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Effects of Plant growth regulators and light conditions on tissue culture of Polygonatum altelobatum Hay.
Long, Pham Phu
|關鍵字:||黃精;Polygonatum altelobatum Hay;組織培養;光條件;tissue culture;light condition;phosphinothricin||出版社:||農藝學系所||引用:||Al-Sslem, M. M. and N. S. Karam. 2001. Auxin, wouding, and propagation medium affect rooting response of stem cutting of Arbutus andrachne. Hort. Sci. 36(5): 976-978. An, J., J. Z. Liu, C. F. Wu, J. Li, L. Dai, E. Damme, J. Balzarini, E. Clercq,F. Chen and J. K. Bao. 2006. Anti-HIV I/II activity and molecular cloning of a novel mannose/sialic acid-binding lectin from rhizome of Polygonatum cyrtonema Hua. Acta Biochimica et Biophysica Sinica,38 (2): 70-78. Arora, R., and S. S. Bhojwani. 1989. In vitro propagation and low temperature storage of Saussurea lappa C. B. Clarke: an endangered medicinal plant. Plant Cell Rep. 8: 44-47. Barbara, N., K. Miczynski, and L. Hudy. 2004. Sugar uptake and utilisation during adventitious bud differentiation on in vitro leaf explants of‘Wegierka Zwykla' plum (Prunus domestica). Plant Cell Tissue Organ Cult. 76: 255-260. Barcelo-Munoz, A., C. L. Encina, E. Simon-Perez, and F. Pliego-Alfaro.1999. Micropropagation of adult avocado. Plant Cell Tissue Organ Cult.58: 11-17. Barciszewski, J., G. S. Siboska, I. S. Ranttan, and B. F. C. Clark. 2000.Occurrence, biosynthesis and properties of kinetin (N6-Furfuryladenine). Plant Growth Regul. 32: 257-265.-65- Berthon, J. Y., S. Ben Tahar, T. Gaspar, and N. Boyer. 1990. Rooting phases of shoots of Sequoiadendron gigantum in vitro and their requirements. Plant Physiol. Biochem. 28: 631-638. Bhagyalakshmi, N. and N. S. Singh. 1988. Meristem culture and micropropagation of a variety of ginger (Zingiber officinale Rosc.) with a high yeild of Oleoresin. J. Hort. Sci. 63 (2): 321-327. Buban, T. 2000. The use of benzyladenine in orchard fruit growing. Plant Growth Regul. 32: 381-390. Carvalho, L. C., M. L. Osorio, M. M. Chaves, and S. Amancio. 2001. Chlorophyll fluorescence as an indicator of photosynthetic functioning of in vitro grapevine and chestnut plantlets under ex vitro acclimatization. Plant Cell Tissue Organ Cult. 67: 271-280. Chand, S., A. K. Sahrawat and D. V. S. S. R. Prakash. 1997. In vitro culture of Pimpinella anisum L. (anise). J. Plant Biochem. Biotech. 6:67-71. Chow, W. S., A. Melis and J. M. Anderson. 1990. Adjustments of photosystem stoichiometry in chloroplasts improved the quantum efficiency of photosynthesis. Proc. Natl. Acad. Sci. 87: 7502-7506. Cristina, M., F. Dos-Santos, M. A. Equibel and V. P. Dos-Santos. 1990. In vitro propagation of the alkaloid producing plant Datura insignis Barb. Rodr. Plant Cell Tissue Organ Cult. 21: 75-78.-66- Cui, K. R., G. M. Xing, X. M. Liu and Y. F. Wang. 1999. Effect of hydrogen peroxide on somatic embryogenesis of Lysium barbarum L. Plant Sci. 146: 9-16. Damiano, C., C. Antonella, C. Emilia, Q. Roverta, and B. Giovanna. 1991. Some factors effecting the induction and the expression of rooting in different fruit species in vitro. Acta Hort. 300: 211-224. Dare, K. S. C., T. D. Oberley and K. E. Mouse. 1994. Expression of manganese superoxide dismutase promoted cellular differentiation. Free Radic. Biol. Med. 16: 275-282. Das, D. K., P. N. Shiva, and N. Bhalla-Sarin. 1999. Multiple shoot induction and plant regeneration in lichi (Lichi chinensis Sonn.). Plant Cell Rep. 18(7/8): 691-695. Debergh, P. C. and L. J. Maene. 1981. A scheme for commercial propagation of ornamental plant by tissue culture. Scientia Hort. 14:335-345. Dimassi, K., V. Chouliaras, G. Diamantidis, and I. Therios. 2003. Effect of iron and auxins on peroxidase activity and rooting performance of three citrus rootstocks in vitro. J. Plant. Nutr. 26 (5):1023-1034. Dodds, J. H. 1982. Experiment in plant tissue culture. Cambridge University Press: 45-49. Dong, L. Z., X. Y. Wang, W. Lu and G. C. Zheng. 