Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/36842
標題: 利用簡單重複序列分子標誌定位矮生水稻Shiokari-d6基因座
Using rice SSR markers to map the dwarf gene of Shioakri-d6 locus
作者: 黃冠富
Hung, Kung-Fu
關鍵字: d6 dwarf gene;d6矮生水稻;near-isogenic lines;simple sequence repeat markers;quantitative trait locus;map-based cloning;近同源系;簡單序列重複分子標誌;數量性狀基因座;據圖選殖
出版社: 農藝學系所
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摘要: 
水稻是世界上極為重要的作物,全球有一半以上人口倚賴水稻為主食。而株高是水稻非常重要的農藝性狀,近代育種學者將半矮性基因導入水稻中,使植株矮化且能耐高氮肥與抵抗強風造成的倒伏,因此水稻收穫指數提高、產量大增,造就水稻綠色革命。日本北海道大學在田間發現的 d6 矮生水稻,其株高矮小,且節間自第二節以下全部縮短,此為 d6 型的矮生水稻。
近同源系( near isogenic lines, NILs )是精確推測數量性狀基因座( quantitative trait locis, QTLs )以及執行據圖選殖( map-based cloning )優良的材料。本試驗利用日本栽培種水稻 Shiokari 與其矮生近同源系 Shioakri-d6 所建構的 NIL- F2 族群共168株,使用227個簡單序列重複( simple sequence repeat )分子標誌針對全基因組進行篩選,並利用 Qgene 統計軟體以單點定位法分析具有多型性之分子標誌。結果在第七號染色體上臂發現七個與株高緊密連鎖之分子標誌,其中以 KU-d6-4 和 KU-d6-1 與株高相關性最高。而以 Qgene 利用區間定位法( interval mapping )分析,進一步將 d6 基因鎖定於 KU-d6-3 與 KU-d6-4 區間。

Rice is one of the most important crops in the world, over half population relies on it as the staple food in the world wide. Plant height is very important agricultural characteristic in rice production. The semi-dwarf gene was in trogressed into the rice in recent the half century, which resisted the lodging under high nitrogen and high wind land. Thus, the introgressed semi-dwarf genes provided higher harvest index and yield which “Green Revolution” was called. One of dwarf mutants of rice, d6 , had been identified and characterized by the scholar of Japan Hokkaido university. In the d6-type mutant, the internodes below the uppermost are shortened.
Near-isogenic lines ( NILs ) are ideal materials for preise estimation the efferts of quantitative trait loci ( QTL ) and map-based cloning of target genes. In this study, we analyzed the genetic bases of plant height, using a population of 168 F2 derived from a cross between Japan local cultivar Shiokari and NIL Shiokari-d6 ( NIL- F2 ). By testing 168 NIL- F2 with 227 even distributed simple sequence repeats ( SSRs ) markers, we identified the markers that were tightly linked to d6 locus on chromosome 7. The two markers KU-d6-1 and KU-d6-4 had the highest R2 score by single-point analysis. In addition, using interval mapping, the d6 gene locus was mapped in the interval between KU-d6-3 and KU-d6-4.
URI: http://hdl.handle.net/11455/36842
其他識別: U0005-2008200716310900
Appears in Collections:農藝學系

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