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Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/36279
標題: 文心蘭中兩個SVP同源基因OnSVP1/2參與調控花器老化及凋落與開花時間之功能性分析
Functional Analysis of Two Oncidium SVP Orthologues, OnSVP1 and 2, in Regulating Flower Senescence, Abscission and Flowering Time
作者: 謝東霖
Hsieh, Tung-Lin
關鍵字: 文心蘭, 花器老化, 花器掉落;MADS box gene, SVP
出版社: 生物科技學研究所
引用: 吳家偉 (2006) 文心蘭中 B 和 E 功能性之 MADS box 開花基因之選殖與特性分析. 碩士論文. 李佩芳 (2010) 文心蘭與阿拉伯芥中調控花器老化及脫離相關基因之選殖與功能性分析. 碩士論文. 徐杏芬 (2003) 文心蘭花朵發育相關之 MADS box 基因之選殖及功能分析. 博士論文. 高乃萱 (2007) 文心蘭中 B功能性 MADS Box基因之選殖與特性分析. 碩士論文. Adamczyk BJ, Lehti-Shiu MD, Fernandez DE (2007) The MADS domain factors AGL15 and AGL18 act redundantly as repressors of the floral transition in Arabidopsis. Plant J 50: 1007-1019 Ambrose BA, Lerner DR, Ciceri P, Padilla CM, Yanofsky MF, Schmidt RJ (2000) Molecular and genetic analyses of the silky1 gene reveal conservation in floral organ specification between eudicots and monocots. Mol Cell 5: 569-579 Becker A, Theissen G (2003) The major clades of MADS-box genes and their role in the development and evolution of flowering plants. Mol Phylogenet Evol 29: 464-489 Bleecker AB, Patterson SE (1997) Last exit: senescence, abscission, and meristem arrest in Arabidopsis. Plant Cell 9: 1169-1179 Butenko MA, Patterson SE, Grini PE, Stenvik GE, Amundsen SS, Mandal A, Aalen RB (2003) Inflorescence deficient in abscission controls floral organ abscission in Arabidopsis and identifies a novel family of putative ligands in plants. Plant Cell 15: 2296-2307 Cai S, Lashbrook CC (2008) Stamen abscission zone transcriptome profiling reveals new candidates for abscission control: enhanced retention of floral organs in transgenic plants overexpressing Arabidopsis ZINC FINGER PROTEIN2. Plant Physiol 146: 1305-1321 Chen M-K, Hsu W-H, Lee P-F, Thiruvengadam M, Chen H-I, Yang C-H (2011) The MADS box gene, FOREVER YOUNG FLOWER, acts as a repressor controlling floral organ senescence and abscission in Arabidopsis. The Plant Journal: Accepted Chen YF, Etheridge N, Schaller GE (2005) Ethylene signal transduction. Ann Bot 95: 901-915 Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW (2005) AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana. Development 132: 4563-4574 Fernandez DE, Heck GR, Perry SE, Patterson SE, Bleecker AB, Fang SC (2000) The embryo MADS domain factor AGL15 acts postembryonically. Inhibition of perianth senescence and abscission via constitutive expression. Plant Cell 12: 183-198 Fornara F, Gregis V, Pelucchi N, Colombo L, Kater M (2008) The rice StMADS11-like genes OsMADS22 and OsMADS47 cause floral reversions in Arabidopsis without complementing the svp and agl24 mutants. Journal of Experimental Botany 59: 2181-2190 Gonzalez-Carranza ZH, Rompa U, Peters JL, Bhatt AM, Wagstaff C, Stead AD, Roberts JA (2007) Hawaiian skirt: an F-box gene that regulates organ fusion and growth in Arabidopsis. Plant Physiol 144: 1370-1382 Hayes TE, Sengupta P, Cochran BH (1988) The human c-fos serum response factor and the yeast factors GRM/PRTF have related DNA-binding specificities. Genes & Development 2: 1713-1722 He C (2004) On the origin of floral morphological novelties. FEBS Letters 567: 147-151 Hepworth SR, Zhang Y, McKim S, Li X, Haughn GW (2005) BLADE-ON-PETIOLE-dependent signaling controls leaf and floral patterning in Arabidopsis. Plant Cell 17: 1434-1448 Hiratsu K, Matsui K, Koyama T, Ohme-Takagi M (2003) Dominant repression of target genes by chimeric repressors that include the EAR motif, a repression domain, in Arabidopsis. Plant J 34: 733-739 Huang (1993) Isolation and characterization of the binding sequences for the product of the Arabidopsis floral homeotic gene AGAMOUS. Nucleic Acids Research Volume21, Issue20 Pp. 4769-4776 Immink RG, Gadella TW, Jr., Ferrario S, Busscher M, Angenent GC (2002) Analysis of MADS box protein-protein interactions in living plant cells. Proc Natl Acad Sci U S A 99: 2416-2421 Jinn TL, Stone JM, Walker JC (2000) HAESA, an Arabidopsis leucine-rich repeat receptor kinase, controls floral