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標題: 鹽分逆境下水稻幼苗氧化傷害及glutathione和ascorbate反應的探討
Oxidative damage and responses of glutathione and ascorbate in rice (Oryza sativa L.) seedlings subjected salinity stress.
作者: 許慈婷
Syu, Cih-Ting
關鍵字: salinity stress;鹽分逆境;rice;oxidative damage;水稻;氧化傷害
出版社: 農藝學系所
引用: 朱德民。2002。作物耐旱原動力-滲透調節物。p.97-104。農業氣象及農業水資源之應用與管理研討會。中華民國農業氣象學會。台北。 林傳琦、高景輝。2000。細胞壁與鹽分逆境所抑制之生長。科學農業48:169-175。 Alves, A. C., and T. L. Setter. 2004. Abscisic acid accumulation and osmotic adjustment in cassava under water deficit. Environ. Exp. Bot. 51:259-271. Ashraf, M., and P. J. C. Harris. 2004. Potential biochemical indicators of salinity tolerance in plant. Plant Sci. 166:3-16. Bohnert, H. J., and B. Shen. 1999. Transformation and compatible solutes. Sci. Horti. 78: 237-260. Bor, M., F.
本試驗目的是探討水稻幼苗葉片於鹽分逆境下氧化傷害(oxidative damage)程度及穀胱

The purpose of this study is to explore oxidative damage levels, the changes of total glutathione and ascorbate (AsA) contents and it's play roles in rice seedlings subjected salinity stress. In addition, the possibility of decrease oxidative damages of plant and promote salt tolerance by either osmolytes or abscisic acid (ABA) pretreatments under salinity stress. Intact leaves and detached leaves of rice cultivar TNG 67 seedlings at 3-leaf stage were used as materials in this study, the experiment results were summarized as below:
Rice seedlings were subjected to NaCl for 3d, chlorophyll content was decreased; enhancement of hydrogen peroxide (H2O2) and malondialhehyde (MDA) contents were accompanied with increasing salt concentration in plants, suggested oxidative damage more serious; the contents of soluble protein had no change, total glutathione content was increased, and AsA content was decreased, particularly in high salt concentration.
The contents of soluble protein, chlorophyll, H2O2 and MDA content were no significantly changes in rice detached leaves treated NaCl for 36h, total glutathione content was increased, however content of AsA showed decreasing trend as increasing NaCl concentration. The content of total glutathione and MDA was significantly increased by 240 mM NaCl treatment, especially. The reaction of intact seedling leaves and detached leaves were similar under different concentrations of NaCl, but the reaction levels of detached leaves lower than intact seedling. Reaction of detached leaves was only significant at high NaCl concentration treatment.
The contents of H2O2, MDA and total glutathione were increasing, but AsA content was decreasing as gradually increasing NaCl concentration in rice seedling. The results also showed the similar reactions occurance as increasing treating time at the same NaCl concentration. It is evident that adjust osmotic potential in plant with gradually increasing NaCl concentration can't decrease the damage levels in plant under salinity stress.
The rice detached leaves were pretreated with three concentrations of glucose for 12h and then transferred to 180 mM NaCl for 36h. Oxidative damage levels were decreased in detached leaves by medium (0.1 M) glucose pretreatment under salinity stress, due to the content of total glutathione was significantly increased and maintained the content of AsA.
Reactions of rice leaves were pretreated with different osmolytes and then transferred to NaCl were not identical completely. Generally, the contents of H2O2 and AsA were decreasing, the content of MDA was no effect and total glutathione content was significantly increasing by all osmolytes pretreatment in salinity stress. The results showed the effect of oxidative damages was decreased by sucrose more than proline, sorbitol and NaCl pretreatment respectively.
The detached leaves were pretreated with ABA and ABA biosynthesis inhibitor, tungstate and ABA+ tungstate. The decreased MDA content and increased content of total glutathione were the most in detaced leaves was pretreated with tungstate. Threrfore, effects of oxidative damage were mitigated by tungstate pretreatment under salinity stress was best; effects of ABA and ABA+ tungstate pretreatment were not good. The results of ABA, tungstate and ABA+ tungstate pretreatment were not conformed to our expectation. In other words, it was not have the effect of oxidative damage was mitigated by ABA pretreatment in this experiment. The possible causes were discussed in this paper.
其他識別: U0005-2006200610294000
Appears in Collections:農藝學系

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