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http://hdl.handle.net/11455/36884
標題: | 樟芝抗發炎活性成分及其研究 Anti-inflammatory Constituent from Antrodia cinnamomea and Its Mechanisms |
作者: | 王雅欣 WANG, YA-SIN |
關鍵字: | Antrodia cinnamomea;樟芝;Anti-inflammation;Antrocamphin A;NF-κB;抗發炎活性;Antrocamphin A;NF-κB | 出版社: | 農藝學系所 | 引用: | 鄧富元、龔素芳、夏毅然、高文斌、謝耀東。2006。NF-κB在發炎反應、細胞凋亡與癌症生成過程中的調控。中華牙誌 25: 12-24。 胡鷗、連張飛、張君逸、盧喜。2006。樟芝的藥用保健價值及開發應用。亞熱帶植物科學 35: 75-78。 賴建興。2006。香椿葉萃取物之降低尿酸、抗氧化活性以及安全性讀理評估。國立中興大學農藝學系碩士學位論文。1-73頁。 Ao, Z. H., Z. H. Xu, Z. M. Lu, H. Y. Xu, X. M. Zhang, W. F. Dou. 2009. Niuchangchih (Antrodia camphorata) and its potential in treating liver diseases. J. Ethnopharmacol. 121: 194–212. Chang, J. M., Y. R. Lee, L. M. Hung, S. Y. Liu, M. T. Kuo, W. C. Wen, P. Chen. An extract of antrodia camphorata mycelia attenuates the progression of nephritis in systemic lupus erythematosus–Prone NZB/W F1 Mice. eCAM. 1-7. Chen, C. H. and S. W. Yang. 1995. New steroid acids from Antrodia cinnamomea, a fungal parasite of Cinnamomum micranthum. J. Nat. Prod. 58: 1655-1661. Chen, J. J., W. J. Lin, C. H. Liao and P. C. Shieh. 2007. Anti-inflammatory benzoids from Antrodia camphorata. J. Nat. Prod. 70: 989-992. 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Anti-inflammatory potential of Antrodia camphorate through inhibition of iNOS, COX-2 and cytokines via the NF-kB pathway. Int. Immunopharmacol. 5: 1914–1925. Hseu, Y. C., W. C. Chang, Y. T. Hseu, C. Y. Lee, Y. J. Yech, P. C. Chen, J. Y. Chen and H. L. Yang. 2002. Protection of oxidative damage by aqueous extract from Antrodia camphorata mycelia in normal human erythrocytes. Life Sci. 71: 469- 482. Hsu, Y. L., Y. C. Kuo, P. L. Kuo, L. T. Ng, Y. H. Kuo and C. C. Lin. 2005. Apoptotic effects of extract from Antrodia camphorata fruiting bodies in human hepatocellular carcinoma cell lines. Cancer Lett. 221: 77-89. Hsu,Y. L., P. L. Kuo, C. Y. Cho, W. C. Ni, T. F. Tzeng, L. T. Ng, Y. H. Kuo, C. C. Lin. 2007. Antrodia cinnamomea fruiting bodies extract suppresses the invasive potential of human liver cancer cell line PLC/PRF/5 through inhibition of nuclear factor κB pathway. Food Chem. Toxicol. 45: 1249–1257. Lee, I. H., R. L. Huang, C. T. Chen, H. C. Chen, W. C. Hsu and M. K. Lu. 2002. 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Lu, Y. C. Lai, C. C. Chen, T. H. Hseu, C. T. Kuo and Y. C. Hseu. 2005. Growth inhibition and induction of apoptosis in MCF-7 breast cancer cells by Antrodia camphorata. Cancer Lett. 231:215-227. Yang, S. W., Y. C. Shen and C. H. Chen. 1996. Steroids and triterpenoids of Antrodia cinnamomea – fungus parasitic on Cinnamomum micranthum. Phytochemistry 41: 1389-1392. | 摘要: | 樟芝(Antrodia cinnamomea)為台灣知名的傳統藥用真菌,民間長久認為其對食物中毒、腹瀉、嘔吐和農藥中毒等具有解毒作用。本研究首先以脂多醣(lypopolysaccharides)誘導小鼠急性發炎的動物模式,評估樟芝子實體抽出物之抗發炎活性,試驗結果發現樟芝乙醇抽出物具有顯著的抗發炎活性,可有效抑制小鼠體內因LPS所誘導的一氧化氮生成酵素、第二型環氧酵素和核轉錄因子-κB等酵素之表現。接著並以活性為導向的分離策略,從樟芝子實體乙醇抽出物中分離出具抗發炎活性成分antrocamphin A,續利用LPS誘導小鼠巨噬細胞(RAW 264.7)產生發炎的模式,解析antrocamphin A之抗發炎機制,結果證實antrocamphin A確可有效抑制一氧化氮自由基和前列腺素的生成,透過蛋白質表現分析得知,antrocamphin A之抗發炎活性是經由抑制一氧化氮生成酵素和第二型環氧酵素的mRNA表現,進而抑制了一氧化氮生成酵素、第二型環氧酵素和核轉錄因子-κB等酵素之表現。本研究為首次以動物模型證實樟芝抽出物可抑制動物體內之發炎反應,並分離獲得其抗發炎活性成分與釐清其作用機制,對於未來進一步的利用具有相當的參考價值。 Antrodia cinnamomea is a well-known traditional medicinal fungus in Taiwan. It has been used for centuries as detoxificant for food poisoning, diarrhea, vomiting and agrochemical poisoning. In the present study, the LPS-challenged ICR mice acute inflammation model was used to evaluate the anti-inflammatory activity of A. cinnamomea. The ethanol extract of A. cinnamomea was proved to have a significant inhibition on iNOS, COX-2, and NF-κB expression in the LPS-induced acute inflammatory mice. To understand the anti-inflammatory mechanism and active compounds of A. cinnamomea, antrocamphin A was purified from previous ethanol extraction using bioactivity-guided fractionation. As results, the antrocamphin A could significantly inhibit NO and PGE2 autacoids production in LPS-induced RAW 264.7 macrophage cells. Meanwhile, the mRNA and protein expression levels of iNOS and COX-2 were inhibited by antrocamphin A in a dose-dependent manner. Antrocamphin A also reduced the translocation of NF-κB induced by LPS, which was associated with the prevention of the degradation of I-κB, and subsequently decreased p65/p50 proteins level in the nucleus. This is the first report demostrating the A. cinnamomea could significant reduce the inflammation in animal. Moreover, the mechanism of its anti-inflammatory compound, antrocamphin A, was also revealed. which could be a valuable reference for developing the pharmaceutical or functional food of A. cinnamomea in the future. |
URI: | http://hdl.handle.net/11455/36884 | 其他識別: | U0005-0902200910485500 |
Appears in Collections: | 農藝學系 |
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