文摘要 i Abstrate ii Contents Introduction 1 Bamboo mosaic virus 1 Interaction between host and pathogen 1 Plant innate immune system 2 cDNA- amplified fragment length polymorphism (cDNA-AFLP) 3 Virus induced gene silencing (VIGS) and its application 4 Aim 5 Material and method 6 Construction silencing plasmid 6 Agrobacterium-infiltration and virus inoculation 6 Cloning and sequencing of ACCT8-1 gene 7 Total protein extraction and western blotting analysis 8 Total RNA extraction and northern blotting analysis 9 Semi-quantitative RT-PCR 10 Protoplast isolation and inoculation 10 Analysis of movement of BaMV 11 Results 13 The full-length cDNA of ACCT8-1 is cloned 13 The BaMV coat protein and RNA accumulation level were all decreased in ACCT8-1 knock downed N. benthamiana plants 14 ACCT8-1 is involving in BaMV cell-to-cell movement 15 Discussion 17 Table and Figures 20 Reference 37zh_TW
dc.subjectBamboo mosaic virusen_US
dc.subjectCladosporium resistance geneen_US
dc.subjectLRR proteinen_US
dc.subjectCladosporium fulvum抗病基因zh_TW
dc.subjectLRR 蛋白質zh_TW
dc.titleThe study of the involvement of the host gene ACCT8-1 from Nicotiana benthamiana in the infection cycle of Bamboo mosaic virusen_US
dc.typeThesis and Dissertationzh_TW
item.openairetypeThesis and Dissertation-
item.fulltextno fulltext-
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