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Seed vigor testing in rice
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Relationships between seed respiration during imbibition and subsequent seedling growth in Zea mays L. Plant Physiol. 42: 1071-1076. Woodstock, L. W., K. Furman, and H. R. Leffer. 1985. Relationship between weathering deterioration and germination, respiration metabolism, and mineral leaching from cottonseeds. Crop Sci. 25: 459-466. Yu, M. Q., and S. Q. Song. 2008. Early morphological and physiological events occurring during germination of maize seeds. Agricultural Sciences in China 7: 950-957. Zhao, G., L. Yang, J. Wang,and Z. Zhu. 2009. In “IFIP International Federation for Information Processing”, eds. D. Li, Z. Chunjiang, Boston: Springer. pp. 1729–1738.||摘要:||
台灣稻種繁殖主要透過「三級繁殖制度」倍增種子量，為確保稻種品質，針對各級繁殖田進行田間和室內檢查的工作。其中稻種之標準發芽率檢測需耗時14天，然而一、二期作交接期間可供稻種調製時間極短，故須發展出能快速、簡便且客觀判別種子活力的方法，為此本研究以兩個方向進行試驗，其一為評估提早檢測稻種活力之可能性，透過觀測稻穀生育期間發芽率變化，發現於糊熟期(23 ~ 29 DAF)時即可檢測稻種之標準發芽率；其二為找尋能準確判別稻種活力之快速活力檢測法，所使用之檢測法包括四唑鹽法、電導度法和呼吸速率法，結果顯示以呼吸速率法能準確且快速判別稻種活力。
In Taiwan, the production of rice seed is mainly through "three-step propagation system". To ensure genetic purity and quality of seed to the seed grower, the seed certification system that is overseen by the government seed certification agencies. Seed-certified inspector will conduct field and seed inspection for each seed classes. One of the major item of seed inspection is germination test. According to the germination rules of the International Seed Testing Association (ISTA), the standard germination takes at least 14 days. However the transition period between the first and the second crop seasons is very short in Taiwan. It must develop a rapid, simple and objective methods to evaluate seed vigor. In this study, we investigated seed standard germination, tetrazolium test, membrane integrity status, and respiration rate in seven rice cultivars (Taikong 8, Taikong 9, Taikong 14, Tannan 11, Tainung 71, Kaohsiung 145, and Taitong 30) during different stages of seed development and early imbibition. The results indicated that the standard germination test can be conducted during the dough stage (23 ~ 29 DAF) to assess the potential of seed vigor. Tetrazolium test, conductivity and respiration rate can provide the useful index in each seed lot. Results demonstrated that respiration rate test is a reliable, simple, and rapid index to determinate the germinability of rice grain.
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