Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/36815
標題: 褪黑激素在聖約翰草中含量受週期及光線影響之研究
Study on effects of period and light on melatonin contents in St. John's wort(Hypericum perforatum L.)
作者: 鍾明修
關鍵字: melatonin;褪黑激素;St. John's wort;photoperiod;circadian clock;聖約翰草;光週期;概日時鍾
出版社: 農藝學系所
引用: 李會寧、王存蓮。2000。貫葉連翹的研究進展與可持續發展之對策。 漢中師學院院報 18(1):79-82。 張家綸。2007。鋅化合物對聖約翰草生長及有效成分影響之研究。國立中興大學生命科學系。碩士論文。台中。 黃敏、陳珠、胡劍波、何順志。2000。貴州金絲桃屬藥用植物種類與分布。中國中藥雜誌 25(8):458-461。 梁小燕。 1998。金絲桃屬植物的研究進展。廣西植物 18 (3):256-262。 蔡允真。2009。臺灣地區貫葉連翹種子生產與週年栽培之研究。國立 中興大學農藝系。碩士論文。台中。 臺灣植物誌第二版編輯委員會。1996。臺灣植物誌。pp.694-714。 Afreen, F., S. M. A. Zobayed and T. Kozai. 2006. Melatonin in Glycyrrhiza uralensis: response of plant roots to spectral quality of light and UV-B radiation. J. Pineal Res. 41: 108–115. Agostinis, P., A. Vantieghem, W. Merlevede, and P. A. M. Witte. 2002. Hypericin in canner treatment: more light on the way. Int. J. Biochem. Cell Biol. 34: 221-241. Allegra, M., R.J. Reiter, D.-X. Tan, C. Gentile, L. Tesoriere and M.A. Livrea. 2003. The chemistry of melatonin’s interaction with reactive species. J. Pineal Res. 34: 1–10. Antolın, I. and R. Hardeland. 1997. Suppression of the Gonyaulax glow peak by paraquat and its restoration by melatonin. In: Biological Rhythms and Antioxidative Protection. Hardeland R, ed. Cuvillier, Goettingen pp. 86–97. Arnao, M. B. and J. Hernandez-Ruiz. 2006. The Physiological Function of Melatonin in Plants. Plant Signal. Behav. 1: 89-95. Balzer, I., B. Bartolomaeus and B. Hocker. 1998. Circadian rhythm of melatonin content in chlorophyceae. Abstracts of the Workshop of the European Society for Chronobiology: News From the Plant Chronobiology Research. Bilia, A. R., S. Gallori, and F. F. Vincieri. 2002. St. John`s wort and depression efficacy, safety and tolerability-an update. Life Sci. 70: 3077-3096. Briskin, D. P. 2000. Medicinal plants and phytomedicines-Linking plant biochemistry and physiology to human health. Plant Physiol. 124: 507-514. Carlo, G. D., F. Borrelli, E. Ernst, and A. A. Izzo. 2001. St. John` wort:Prozac from the plant kingdom. Trends Pharmacol. Sci. 22: 292-297. Cassone, V. M. 1990. Effects of melatonin on vertebrate circadian systems. Trends Neurosci 13: 457–464. Dona, M., I. Dell’Aica, E. Pezzato, L. Sartor, F. Calabrese, M. Della Barbera, A. Donella-Deana, G. Appendino, A. Borsarini, R. Caniato and S. Garbisa. 2004. Hyperforin Inhibits Cancer Invasion and Metastasis. Cancer Res. Sep 1; 64(17):6225-32. Dubbels, R., R. J. Reiter, E. Klenke, A. Goebel, E. Schnakenberg, C. Ehlers, H.W. Schiwara and W. Schloot. 1995. Melatonin in edible plants identified by radioimmunoassay and by high performance liquid chromatography-mass spectrometry. J. Pineal Res. 18: 28-31. Guerrero, J. R,. P. Garcia-Ruiz,. J. Sanchez-Bravo,. M. Acosta, and M. B. Arnao1,. 2001. Quantitation of indole-3-acetic acid by LC with electrochemical detection in etiolated hypocotyls of Lupinus albus. J. Liq. Chromatogr. Rel. Tech. 24: 3095-3104. Hardeland, R. and B. Fuhrberg. 1996. Ubiquitous melatonin – presence and effects in unicells, plants and animals. Trends. Comp. Biochem. Physiol. 2: 25-45. Hardeland, R. and B. Poeggeler . 2003. Non-vertebrate melatonin. J. Pineal Res.34: 233–241. Hardeland, R. 2005. Antioxidative protection by melatonin—Multiplicity of mechanisms from radical detoxification to radical avoidance. Endocrine 27: 119–130. Hardeland, R., S.R. Pandi-Peruma and D. P. Cardinali. 2006. Melatonin. Int. J. Biochem. Cell Biol. 38 : 313–316. Hernandez-Ruiz, J., A. Cano and M. B. Arnao. 2004. Melatonin: a growth stimulating compound present in lupin tissues. Planta 220: 140–144. Hernandez-Ruiz, J., A. Cano and M. B. Arnao. 2005. Melatonin acts as a growth stimulating compound in some monocot species. J. Pineal Res. 39: 137-142. Hernandez-Ruiz. 2006. The Physiological Function of Melatonin in Plants. Plant Signal. Behav. 1: 89-95. Kolar, J., C. H. Johnson and I. Machackova1. 2003. Exogenously applied melatonin (N-acetyl-5-methoxytryptamine) affects flowering of the short-day plant Chenopodium rubrum. Physiol. Plant. 