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標題: 樟芝抗發炎活性成分及其研究
Anti-inflammatory Constituent from Antrodia cinnamomea and Its Mechanisms
作者: 王雅欣
關鍵字: Antrodia cinnamomea;樟芝;Anti-inflammation;Antrocamphin A;NF-κB;抗發炎活性;Antrocamphin A;NF-κB
出版社: 農藝學系所
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Active extracts of wild fruiting bodies of Antrodia camphorate (EEAC) induce leukemia HL 60 cells apoptosis partially through histone hypoacetylation and synergistically promote anticancer eVect of trichostatin A. Arch. Toxicol. Rao, Y. K., S. H. Fang and Y. M. Tzeng. 2007. Evaluation of the anti-inflammatory and anti-proliferation tumoral cells activities of Antrodia camphorata, Cordyceps sinensis, and Cinnamomum osmophloeum bark extracts. J. Ethnopharmacol. 114: 78–85. Shen, C. C., Y. C. Kuo, R. L. Huang, L. C. Lin, M. J. Don, T. T. Chang and C. J. Chou. 2003. New ergostane and lanostane from Antrodia camphorata. Int. J. Chin. Med. 14: 247-258. Shen, Y. C., C. J. Chou, Y. H. Wang, C. F. Chen, Y. C. Chou and M. K. Lu. 2004. Anti-inflammatory activity of the extracts from mycelia of Antrodia camphorata cultured with water-soluble fractions from five different Cinnamomum species. FEMS micro. biol. Lett. 231: 137-143. Song, T. Y. S. L. Hsu, C. T. Yeh and G. C. Yen. 2005a. 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樟芝(Antrodia cinnamomea)為台灣知名的傳統藥用真菌,民間長久認為其對食物中毒、腹瀉、嘔吐和農藥中毒等具有解毒作用。本研究首先以脂多醣(lypopolysaccharides)誘導小鼠急性發炎的動物模式,評估樟芝子實體抽出物之抗發炎活性,試驗結果發現樟芝乙醇抽出物具有顯著的抗發炎活性,可有效抑制小鼠體內因LPS所誘導的一氧化氮生成酵素、第二型環氧酵素和核轉錄因子-κB等酵素之表現。接著並以活性為導向的分離策略,從樟芝子實體乙醇抽出物中分離出具抗發炎活性成分antrocamphin A,續利用LPS誘導小鼠巨噬細胞(RAW 264.7)產生發炎的模式,解析antrocamphin A之抗發炎機制,結果證實antrocamphin A確可有效抑制一氧化氮自由基和前列腺素的生成,透過蛋白質表現分析得知,antrocamphin A之抗發炎活性是經由抑制一氧化氮生成酵素和第二型環氧酵素的mRNA表現,進而抑制了一氧化氮生成酵素、第二型環氧酵素和核轉錄因子-κB等酵素之表現。本研究為首次以動物模型證實樟芝抽出物可抑制動物體內之發炎反應,並分離獲得其抗發炎活性成分與釐清其作用機制,對於未來進一步的利用具有相當的參考價值。

Antrodia cinnamomea is a well-known traditional medicinal fungus in Taiwan. It has been used for centuries as detoxificant for food poisoning, diarrhea, vomiting and agrochemical poisoning. In the present study, the LPS-challenged ICR mice acute inflammation model was used to evaluate the anti-inflammatory activity of A. cinnamomea. The ethanol extract of A. cinnamomea was proved to have a significant inhibition on iNOS, COX-2, and NF-κB expression in the LPS-induced acute inflammatory mice. To understand the anti-inflammatory mechanism and active compounds of A. cinnamomea, antrocamphin A was purified from previous ethanol extraction using bioactivity-guided fractionation. As results, the antrocamphin A could significantly inhibit NO and PGE2 autacoids production in LPS-induced RAW 264.7 macrophage cells. Meanwhile, the mRNA and protein expression levels of iNOS and COX-2 were inhibited by antrocamphin A in a dose-dependent manner. Antrocamphin A also reduced the translocation of NF-κB induced by LPS, which was associated with the prevention of the degradation of I-κB, and subsequently decreased p65/p50 proteins level in the nucleus. This is the first report demostrating the A. cinnamomea could significant reduce the inflammation in animal. Moreover, the mechanism of its anti-inflammatory compound, antrocamphin A, was also revealed. which could be a valuable reference for developing the pharmaceutical or functional food of A. cinnamomea in the future.
其他識別: U0005-0902200910485500
Appears in Collections:農藝學系

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