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標題: 丹參酚酸鎂鹽B活血化瘀與樟芝酸A抗發炎之分子機制探討
Molecular Mechanisms of Magnesium Lithospermate B and Antcin A in Promoting Blood Circulation and Anti-inflammation
作者: 陳怡菁
Chen, Yi-Ching
關鍵字: Danshen;丹參;MLB;Niuchangchih;Antrodia camphorata;Taiwanofungus camphoratus;Antcin A;molecular mechanism;anti-inflammation;calcium;glucocorticoid;cardiac glycosides;牛樟芝;丹參酚酸鎂鹽B;樟芝酸A;活血化瘀;抗發炎;機制;鈣離子;醣皮質激素;強心配醣體
出版社: 生物科技學研究所
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植物藥已經被作為中草藥的來源,從過去到現在持續的作為許多藥物的使用依據。本論文的研究主題,是研究丹參酚酸鎂鹽B (MLB) 活血化瘀與樟芝酸A (antcin A) 抗發炎的分子機制。過去的研究顯示,丹參酚酸鎂鹽B能夠抑制鈉鉀幫浦;本實驗結果顯示,丹參酚酸鎂鹽B與哇巴因 (ouabain, 強心配醣體) 相同,都能夠使神經細胞內的鈣離子增加;而鈣離子的來源是藉由鈉鈣交換蛋白,將細胞外的鈣離子送入細胞內,以及內質網膜上的IP3通道,將鈣離子釋放至細胞質中;利用分子模擬分析顯示,丹參酚酸鎂鹽B與哇巴因,均可與鈉鉀幫浦形成五個氫鍵;丹參酚酸鎂鹽B並不會使細胞的形態有所改變,綜合上述結果,丹參酚酸鎂鹽B與哇巴因相同,皆具有抑制鈉鉀幫浦的能力並啟動下游相同的機制,因此,丹參酚酸鎂鹽B具有潛力,開發作為心臟疾病的治療用藥。

Botanical remedies have been used as a source of traditional Chinese medicines throughout history and continue to serve as the basis for many pharmaceuticals used today. This study focused on the molecular mechanism of magnesium lithospermate B (MLB) and antcin A in the promotion of blood circulation and anti-inflammation. Previous studies have indicated that the MLB extract from the Danshen (Salvia miltiorrhiza Bunge) inhibits the activity of Na+/K+-ATPase. The results of Chapter 1 demonstrate that the increase in intracellular free Ca2+ levels follows similar patterns when MLB and ouabain were used to treat neuroblastoma cells. Intracellular free Ca2+ is obtained from outside the cell via the Na+/Ca2+ exchanger and IP3 channel of the ER membrane. Both MLB and ouabain are equivalent, forming 5 hydrogen bonds in the Na+/K+-ATPase cavity. There was a change in dendrite morphology under ouabain treatment, but not under MLB. Comparable to ouabain, MLB triggered the same mechanism by inhibiting Na+/K+-ATPase, and it possesses more potential as a botanical medicine for cardiac disease.
Niuchangchih (Antrodia camphorata, syn. Taiwanofungus camphoratus) is a unique medicinal fungus found in Taiwan. Its anti-inflammatory effect has been reported, and consumer experience attests to this. The results in Chapter 2 demonstrate that the structure of antcin A, extracted from the fruiting bodies of Niuchangchih, is similar to that of the glucocorticoid cortisone. Examination of the nuclear migration of the glucocorticoid receptor (GR) effect demonstrated that antcin A and glucocorticoids both migrate into the nucleus and the minimal effective concentration of antcin A exceeds that of glucocorticoids. Molecular modeling indicated that antcin A forms hydrogen bonds less than the glucocorticoid dexamethasone. In the study, the anti-inflammatory effect of Niuchangchih should be partly attributed to antcin A, which mimics glucocorticoids and triggers translocation of the GR into the nucleus to initiate suppression of inflammation via gene regulation.
其他識別: U0005-2604201123555000
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