118: 605-612. Kolar, J. and I. Machackova. 2005. Melatonin in higher plants: occurrence and possible functions. J. Pineal Res. 39: 333–341. Lerner, A. B., J. D. Chase and Y. Takahashi. 1958. Isolation of melatonin, the pineal factor that lightens mclanocytes. J. Am. Chem. Soc. 80: 2057-2058. Lewy, A. J. 2000. Melatonin as a marker and phase-resetter of circadian rhythms in humans. In: Melatonin After Four Decades: An Assessment of its Potential. Olcese J, ed. Kluwer Academic/Plenum Publishers. New York 425-434. Markgrafenheide, Germany.Brainard, G. C., J. D. Chase, B. A. Richardson and R. J. Reiter. 1982. Pineal melatonin in Syrian hamsters: circadian and seasonal rhythms in animal maintained under laboratory and natural conditions. Neuroendocrinology 39: 342-348. Mayo, J. C., R. M. Sainza, I. Antolin, F. Herreraa,V. Martin and C. Rodriguez. 2002. Melatonin regulation of antioxidant enzyme gene expression. Cell. Mol. Life Sci. 59: 1706–1713. Miller, A. L. 1998. St. John’s wort (Hypericum perforatum): clinical effects on depression and other conditions. Med. Rev. 3: 18-26. Mukherjee, P. K., Saha, K., Murugesan, T., Mandal, S. C., Pal, M. And Saha, B. P. 1998. Screening of anti-diarrhoeal profile of some plant extracts of a specific region of West Bengal, Indin. J. Ethnopharmacol. 60 (1): 85-89. Muller, W. E. 2003. Current St. John`s wort reseach from mode of action to clinical efficacy. Pharmacol. Res. 47: 101-109. Murch, S. J. and P. K. Saxena. 2000. Melatonin:a potential regulator of plant growth and development? In Vitro Cell. Dev. Biol. Plant 38: 531-536. Nathan, P. J. 1999. The experimental and clinical pharmacology of St John`s Wort (Hypericum perforatum). Mol. Pschiatry 4: 333-338. Pal, D. and A. K. Mitra. 2006. MDP-and CYP3A4-mediated drug-herbal interaction. Life Sci. 78: 2131-2145. Pandi-Perumal, S. R., V. Srinivasan, G. J. M. Maestroni, D. P. Cardinali, B. Poeggeler and R. Hardeland. 2006. Melatonin: Nature’s most versatile biological signal? FEBS J. 273: 2813–2838. Poeggeler, B., S. Thuermann, A. Dose, M. Schoenke, S. Burkhardt and Hardeland, R. 2002. Melatonin’s unique radical scavenging properties—Roles of its functional substituents as revealed by a comparison with its structural analogs. J. Pineal Res. 33: 20–30. Posmyk, M. M. and K. M. Janas. 2009. Melatonin in plants. Acta Physiol Plant 31: 1–11. Pothinuch, P. and S. Tongchitpakdee. 2011. Melatonin contents in mulberry (Morus spp.) leaves: Effects of sample preparation, cultivar, leaf age and tea processing. Food Chemistry 128: 415–419. Reiter, R. J. 1991. Pineal melatonin: cell biology of its physiological interactions. Endocr. Rev. 12:151–181. Reiter, R. J. 1993. The melatonin rhythm: both a clock and a calendar. Cell. Mol. Life Sci. 49: 648-653. Reiter, R.J. and D. X. Tan, D. Acuna-Castroviejo, et al. 2000. Melatonin: mechanisms and actions as an antioxidant. Curr. Top. Biophys 24: 171–183. Robson, N. K. B. 1977. Studies in the genus Hypericum perforatum L. (Guttiferae). I. Infrageneric classification. Bull Br. Mus. Nat. Hist. (Botany)5: 293-355. Robson, N. K. B. 1981. Studies in the genus Hypericum perforatum L. (Guttiferae). 2. Characters the genus. Bull Br. Mus. Nat. Hist. (Botany) 8: 55-226. Robson, N. K. B. 2003. Hypericum botany. In Ernst, E, (Ed.), Hypericum; The Genus Hypericum. Taylor and Francis, New York. pp. 1-22. Tan, D. X., L. C. Manchester, R. M. Sainz and R. J. Reiter. 2003. Melatonin: a hormon, a tissue factor, an autocoid, a paracoid and an antioxidant vitamin. J Pineal Res 34:75–7. Van Tassel, D. L., N. Roberts, A. Lewy and S. D. O’Neill. 2001. Melatonin in plant organs. J. Pineal Res. 31: 8–15. Vivien-Roels, B. and P. Pevet. 1993. Melatonin: presence and formation in invertebrates. Experimentia 49: 642-647. Wills, C. J., M. Petersen, W. A. Bryant, M. Reichle, G. J. Saucedo, S. Tan, G. Taylor and J. Treiman. 2000. A siteconditions ap for California based on geology and shear-wave velocity. Bull. Seismol. Soc. Amer. 90: 187-208.
