Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/36772
標題: 菘藍無性繁殖之研究
Studies of the vegetative propagation of Isatis indigotica Fortune
作者: 賴建宏
Lai, Chien-Hung
關鍵字: Isatis indigotica Fortune;菘藍;vegetative propagation;tissue culture;cutting;hyperhydricity;無性繁殖;組織培養;扦插;玻璃質化
出版社: 農藝學系所
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摘要: 
本試驗以菘藍(Isatis indigotica Fortune)之葉片與根為材料,探討(1)不同生長調節劑對於不定芽體誘導、增殖及植株再生之影響;(2)根插穗種類與扦插方式對於側生苗數與插穗存活率之影響;(3)組織培養與扦插繁殖之效益比較。試驗結果摘述如下:
根據結果發現,幼葉之褐化率較成熟葉低,且分化能力亦比成熟葉強,幼葉之葉身、中肋及葉柄等3種培植體中,以葉身之褐化率最低、芽體形成率最高,為最適合的組織培養材料。5種植物生長調節劑(IAA、NAA、BA、kinetin及TDZ)均可誘導不定芽形成,並以1 mg/l TDZ之芽體形成率達96.67%為最高,芽體數11.7個為最多。此外,除了MS基礎培養基以外,添加生長調節劑(IAA、NAA、BA、kinetin及TDZ)均會產生玻璃質化的不定芽。MS基礎培養基添加2 mg/l NAA之芽體發根率達70%,可形成5.7條不定根。泥炭土混合珍珠石為較佳之馴化栽培介質,並以生長箱為較適合的馴化環境。
比較不同的扦插方式,發現長粗根插穗較適合水平插,長細根插穗較適合斜插。插穗斜插之存活率較水平插高,其中又以正向斜插可獲得較高之存活率與較多側生苗數。
菘藍以根段扦插法再生植株僅需56天,而且有成本低及無芽體玻璃質化等優點,應為菘藍較適合的無性繁殖方式。

In this study, Isatis indigotica Fortune was applied to investigate: 1. Effects of growth regulators (auxin and cytokinin) on the induction, propagation of adventitious bud, and further plant regeneration. 2. The influence of various root cutting and cutting methods on shoot propagation. 3. The propagation efficiency of tissue culture and root cutting was compared. The young leaf was the best explant resulting high rate of shoot formation, shoot number, and the lowest browning rate. The optimum condition was obtained by young leaf explants supplemented with TDZ. Except the MS medium alone treatment, the adding of any plant growth regulators (IAA, NAA, BA, kinetin, and TDZ) caused hyperhydric shoots. MS based medium contained 2 mg/l NAA leading to the highest rooting rate with 6.3 roots per shoot. Mixture with equal amount of peat and perlite was the most suitable medium for plantlet acclimatization.
In consideration of survival rate and lateral shoot number, the cutting with long-thick-root was the best, followed with the long-thin-root one. Of various cutting methods, the slant insertion resulted in a higher survival rate and the straight insertion gave the highest lateral shoots. Therefore, the using of long-thin-root cuttings and slant insertion will lead to high propagation efficiency. In conclusion, a method took only 56 days from root cutting to plantlet with low cost, high efficiency, and suitable for vegetative propagation of Isatis indigotica Fortune was developed.
URI: http://hdl.handle.net/11455/36772
其他識別: U0005-0502200718072300
Appears in Collections:農藝學系

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