Please use this identifier to cite or link to this item:
標題: 玉米B染色體異染色質區基因組片段之特性分析
Characterization of genomic fragments from heterochromatic regions on maize B chromosome
作者: 黃珮瑜
Huang, Pei-Yu
關鍵字: B chromosome;B染色體;heterochromatic regions;CL-repeat;fluorescence in situ hybridization (FISH);phylogenetic analysis;異染色質區;CL-repeat;螢光原位雜合;系統演化分析
出版社: 農藝學系所
引用: Alfenito, M. R. and J. A. Birchler. 1993. Molecular characterization of a maize B chromosome centric sequence. Genetics 135: 589-597. Ananiev, E. V., R. L Phillips, and H. W. Rines. 1998. Chromosome- specific molecular organization of maize (Zea mays L.) centromeric regions. Proc. Natl. Acad. Sci. USA 95: 13073-13078. Auger, D. L. and J. A. Birchler. 2002. Maize tertiary trisomic stocks derived from B-A translocations. J. Hered. 93: 42-47. Beckett, J. B. 1978. B-A translocation in maize. J. Hered. 69: 27-36. Beckett, J. B. 1991. Cytogenetics, genetic and plant breeding applications of B-A translocation in maize. pp. 493-529. in Chromosome engineering in plants: Genetics, Breeding, Evolution. Vol. IIA, edited by P. K. Gupta and T. Tsuchiya. Elsevier, Amsterdam. Beckett, J. B. 1994. Locating recessive genes to chromosome arm with B-A translocations. pp. 315-327. in The Maize Handbook. edited by M. Freeling and V. Walbot. Springer-Verlag, New York. Birchler, J. A. 1994. A study of enzyme activities in a dosage series of the long arm of chromosome one in maize. Genetics 92: 1211-1229. Birchler, J. A. 1994. Dosage analysis using B-A translocations. pp. 328-329. in The Maize Handbook. edited by M. Freeling and V. Walbot. Springer-Verlag, New York. Carlson, W. R. and T. S. Chou. 1981. B chromosome nondisjunction in corn: Control by factors near the centromere. Genetics 97: 379-389. Carlson, W. 2009. pp. 459-480. The B chromosome of maize. in Handbook of Maize. edited by J. L. Bennetzen and S. Hake. Springer-Verlag, New York. Cheng, Y. M. and B. Y. Lin. 2003. Cloning and characterization of maize B chromosome sequences derived from microdissection. Genetics 164: 299-310. Cheng, Y. M. and B. Y. Lin. 2004. Molecular organization of large fragments in the maize B chromosome: indication of a novel repeat. Genetics 166: 1947-1961. Cheng, Y. M. 2010. Evolution of the heterochromatic regions on maize B long arm based on the sequence structure of CL-repeat variants. Chromosome Res. 18: 605-619. Chilton, M. D. and B. J. McCarthy. 1973. DNA from maize with and without B chromosomes: a comparative study. Genetics 74: 605-614. González-Sánchez, M., E. González-González, F. Molina, A. M. Chiavarino, M. Rosato and M. J. Puertas. 2003. One gene determines maize B chromosome accumulation by preferential fertilisation; another gene(s) determines their meiotic loss. Heredity 90: 122-129. Jin, W. W., J. C. Lamb, J. M. Vega, R. K. Dawe, J. A. Birchler and J. Jiang. 2005. Molecular and functional dissection of the maize B chromosome centromere. Plant Cell 17: 1412-1423. Jin, W. W., J. R. Melo, K. Nagaki, P. B. Talbert, S. Henikoff, R. K. Dawe and J. Jiang. 2004. Maize centromeres: organization and functional adaptation in the genetic background of oat. Plant cell 16: 571-581. Jones, R. N. 1995. Tansley review no. 85: B chromosome in plant. New Phytol. 131: 411-434. Jones, N. and A. Houben. 2003. B chromosome in plants: escapees from the A chromosome genome? Trends Plant Sci. 8: 417-423. Jones, R. N., W. Viegas and R. Houben. 2007. A century of B chromosomes in plants: So what? Annals of Botany 167: 1-9. Kaszás, É. and J. A. Birchler. 1996. Misdivision analysis of centromere structure in maize. EMBO J. 15: 5246-5255. Kaszás, É. and J. A. Birchler. 1998. Meiotic transmission rates correlate with physical features of rearranged centromeres in maize. Genetics 150: 1683-1692. Kato, A., J. C. Lamb and J. A. Birchler. 2004. Chromosome painting in maize using repetitive DNA sequences as probes for somatic chromosome identification. Proc. Natl. Acad. Sci. USA 101: 13554-13559. Kuwada, Y. 1915. Ueber die chromosomeenzahl von Zea mays L. Bot. Mag. Tokyo 29: 83-89. Lamb, J. C., A. Kato and J. A. Birchler. 2005. Sequences associated with A chromosome centromeres are present throughout the maize B chromosome. Chromosoma 113: 337-349. Lamb, J. C., N. C. Riddle, Y. M. Cheng, J. Theuri and J. A. Brichler. 2007. Localization and transcription of a retrotransposon-derived element on the maize B chromosome. Chromosome Res. 15: 383-398. Lin, B. Y. 1978. Regional control of nondisjunction of the B chromosome in maize. Genetics 90: 613-627. Lin, B. Y. 1979. Two new B-10 translocations involved in the control of nondisjunction of the B chromosome in maize. Genetics 92: 931-945. Lin, B. Y. and H. P. Chou. 1997. Physical mapping of four RAPDs in the B chromosome of maize. Theor. Appl. Genet. 94: 534-538. Lo, K. L., Y. P. Lin, L. J. Chen and B. Y. Lin. 2009a. Isolation and characterization of new maize B sequences from a microdissected library. Plant Mol. Biol. Rep. 27: 350-354. Lo, K. L., S. F. Peng, L. J. Chen and B. Y. Lin. 2009b. Tandem organization of StarkB element (22.8 kb) in the maize B chromosome. Mol. Genet. Genomics 282: 131-139. Longley, A. E. 1927. Supernumerary chromosomes in Zea mays. J. Agric. Res. 35: 769-784. McMillan, D. E., D. G. Roupkias and J. G. Scandalios. 1979. Chromosomal location of two mitochondrial malate dehydrogenase structural genes in Zea mays using trisomics and B-A translocations. Genetics 92: 1241-1250. Nagaki, K., J. Song, R. M. Stupar, A. S. Parokonny, Q. Yuan, S. Quyang, J. Liu, J. Hsiao, K. M. Jones, R. K. Dawe, C. R. Buell and J. Jiang. 2003. Molecular and cytological analyses of large tracks of centromeric DNA reveal the structure and evolutionary dynamics of maize centromeres. Genetics 163: 759-770. Peng, S. F., Y. P. Lin and B. Y. Lin. 2005. Characterization of AFLP sequences from regions of maize B chromosome defined by 12 B-10 L translocations. Genetics 169: 375-388. Peng, S. F. and Y. M. Cheng. 2011. Characterization of satellite CentC repeats from heterochromatic regions on the long arm of maize B-chromosome. Chromosome Res. 19: 183-191. Randolph, L. F. 1928. Types of supernumerary chromosomes in maize. Anat. Rec. 41: 102. Rusche, M. L., H. L. Mogensen, L. Shi, P. Keim, M. Rougier, A.Chaboudf and C. Dumas. 1997. B chromosome behavior in maize pollen as determined by a molecular probe. Genetics 147: 1915-1921. Rusche, M. L., H. L. Mogensen, A. Chaboud , J. E. Faure, M. Rougier, P. Keim and C. Dumas. 2001. B chromosomes of maize (Zea mays L.) are positioned nonrandomly within sperm nuclei. Sex. Plant Reprod. 13: 231-234. Stark, E. A., L. Connerton, S. T. Bennett, S. R. Barnes, J. S. Parker and J. W. Forster. 1996. Molecular analysis of the maize B-chromosome. Chromosome Res. 4: 15-23. Weber, D. and T. Helentjaris. 1989. Mapping RFLP loci in maize using B-A translocations. Genetics 121: 583-590. Wilson, E. B. 1907. Note on the chromosome groups of Metapodius and Banasa. Biological Bulletin XII: 303-313.
本研究利用限制酵素剪切、南方氏雜合及螢光原位雜合對34個玉米B染色體異染色質區的基因組片段進行特性分析。利用NotI酵素剪切估計基因組片段大小介於11 kb~20 kb之間,依據XbaI片段數量與大小,剔除6個重複的基因組片段;以B染色體異染色質區專一性序列CL-repeat進行南方氏雜合分析,確認基因組片段源自B染色體異染色質區,藉由CentC的南方氏雜合分析,顯示3個基因組片段同時帶有CL-repeat與CentC序列;接著以各基因組片段作為螢光原位雜合探針,分析各片段在染色體之分布,在有絲分裂中期染色體可觀察到6種訊號分布類型;而在減數分裂粗絲期B染色體訊號,由僅在4個異染色質區至整個B染色體的分布皆有。最後,利用CL-repeat專一性引子增幅各基因組片段內的CL-repeat,可增幅出1.6-kb、0.75-kb、0.65-kb以及0.15-kb四種產物,進一步將所有產物選殖並定序,再利用已經定位的28個1.6-kb CL-repeat對本研究的21個1.6-kb CL-repeat進行系統演化分析,藉此可以將各CL-repeat及其所在的基因組片段定位在B染色體特定異染色質區。此結果使我們對玉米B染色體異染色質區有更多的了解。

