Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/36801
標題: 黃耆組織培養根誘導與成分分析
Studies on Root Induction in vitro and Composition analysis of Huangqi ( Astragali Radix )
作者: 江嘉偉
Jiang, Jia-Wei
關鍵字: Huangqi;黃耆;Root Induction;in vitro;組織培養;成分分析
出版社: 農藝學系所
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摘要: 
利用蒙古黃耆( Astragalus mongholicus )種子試管內無菌播種後60天,以根系生長較佳的植株為材料,探討組織培養方式的芽體形成與試管內扦插發根,以建立試管內種原、優良單株大量繁殖,進一步進行田間栽培試驗,並比較組培苗與市售商品在主要成分方面之差異。

試驗發現,在誘導芽體培養基,以2/3 MS、3% sucrose為基礎培養基,添加25 μg/l BA對誘導芽體增殖效果佳,培養3週平均每個培植體可誘導2.7個芽體,且植株整體白化、玻璃質化情形輕微,而在一年半後的連續3次增殖試驗中,每個培植體仍可維持平均2.8個芽體,結果顯示流程穩定性良好。

試管內誘導發根方面,以1/6 MS、1-1.5 % sucrose及0.3 mg/l NAA培養基,添加0-25 μg/l BA和20-40 mg/l ascorbic acid可達到誘導發根最佳條件,培養6週後,培植體平均可達到70%發根率。在植株白化方面,培養基中添加20-40 mg/l ascorbic acid有顯著抑制白化現象。

發根植株馴化出瓶方面,以真珠石和泥碳土(1:1)的栽培介質,配合出瓶種植前透氣一星期,及浸泡殺菌劑處理,出瓶存活率可達到61-76%,而後在田間的存活率亦高達90%以上,從出瓶馴化到田間栽培,均可維持良好的存活率。

組培苗與市售商品在黃耆皂苷IV ( astragaloside IV )成分之間差異,經HPLC分析之後,發現部分種植於田間的組培苗,其astragaloside IV成分比市售黃耆藥材、順天堂黃耆濃縮散及勝昌濃縮黃耆粉高出4-10倍,證明本研究發展之流程獲得的部分植株之有效成分和市售黃耆比較結果,為具有更高的astragaloside IV成分的優良單株。

Seeds of Astragalus mongholicus were sowed sterilely for 60 days and the elite plants with vigorous root growth in vitro were selected. Studies on multi-bud induction and then root induction from buds of elite plant and further cultivation in field were conducted. Finally, the contents of astragaloside IV of roots mentioned above were analyzed.
The best response of multi-bud proliferation was the induction treatment with medium containing 2/3 MS, 3% sucrose, 25 μg/l BA and 20-40 mg/l ascorbic acid. It had 2.7 buds averagely per explant for 3-week induction treatment. Chlorosis at tips and hyperhydration of plantlets occurred slightly. After one and half years, the buds were then subcultured three times continually with the same medium and there were 2.8 buds averagely per explant of subcultures. The results showed that multi-bud running protocol was reliable.
For root induction, the medium containing 1/6 MS, 1-1.5% sucrose 0.3 mg/l NAA and 0-25 μg/l BA had the best response of root induction. There were 70% rooted plantlets inducted for 6-week cultures. In addition, the root induction medium containing additional 20-40 mg/l ascorbic acid reduced chlorosis at tip of plantlets.
Seedling survival rate could reach 61-76% by disposing the seedlings derived from rooting culture with allowed ventilation gradually persisted for one week and transplanted in the mixture ( peat moss : perlite = 1:1 v/v ). These seedlings were then transplanted in field and the survival rate was more than 90%.
The results of analysis of astragaloside IV of different roots as mentioned showed that some plants derived from tissue culture with higher astragaloside IV content 4 to 10 times than those collected from market, Sun-Ten astragali extract powder and Sheng-Chang astragali extract powder. The protocol developed for A. mongholicus plant, here, indicates that it is possible to select elite plants with high astragaloside IV content.
URI: http://hdl.handle.net/11455/36801
其他識別: U0005-1907200617370600
Appears in Collections:農藝學系

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