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標題: 鎳-黏土成長奈米碳管於電化學生物感測器之應用
Applications of carbon nanotubes synthesized over the Ni particles on clay for Electrochemical biosensor
作者: 劉又禎
Liu, You-Chen
關鍵字: biosensors;生物感測器;clay;carbon nanotubes;黏土;奈米碳管
出版社: 化學工程學系所
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本研究目的為製備奈米碳管-黏土/Nafion 薄膜修飾玻璃碳電極,並將電極應用於偵測過氧化氫。在pH=7 的磷酸緩衝溶液下,奈米碳管-黏土/Nafion 薄膜修飾玻璃碳電極的操作電壓為-0.6V。當過氧化氫累積濃度為12.8mM 時,奈米碳管-黏土/Nafion 薄膜修飾玻璃碳電極能產生較高的電流為136.87μA。且在掃描速率為25 到200mV/s,整個電催化過程為表面控制。
而奈米碳管-黏土/Nafion 薄膜修飾玻璃碳電極, 在含有8mg/ml 的
CNTs-Clay 量,和取6μl的CNTs-Clay/Nafion 溶液以及掃描速率為200rpm的最佳條件下,其靈敏度為10942 nA/mM,應答時間為5 秒,偵測範圍為0.1~12.8mM 的過氧化氫。
在葡萄糖偵測方面,當葡萄糖氧化酵素濃度為2mg/ml 時,靈敏度顯
示為1718nA/mM,線性範圍0.2~1.3mM,應答時間為10 秒,而葡萄糖濃度在2.8mM 時,應答電流為3.02μA。

The preparation of biosensors based on the carbon nanotubes (CNTs)-Clay/Nafion coated on the thin film glassy carbon electrode (GCE) fordetecting the hydrogen peroxide (H2O2) was investigated. The CNTs-Clay/Nafion with a operating potential of -0.6 V in 0.1 M, pH 7.0
phosphate buffer solution. The superior performance of higher current of 136.87 μA with the accumulation of 12.8mM H2O2 was observed as the CNTs-Clay/Nafion modified glassy carbon electrode. The response has shown
a surface-controlled electrocatalyze process determined in the scan rate range from 25 to 200 mV/s.
Under the optimal conditions of 8mg/ml CNTs-Clay, 6μl
CNTs-Clay/Nafion solution, pH 7.0 phosphate buffer solution and agitation speed of 200rpm, the sensitivity and response time of the CNTs-Clay/Nafion/GCE biosensor were 10942 nA/mM and 5 seconds,respectively, in the detecting range of 0.1~12.8mM H2O2 .
The resulting sensor displays a high sensitivity (1718nA/mM) and a linear range from 0.2 to 1.3 mM, the response time was 10 seconds for glucose determination, the current of 3.02 μA with the accumulation of 2.8mM
Key words: biosensors, clay, carbon nanotubes
其他識別: U0005-1607200715565000
Appears in Collections:化學工程學系所

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