Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/36814
標題: 大薊與小薊組織培養之研究
Mass Propagation of Cirsium japonicum DC. and Cirsium albescens Kitamura by Tissue Culture
作者: 陳景明
Chen, Ching-Ming
關鍵字: 大薊;Cirsium japonicum DC.;小薊;薊屬;組織培養;染色體數;Cirsium albescens Kitamura;Cirsium;Tissue culture;Chromosome number
出版社: 農藝學系所
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摘要: 
本試驗探討大薊(Cirsium japonicum DC.)與小薊(Cirsium albescens Kitamura)利用組織培養植株之不同部位培植體再生大量繁殖之條件,並觀察染色體數是否產生變異,期能建立大薊與小薊組織培養快速繁殖技術,作為大量繁殖與栽培之參考。試驗結果摘錄如下:
以大薊與小薊之莖節、葉片和管狀小花為培植體,其中大薊與小薊莖節和葉片培養於含有0.2 mg/L BA與0.1 mg/L NAA組合處理之MS固體基本培養基照光處理可獲得大量增殖芽體;大薊管狀小花以1.0 mg/L BA與0.1 mg/L NAA和4.0 mg/L BA與0.5 mg/L NAA與小薊管狀小花以0.2 mg/L BA或0.5 mg/L BA與0.5 mg/L NAA組合處理之MS固體基本培養基照光處理僅獲得少量芽體增殖。而於黑暗處理下之大薊與小薊皆不利於芽體再生,而有利於癒合組織形成。
將大薊與小薊大量增殖之芽體進行發根試驗,於1/2 MS或MS固體基本培養基中添加0.1 mg/L NAA可有效提高芽體的發根率,發根後之瓶苗可直接移植至溫室種植。
在大薊與小薊染色體數觀察方面,發現經組織培養所獲得再生植株之染色體數與野生植株都相同,皆為2n=34。大薊染色體全長變異範圍8.17-4.29 μm,小薊染色體全長變異範圍9.20-4.81 μm。通過核型比較,確定是兩個獨立的物種。

The objectives of this study were to establish a generation system for mass propagation of Cirsium japonicum DC. and C. albescens Kitamura.

Chromosome numbers of in vitro regenerated were also inspected. Three types of explants were used for regeneration and the highest shoot number was obtained in MS basal medium containing with 0.2 mg/L BA and 0.1 mg/L for stem-node and leaf explants of C. japonicum DC. and C. albescens Kitamura under the light. Explants of tubular flowers of C. japonicum DC. cultured in the same basal medium containing with 1.0 mg/L in combination with 0.1 mg/L NAA; or with 4.0 mg/L BA in combination with 0.5 mg/L BA, for had only few shoots (1.1-1.8) regenerated, however C. albescens Kitamura regeneration shoots were found in a medium of 0.2 mg/L BA in combination with 0.5 mg/L NAA and a medium of 0.5mg/L BA in combination with 0.5 mg/L BA. Although a much less or no generated shoots was found in the lightness for all types of explants for both C. japonicum DC. and C. albescens Kitamura, it was found that in the formation of callus was enhanced.

The in vitro regenerated shoots were tested for root formation; highest root formation rates were obtained in a half or full strength of MS medium containing with 0.1 mg/L NAA. The rooted shoots were transplanted directly to the greenhouse.

Chromosome number from root tip cell of in vitro generated plants of C. japonicum DC. and C. albescens Kitamura were inspected and the identical chromosome numbers were found for in vitro and wild plants with the number of 2n=34. The length of chromosomes of C. japonicum DC. was ranged from 8.17-4.29 μm and 9.20-4.81 μm for C. albescens Kitamura. Comparison of the karyotypes form these two species, it is considered that C. japonicum DC. and C. albescens Kitamura are two independent species.
URI: http://hdl.handle.net/11455/36814
其他識別: U0005-2607200609264100
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