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Effects of Selenium Priming Treatments on Glutathione Peroxidase Activity of Bitter Gourd Seeds
Lin, Yung Kang
|關鍵字:||selenium;硒;priming;glutathione peroxidase;bitter gourd;萌爆處理;穀胱過氧化;苦瓜||出版社:||農藝學系||摘要:||
Selenium is a constitute of glutathione peroxidase (GPX). It is kown that priming the seeds with selenium solution enhances the activity of seed's GPX. In the study, bitter gourd seeds (cv. Moon Shine) were primed with water or 5 ppm selenium solution, the activities of GPX and antioxidants were determined during hydration and redrying period. The results indicated that the embryo axis GPX activity was enhanced by selenium priming. Additionally selenium priming was increase the glutathione contents of treated seeds. For further examing the effect of selenium on the expression of GPX, we designed a degenerate primer to clone the GPX genes of bitter gourd seeds. The nucleotide sequence of cloned GPX300-6 matched to the GPX of other published species using NCBI Blast analysis. Genomic southern analysis showed that the GPX300-6 exits a single copy gene in Moon Shine bitter gourd seed. According to the nucleotide sequence of GPX, we designed the specific primer of GPX, and using PCR strategy to analyse the expression of GPX during priming. And it appears that GPX was expressing during both priming treatments. The expression of GPX was higher in the stage of desiccation, as compared with the GPX expressed at hydration.
|Appears in Collections:||農藝學系|
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