organ abscission. Genes Dev 14: 108-117 Kandasamy MK, Deal RB, McKinney EC, Meagher RB (2005a) Silencing the nuclear actin-related protein AtARP4 in Arabidopsis has multiple effects on plant development, including early flowering and delayed floral senescence. Plant J 41: 845-858 Kandasamy MK, McKinney EC, Deal RB, Meagher RB (2005b) Arabidopsis ARP7 is an essential actin-related protein required for normal embryogenesis, plant architecture, and floral organ abscission. Plant Physiol 138: 2019-2032 Kaufmann K, Melzer R, Theissen G (2005) MIKC-type MADS-domain proteins: structural modularity, protein interactions and network evolution in land plants. Gene 347: 183-198 Kramer EM, Dorit RL, Irish VF (1998) Molecular evolution of genes controlling petal and stamen development: duplication and divergence within the APETALA3 and PISTILLATA MADS-box gene lineages. Genetics 149: 765-783 Lee JH, Park SH, Lee JS, Ahn JH (2007) A conserved role of SHORT VEGETATIVE PHASE (SVP) in controlling flowering time of Brassica plants. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression 1769: 455-461 Li D, Liu C, Shen L, Wu Y, Chen H, Robertson M, Helliwell CA, Ito T, Meyerowitz E, Yu H (2008) A repressor complex governs the integration of flowering signals in Arabidopsis. Dev Cell 15: 110-120 Li Z-M, Zhang J-Z, Mei L, Deng X-X, Hu C-G, Yao J-L (2010) PtSVP, an SVP homolog from trifoliate orange (Poncirus trifoliata L. Raf.), shows seasonal periodicity of meristem determination and affects flower development in transgenic Arabidopsis and tobacco plants. Plant Molecular Biology 74: 129-142 Liu C, Zhou J, Bracha-Drori K, Yalovsky S, Ito T, Yu H (2007) Specification of Arabidopsis floral meristem identity by repression of flowering time genes. Development 134: 1901-1910 Masiero S (2004) INCOMPOSITA: a MADS-box gene controlling prophyll development and floral meristem identity in Antirrhinum. Development 131: 5981-5990 McKim SM, Stenvik GE, Butenko MA, Kristiansen W, Cho SK, Hepworth SR, Aalen RB, Haughn GW (2008) The BLADE-ON-PETIOLE genes are essential for abscission zone formation in Arabidopsis. Development 135: 1537-1546 Norberg M, Holmlund M, Nilsson O (2005) The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs. Development 132: 2203-2213 Okushima Y, Mitina I, Quach HL, Theologis A (2005) AUXIN RESPONSE FACTOR 2 (ARF2): a pleiotropic developmental regulator. Plant J 43: 29-46 Patterson SE, Bleecker AB (2004) Ethylene-dependent and -independent processes associated with floral organ abscission in Arabidopsis. Plant Physiol 134: 194-203 Pellegrini L, Tan S, Richmond TJ (1995) Structure of serum response factor core bound to DNA. Nature 376: 490-498 Riechmann JL, Meyerowitz EM (1997) Determination of floral organ identity by Arabidopsis MADS domain homeotic proteins AP1, AP3, PI, and AG is independent of their DNA-binding specificity. Mol Biol Cell 8: 1243-1259 Riechmann JL, Wang M, Meyerowitz EM (1996) DNA-binding properties of Arabidopsis MADS domain homeotic proteins APETALA1, APETALA3, PISTILLATA and AGAMOUS. Nucleic Acids Res 24: 3134-3141 Roberts JA, Elliott KA, Gonzalez-Carranza ZH (2002) Abscission, dehiscence, and other cell separation processes. Annu Rev Plant Biol 53: 131-158 Schwarz-Sommer Z, Huijser P, Nacken W, Saedler H, Sommer H (1990) Genetic Control of Flower Development by Homeotic Genes in Antirrhinum majus. Science 250: 931-936 Sharrocks and Shore (1995) The ETS-domain transcription factors Elk-i and SAP-1 exhibit differential DNA binding specificities. Nucleic Acids Res. 1995 November 25; 23(22): 4698–4706 Solano R, Stepanova A, Chao Q, Ecker JR (1998) Nuclear events in ethylene signaling: a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1. Genes Dev 12: 3703-3714 Stepanova AN, Alonso JM (2005) Arabidopsis ethylene signaling pathway. Sci STKE 2005: cm4 Theissen G (2001) Development of floral organ identity: stories from the MADS house. Curr Opin Plant Biol 4: 75-85 Theissen G, Becker A, Di Rosa A, Kanno A, Kim JT, Munster T, Winter KU, Saedler H (2000) A short history