In the present study, restriction enzyme digestion, Southern hybridization and fluorescence in situ hybridization (FISH) were used to characterize 34 genomic fragments from heterochromatic regions on maize B chromosome. By using NotI digestion, the size of genomic fragments were estimated to be between 11 kb to 20 kb. According to the number and size of XbaI fragments, six duplication genomic fragments were eliminated. The heterochromatic origins of each fragment were verified by Southern hybridization with the B heterochromatin specific CL-repeat. Three genomic fragments containing both CL-repeat and CentC sequences were revealed by Southern hybridization with CentC as probe. Subsequently, FISH analysis with each fragment as probe was applied to detect their distributions on chromosome. Six types of signal distributions were observed on mitotic chromosomes. The signal distributions on the pachytene B chromosome varied from signals on the four heterochromatin region to on the entire B chromosome. Finally, four CL-repeat products, 1.6-kb, 0.75-kb, 0.65-kb and 0.15-kb, amplified from those genomic fragments by a pair of CL-repeat specific primers were cloned for sequences analysis. The resulting 21 1.6-kb CL-repeat were calculated with 28 previously mapped CL-repeat by phylogenetic analysis to map each CL-repeat and their original genomic fragments onto define heterochromatic region of B chromosome. The results can help to get more understanding of the heterochromatic regions on maize B chromosome.
其他識別: U0005-2907201113182200
Appears in Collections:農藝學系

Show full item record
TAIR Related Article

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.