摘要: 
本研究主要以聖約翰草(Hypericum perforatum L.)作為試驗材料,利用高效液相色層分析儀(high performance liquid chromatoghraphy,HPLC)來分析其褪黑激素成分受時間、空間部位與光線影響下之變化。在不同部位的分佈上,結果顯示此藥草中褪黑激素主要出現在花與葉部當中。在5種不同光週期處理下,以18 h/6 h(light/dark)條件下培養的聖約翰草,其生長勢與葉片褪黑激素含量最佳。進一步在不同光質與光強度的試驗中,45 μmole photons m-2 s-1藍光的處理下,聖約翰草葉片中褪黑激素之含量為最高(131 μ g/g F. W.)。此外,紅光處理下,其葉片中褪黑激素的含量也超過白光下的處理。在金絲桃屬植物種間的差異分析,顯示受測的多種金絲桃屬植物中均可偵測到褪黑激素的存在,除了聖約翰草的含量較高外,H. coris L. 與 H. kouytchense Lev. 此兩種金絲桃屬植物,其葉片中褪黑激素的含量皆超出聖約翰草甚多,是相當特別且值得後續進一步深入的探討。在時間的調節上,聖約翰草葉片中褪黑激素含量的變化,在同步化有時間賦予者和持續光照恆常的條件下,褪黑激素的含量皆呈現~ 24 h週期性的韻律變化,清楚的顯示它是受到概日時鐘(circadian clock)之調節。進一步研究不同濃度的褪黑激素、生長素或色胺酸對聖約翰草種子發芽及萌芽種子生長的影響上,顯示聖約翰草種子發芽率以及胚根和胚軸的生長受褪黑激素的調節,且隨著濃度增加而有抑制的現象。

Hypericum perforatum L. (St. John's wort) has been taken as the main experimental material in this study . Melatonin contents of St. John's wort under temporal, spatial and light regulation have been investigated and analyzed by high performance liquid chromatography (HPLC). For spatial regulation, it exhibits that melatonin occurs mainly in the flower and leaf parts of this medicinal herb. The growth and melatonin content of St. John's wort are optimal under 18 h/6 h (light/dark) cycle among five different photoperiod treatments. For further experiments with light quality and intensity, it shows that melatonin content in the leaves of St. John's wort is highest under the treatment of blue light with intensity about 45 μ mole photons m-2 s-1. In addition, melatonin content in the leaves of St. John's wort under red light treatment also exceeds the levels under two white light treatments. For interspecific comparison of Hypercum spp., it exhibits that elatonin is detectable among many species tested. Beside H. perforatum L. with higher level, melatonin contents in the leaves of H. coris L. and H. kouytchense Lev. all exceed much more than the level of St. John's wort. It is quite special and worth being explored further. For temporal regulation, the variation of melatonin contents in leaves of H. perforatum L. exhibits a rhythmic periodicity of ~24 h both under entrained (with Zeitgeber) and constant light conditions. It shows clearly that the rhythm is under control of circadian clock. For the influence of melatonin, IAA and tryptophan with different concentrations on the seed germination and growth of germinating seeds of St. John's wort, it has shown that the germination percentage, radical and hypocotyl growth can be regulated by melatonin and be inhibited in proportion to the increase of its concentration.
URI: http://hdl.handle.net/11455/36815
其他識別: U0005-2208201117210700
Appears in Collections:農藝學系

Show full item record
 

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.