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標題: NTMG (N-terminal Truncated Mutants Generator for cDNA): an automatic multiplex PCR assays design for generating various N-terminal truncated cDNA mutants
作者: Chen, Y.F.
Chen, R.C.
Tseng, L.Y.
Lin, E.
Chan, Y.K.
Pan, R.H.
關鍵字: polymerase chain-reaction;primer design;program
Project: Nucleic Acids Research
期刊/報告no:: Nucleic Acids Research, Volume 35, Page(s) W66-W70.
The sequential deletion method is generally used to locate the functional domain of a protein. With this method, in order to find the various N-terminal truncated mutants, researchers have to investigate the ATG-like codons, to design various multiplex polymerase chain reaction (PCR) forward primers and to do several PCR experiments. This web server (N-terminal Truncated Mutants Generator for cDNA) will automatically generate groups of forward PCR primers and the corresponding reverse PCR primers that can be used in a single batch of a multiplex PCR experiment to extract the various N-terminal truncated mutants. This saves much time and money for those who use the sequential deletion method in their research. This server is available at
ISSN: 0305-1048
DOI: 10.1093/nar/gkm305
Appears in Collections:資訊網路與多媒體研究所

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