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標題: 聚乳酸聚甘醇酸奈米陶瓷複合材料於骨再生之應用
The application of poly(lactic-co-glycolic acid) nanocomposites in bone regeneration
作者: 郭明瑞
Kuo, Ming-Jui
關鍵字: Air plasma;大氣電漿;PLGA;nCDHA;CBD-RGD;聚乳酸聚甘醇酸;缺鈣氫氧基磷灰石;CBD-RGD
出版社: 化學工程學系所
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本研究分為兩部份探討,第一部份為混摻不同比例50、100、500及1000 ppm之缺鈣氫氧基磷灰石(nano-calcium deficient
Hydroxyapatite;nCDHA)於Poly-(lactide-co-glycolide) (PLGA)溶液中製成薄膜材料,培養老鼠骨髓基質幹細胞(BMSC)於材料上,探討在各濃度之細胞相容性及基因表現。由本研究結果發現混摻不同比例50、100、500及1000 ppm奈米陶瓷之材料表面顯示粒徑隨濃度漸變大然後再變小,且由細胞相容性實驗發現,細胞貼附與表面奈米粒徑大小有關,然而在50 ppm有較好之細胞貼附和ALP、OCN基因表現量,且能維持ALP表現量至14天。第二部份為將第一部份所找出最佳濃度50 ppm之PLGA奈米複合材料,利用大氣電漿接枝上重組之貼附蛋白質(cellulose-binding domain-arginine-glycine-aspartic acid;CBD-RGD),由ESCA分析顯示N基在接枝RGD後增加且C-N鍵亦增加,再由接枝後之接觸角上升可證明CBD-RGD成功接枝在材料表面,且接枝上CBD-RGD之PLGA有較好之細胞貼附及ALP基因表現。由以上述可得知,奈米陶瓷不僅能促進BMSC細胞貼附且能維持基因表現量,然而大氣電漿是強大且方便之工具,電漿改質後不僅能促進細胞貼附且能有效接枝CBD-RGD於PLGA表面。

This research includes two different modification of

poly-(lactide-glycolide) (PLGA). In the first part, PLGA films were

mixed with different nano-calcium deficient hydroxyapatite (nCDHA) in

a concentrative 50-1000 ppm. Cell proliferation and gene expression of

ALP and OCN of bone marrow stromal cells (BMSC) were enchanced by

the nonocomposite. Moreover, the osteoinductivity of PLGA-nCDHA

composite kept ALP gene expression of the cells at 14 days. 50 ppm of

nCDHA was the aptconcentration. In the second part, cellulose-binding

domain arginine-glycine-aspartic acid (CBD-RGD) was successfully

grafted onto the surface of PLGA by air plasma, confirmed by ESCA

data. CBD-RGD also enhanced the adhesion and ALP gene expression of

BMSC. It was concluded from this study that the use of air plasma was a

powerful technique for modification of biomaterials, and nCDHA

combined with CBD-RGD were conducive to bone differentiation of

其他識別: U0005-3001200820125200
Appears in Collections:化學工程學系所

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