of MADS-box genes in plants. Plant Mol Biol 42: 115-149 Theissen G, Kim JT, Saedler H (1996) Classification and phylogeny of the MADS-box multigene family suggest defined roles of MADS-box gene subfamilies in the morphological evolution of eukaryotes. J Mol Evol 43: 484-516 Theissen G, Saedler H (1995) MADS-box genes in plant ontogeny and phylogeny: Haeckel''s ''biogenetic law'' revisited. Curr Opin Genet Dev 5: 628-639 Theissen G, Saedler H (2001) Plant biology. Floral quartets. Nature 409: 469-471 Trevaskis B, Tadege M, Hemming MN, Peacock WJ, Dennis ES, Sheldon C (2006) Short Vegetative Phase-Like MADS-Box Genes Inhibit Floral Meristem Identity in Barley. Plant Physiology 143: 225-235 Tzeng TY, Liu HC, Yang CH (2004) The C-terminal sequence of LMADS1 is essential for the formation of homodimers for B function proteins. J Biol Chem 279: 10747-10755 Whipple CJ, Ciceri P, Padilla CM, Ambrose BA, Bandong SL, Schmidt RJ (2004) Conservation of B-class floral homeotic gene function between maize and Arabidopsis. Development 131: 6083-6091 Yant L, Mathieu J, Schmid M (2009) Just say no: floral repressors help Arabidopsis bide the time. Current Opinion in Plant Biology 12: 580-586
摘要: 
如何延長文心蘭花朵的瓶插時間是實驗室有興趣的研究。研究中指出異位表現阿拉伯芥中 MADS box genes FOREVER YOUNG FLOWER (FYF) 與 SHORT VEGETATIVE PHASE (SVP) 的轉基因植物有明顯花朵延遲衰老與掉落的現象。為了找到更多調控花朵衰老及掉落的基因，OnSVP1 及 OnSVP2，兩個跟阿拉伯芥 SVP 同源的基因從文心蘭 454 cDNA library 中被選殖出來。為了探討 OnSVP1 以及 OnSVP2 的基因功能，將構築好的35S::OnSVP1 以及 35S::OnSVP2 基因載體轉入阿拉伯芥中。35S::OnSVP1 以及 35S::OnSVP2 轉基因植物的開花時間被延遲，下游 FT 基因也受到抑制。另外，在互補實驗中轉基因植物開花時間延遲，所以 35S::OnSVP1/2 挽救了部份 SVP 的功能。可是在 35S::OnSVP1/2 轉基因植物中並沒有看到花朵的延遲衰老與掉落。然而，將一個很強的 repressor domain SRDX 接上 OnSVP1 後，轉殖植物的開花時間延遲而且花朵的衰老與掉落也有延遲。這個結果指出在文心蘭中 OnSVP1/2 以抑制者的角色控制花朵的老化以及掉落。將 35S::OnSVP1/2 轉基因植物以乙烯處理之後，延遲花器老化與脫離的性狀並沒有消失，顯示 OnSVP1/2 可能影響乙烯的訊息傳遞。經由 real-time PCR 的結果顯示，乙烯訊息傳遞的部份基因，如 ETR2、ERS2 與 EDF1 在 35S::OnSVP1/2 轉基因植物中被抑制；花器老化與脫離相關基因中，在35S::OnSVP1/2 轉基因植物中沒有被明顯的抑制。本實驗結果顯示，OnSVP1/2 經由抑制乙烯的訊息傳遞，因此控制花器的老化與脫離。未來，對 OnSVP1/2 在文心蘭裡專一的運用可以控制文心蘭花朵的保鮮期，進而增加文心蘭的市場價值與農業的品質。

The genes related to prolong the time for Oncidium flowers to stay fresh is of interest in laboratory. It has been reported that ectopic expression of Arabidopsis MADS box genes FOREVER YOUNG FLOWER (FYF) and SHORT VEGETATIVE PHASE (SVP) caused significantly delayed flower senescence and abscission in transgenic Arabidopsis. To look for more genes regulating flower senescence and abscission, two orthologues of Arabidopsis SVP, OnSVP1 and OnSVP2, were identified from Oncidium 454 library. To investigate the function of OnSVP1 and OnSVP2, 35S::OnSVP1 and 35S::OnSVP2 were transformed into Arabidopsis. Flowering time was delayed and the expression of downstream gene FT was repressed in 35S::OnSVP1/2 transgenic plants. In addition, 35S::OnSVP1/2 rescued part of SVP function in svp mutant by delayed flowering in transgenic plants. However, delayed flower senescence and abscission were not seen in 35S::OnSVP1/2 plants. Nevertheless, once OnSVP1/2 was fused with a strong repressor domain SRDX, delayed flower senescence and abscission was observed. This result indicated that OnSVP1/2 possibly act as repressors controlling floral organ senescence and abscission. The delayed senescence and abscission of the flower organs in 35S::OnSVP1/2 Arabidopsis was unaffected by the ethylene treatment. Downstream genes of ethylene signaling pathway such as ETR2, ERS2 and EDF1 were repressed in 35S::OnSVP1/2 transgenic Arabidopsis. However, the expression of senescence/abscission associated genes was not significantly affected in 35S::OnSVP1/2 flowers. Data suggested a novel role for MADS box gene OnSVP1/2 in controlling the floral senescence and abscission by regulating downstream of ethylene perception. In the future, the specific manipulation of OnSVP1/2 in Oncidium for controlling the shelf life of flowers would increase its market value as well as improve the quality of agriculture.
URI: http://hdl.handle.net/11455/36279
其他識別: U0005-1506201114252700
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