Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/36795
標題: 台灣脈葉蘭屬植物族群之遺傳變異、抗氧化能力及腺苷含量之分析
Studies on the Genetic Variation, Antioxidative Activity, and Adenosine Content of Nervilia spp. in Taiwan
作者: 陳振義
Chen, Chen-I
關鍵字: Nervilia;遺傳變異;Genetic variation;Antioxidative activity;Adenosine;抗氧化能力;腺苷
出版社: 農藝學系所
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摘要: 
中文摘要

本研究以收集自台灣各地區的五個脈葉蘭物種共計56個採集系為材料,探討台灣脈葉蘭屬植物之性狀變異、成分分析以及利用inter-simple-sequence-repeat(ISSR)技術探討其遺傳變異與親緣關係,試驗結果敘述如下:
採集五個脈葉蘭物種共計56個採集系,調查13個植物性狀進行分析,結果五個物種間及56個採集系間13個植物性狀都呈顯著性差異;紫花脈葉蘭19個採集系間13個植物性狀除了開花日數無顯著差異外,其餘性狀都呈顯著性差異;東亞脈葉蘭20個採集系間13個植物性狀除了開花日數無顯著差異外,其餘性狀都呈顯著性差異;單花脈葉蘭13個採集系間13個植物性狀都呈顯著性差異;蘭嶼脈葉蘭與古式脈葉蘭各2個採集系間13個植物性狀都無顯著差異。
分析原生脈葉蘭屬植物有效成分腺苷(adenosine)含量之變化,將各地區脈葉蘭屬植物區分為三個不同生長時期,比較不同生長時期植株內腺苷含量之變化,全株腺苷含量最高為嘉義之東亞脈葉蘭(5)第Ⅲ時期含量為0.18%最高,為冬蟲夏草(Cordyceps sinensis)腺苷含量0.03%之6倍,而台灣脈葉蘭各物種腺苷含量皆高實具利用價值。
其次進行台灣原生脈葉蘭屬植物中常為民間所利用之之紫花脈葉蘭及東亞脈葉蘭植物抗氧化能力分析。結果紫花脈葉蘭的酚類化合物含量較東亞脈葉蘭高,其清除含氧自由基如超氧陰離子、氫氧自由基、過氧化氫的能力等皆高於東亞脈葉蘭,對脂質過氧化的抑制作用結果,紫花脈葉蘭清除含氧自由基的能力優於東亞脈葉蘭。
將各地區收集之56個採集系進行ISSR分析,選出具有多型性且訊號較強之10個核酸引子共得到152個條帶,共可得到92個多型性條帶,每一引子平均可得多型性條帶之比例為60.5%,再由92個多型性條帶中選出訊號較強且穩定之30個多型性條帶作為分子標誌。遺傳距離介於0-0.986之間,顯示族群間有很大的遺傳變異。利用分群分析,可分為6大類群,其結果大致能與調查之外表植物性狀能相符合。

Summary

The purpose of this study was to collect 56 lines of five Nervilia species native in Taiwan to investigate their variations of morphological characters, components analysis. Genetic variations and relationships of these 56 lines were also studied by using ISSR. The results were summarized as follows:
Significant differences were found among these five Nervilia species for identifying 13 morphological characters in these 56 lines. Except flowering day (FD), both N. plicata and N. aragoana exhibited significant difference among the other 12 morphological characters in 19 lines and 20 lines. No significant difference was found among 13 morphological characters in 13 lines of N. plicata. No significant differences were found among 13 morphological characters in 2 lines of N. lanyuensis and N. cumberlegei.
Another purpose of this study was to analyze the content of adenosine, a physiological activity compound in Nevilia spp., the whole plants of N. aragoana, native in Chiayi were found to have the highest adenosine content (0.18%) during the growing stage Ⅲ. The adenosine content in N. aragoana (1.8%) was six times of adenosine content higher than that in Cordyceps sinensis (0.03%). All the Nevilia species, native in Taiwan, had high adenosine content and high potential valuable for medicinal use.
Autioxidative capacity on N. plicata and N. aragoana species was used to conduct component analysis . The results showed that phenolic acid content, scavenging effect of superoxide anions, OH free radical and H2O2 ability of N. plicata were found to be better than those of N. aragoana.
From primary ISSR analysis of 56 lines in these five Nervilia species, 92 polymorphic bands from 152 bands in total with 92 valid primers (about 60.5%) were gathered. About 30 valid primers (with highly polymorphic ratio) were chosen from the former 92 ones for the subsequent ISSR analysis. The similarity coefficient of populations, from 0 to 0.986, indicated populations had greater genetic variation. Six clusters were classified according to cluster analysis. The results from the ISSR analysis were similar to those of the morphological characters.
URI: http://hdl.handle.net/11455/36795
其他識別: U0005-1608200608203700
Appears in Collections:農藝學系

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