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dc.contributorXin-De Shihen_US
dc.contributorJun-Yi Wuen_US
dc.contributorYet-Pole Ien_US
dc.contributorHong-Wei Yenen_US
dc.contributor.advisorYung-Chuan Liuen_US
dc.contributor.authorChen, Yu-Juien_US
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dc.description.abstract本研究使用具有抗真菌活性的鏈黴菌Streptomyces padanus PMS-702來進行多烯類大環內酯抗生素Fungichromin(治黴色基素)之醱酵生產研究。此抗生素不但存在生物防治潛力(例:對立枯絲核菌及番茄晚疫病有良好抑制效果),還可應用於如腫瘤之臨床醫療上。 本研究主要是於液態培養中,透過葡萄糖、油脂消耗、菌重、Fungichromin產物的時間關係圖,了解油脂添加時機與不同油脂對Fungichromin產物及菌的變化。實驗結果顯示不飽和脂肪酸可提升Fungichromin之產量。利用顯微鏡觀察菌體及菌球內Fungichromin晶體之型態,添加油脂的菌Fungichromin晶體分佈在發酵中密度越高,Fungichromin產量越大。 在油脂添加方面,玉米油的添加有助於菌體乾重的提升,而橄欖油的添加對於Fungichromin產量有提升效果。在培養基中添加2 %界面劑Span80並於第二天再添加1%玉米油,結果顯示,醱酵至第4天時,Fungichromin產量可達約1700 mg/L,約為未添加時(Fungichromin產量16.7 mg/L)的8.5倍。將此操作於氣舉式醱酵槽中進行。在溫度23.8 ℃、通氣量2.5 vvm、不控制pH值的條件下,於培養第7天Fungichromin產量可達到2196 mg/L的產量,約為不添加時的9.34倍。 關鍵字:鏈黴菌、多烯類大環內酯、治黴色基素、生物防治、氣舉式發酵槽zh_TW
dc.description.abstractStreptomyces padanus PMS-702 not only has the potential for preventing and curing the organism attack (for example, it can control the diseases of Rhizoctonia damping-off of cabbage and tomoto late blight), but also can be applied to clinical medical treatment of tumor. This research is focused on applying the air-lift bioreactor to cultivate Streptomyces padanus PMS-702 to produce an anti-fungus antibiotic, fungichromin. Several factors including glucose concentration, oil types and concentrations would affect microbial growth and fungichromin production in the submerged culture. Different oils combination and addition was also shown to be a critical factor influencing the production. Further study exhibited that the addition of 2% Span80 at day 0 along with 1% corn oil at day 2could give a high fungichromin production of 1700mg/L. Generally, the addition of corn oil was more in favor to microbial growth and olive oil supplementation was more beneficial to fungichromin production. A 5L air-lift bioreactor was applied to cultivate Streptomyces padanus PMS-702 under the following conditions: 23.8 ℃, 2.5 vvm, without pH control, 2% Span80 at day 0 along with 1% corn oil at day 2 under this condition, the production of fungichromin would 2190 mg/L, would reach a 9.34-fold increase compared to that with basal medium. Keywords : Streptomyces padanus, fungichromin, biological control, polyene macrolides, air-lift bioreactoren_US
dc.description.tableofcontents中文摘要 I Abstract II 目次 III 表次目 VI 圖次目 VII 一、緒論 1 二、文獻回顧 3 (一)、 抗生素 3 1. 前言 3 2. 抗生素分類與作用機制 3 3. 抗生素應用 5 4. 抗生素產生方式 5 5. 抗生素製程與一般醱酵製程之比較 7 6. 抗生素生產所遇到的問題 8 7. 提高抗生素產量的方法 11 (二)、 鏈黴菌 13 1. 放線菌之簡介 13 2. 鏈黴菌之分類及特性 14 3. 鏈黴菌之生物病害防治 15 (三)、 Fungichromin 17 1. Fungichromin之介紹 17 2. Fungichromin之結構與性質 17 3. Fungichromin之分離鑑定及農業上應用 22 4. 發酵生產Fungichromin之製程放大 22 三、材料與方法 24 (一)、 菌種 24 1. 菌種來源 24 2. 菌種保存 24 (二)、 培養基組成 24 1. 前培養培養基 24 2. 主培養培養基 24 (三)、 搖瓶添加物實驗 26 1. 含玉米油培養基添加不同油脂 26 2. 含玉米油培養基添加界面活性劑 26 (四)、 醱酵槽批次醱酵條件設定實驗 26 1. 五公升酵槽操作程序 26 2. 不同通氣量對Streptomyces padanus PMS-702於醱酵槽批次醱酵影響 27 3. 內管影響對Streptomyces padanus PMS-702於醱酵槽批次醱酵之機制 28 4. 最佳醱酵條件於氣舉式醱酵槽中應用探討 28 (五)、 不同萃取時間方式觀察Fungichromin產量 30 (六)、 Streptomyces padanus PMS-702之生長型態觀察 30 (七)、 分析方法 30 1. 菌體乾重測定 30 2. 油脂的殘餘量測定 30 3. 殘糖量分析 30 4. Fungichromin定量分析 34 四、結果與討論 36 (一)、 氣舉式醱酵槽批次液態醱酵 36 1. 不同通氣量對Streptomyces padanus PMS-702於醱酵槽中批次醱酵之影響 36 2. 不同內管對Streptomyces padanus PMS-702於氣舉式批次醱酵之影響 40 (二)、 搖瓶試驗 45 1. 萃取方式改進 45 2. 探討玉米油及葡萄糖之消耗對Streptomyces padanus PMS-702之菌乾重與Fungichromin之影響 47 3. 探討不同添加物對Streptomyces padanus PMS-702於搖瓶醱酵之影響 50 4. 油脂混合添加劑對Streptomyces padanus PMS-702之影響 55 5. 界面活性劑與油脂之混合添加劑對Streptomyces padanus PMS-702之影響 61 6. 混合添加劑對Streptomyces padanus PMS-702影響之綜合及整理比較 65 7. 探討不同添加天數對Fungichromin產量之影響 68 (三)、 最佳醱酵條件於氣舉式醱酵槽中應用探討 72 (四)、 Streptomyces padanus PMS-702的菌體型態 74 五、結論與展望 77 (一)、 結論 77 (二)、 未來與展望 77 參考文獻 78zh_TW
dc.subjectStreptomyces padanusen_US
dc.subjectbiological controlen_US
dc.subjectpolyene macrolidesen_US
dc.subjectair-lift bioreactoren_US
dc.title應用氣舉式發酵槽於Streptomyces padanus PMS-702 生產Fungichromin之研究zh_TW
dc.titleProduction of Fungichromin by Streptomyces padanus PMS-702 in Air-lift bioreactoren_US
dc.typeThesis and Dissertationzh_TW
item.openairetypeThesis and Dissertation-
item.fulltextno fulltext-
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