2003. Plant regeneration via organogenesis from adventitious bud explants of a medicinal herb species, Polygonatum cyrtonema. In Vitro Cellular & Developmental Biology - Plant. 39 (1): 24-27. Duran-Vila, N., Y. Gogorcena, V. Ortega, J. Ortiz, and L. Navarro. 1992. Morphogenesis and tissue culture of sweet orange (Citrus sinensis (L.) Osb.): Effect of temperature and photosynthetic radiation. Plant Cell Tissue Organ Cult. 29: 11-18. Faria, R. T., and R. D. Illg. 1995. Micropropagation of Zingiber spectabile Griff. Scientia Hort. 62: 135-137. Fukui, H., M. Sugiyama, and M. Nakamura. 1989. Shoot tip culture of Japanese persimmon (Diospyros Kali Thunb.). J. Japan. Soc. Hort. Sci. 58 (1): 43-47. Gamborg, O. L., T. Murashige, T. A. Thorpe and I. K. Vasil. 1976. Plant tissue culture media. In Vitro. 12: 473-478. Garcia, J. L., J. Troncoso, R. Sarmiento, and A. Troncoso. 2002. Influence of carbon source and concentration on the in vitro development of olive zygotic embryo and explants raised from them. Plant Cell Tissue Organ Cult. 69: 95-100. George, E. F. 1993. Plant propagation by tissue culture. I. The technology. Exegetics Ltd., England. George, E. F. and P. D. Sherrington. 1984. Plant propagation by tissue culture: Handbook and dictionary of commercial laboratories, (eds. By George, E. F. and P. D. Sherrington.) Eastern Press Reading, Berk, England: 125-330. Gibson, S. I. 2000. Plant sugar-response pathways. Part of a complex regulatory web. Plant Physiol. 124: 1532-1539. Grete, G. P., R. Oi, A. Gissinger and R. Moe. 2001. Plant morphology is affected by light quality selective plastic films and alternating day and night temperature. Gartenbauwissenschaft. 66: 53-60. Hausman, J. F. 1993. Changes in peroxidase activity, auxin level and ethylene production during root formation by poplar shoots raised in vitro. Plant Growth Regul. 13: 263-268. Hoshino, Y. and M. Mii. 1998. Bialaphos stimulates shoot regeneration from hairy roots of snapdragon (Antirrhinum majus L.) transformed by Agrobacterium rhizzogenes. Plant Cell Rep. 17: 256-261. Hossain, M., M. R. Karim, R. Islam and O. I. Joarder. 1993. Plant regeneration from nucellar tissues of Aegle marmelos through organogenesis. Plant Cell Tissue Organ Cult. 34: 199-203. Hu, C. Y. and P. J. Wang. 1983. Meristem, shoot-tip and bud culture. Handb. Plant Cell Tissue Organ Cult. 1: 177-277. Huang, L. and T. Murashige. 1977. Plant tissue culture media: major constituents; their preparation and some applications. Tissue culture Assoc. Manual. 3: 539-548. Hughes, K. W. 1981. In vitro ecology: Exogenous factors affecting growth and morphogenesis in plant culture systems. Environ. Exp. Bot. 21:281-288. Hussey, G. 1980. In vitro propagation. Tissue culture for Plant pathologists. Oxford: Blackwell Scientific.: 51-61. Hussey, G. 1983. In vitro propagation of horticultural and agricultural crops. Plant Biotechnology. Cambridge University Press.:111-183. Isutsa, D. K. 2004. Rapid micropropagation of passion fruit (Passiflora edulis Sims.) varieties. Scientia Hort. 99: 395-400. Jha, S. and S. Sen. 1985. Regeneration and rapid multiplication of Bowiea volubilis Harv. In tissue culture. Plant Cell Rep. 4: 14-14. Jha, S., and T. B. Jha. 1989. Micropropagation of Cephaelis ipecacuanha Rich. Plant Cell Rep. 8: 437-439. Jones, O. P. 1991. The role of biotechnology in the multiplication and improvement of wood plant. Acta Hort. 289: 35-43. Kadota, M., K. Imizu, and T. Hirano. 2001. Double-phase in vitro culture using sorbitol increases shoot proliferation and reduces hyperhydricity in Japanese pear. Scientia Hort. 89: 207-215. Karam N. S. and M. Al-Majathoub. 2000. In vitro shoot regeneration from mature tissue of wild Cyclamen persicum Mill. Sci. Hort. 86: 323-333. Kim, J. H., R. E. Glick and A. Melis. 1993. Dynamics of photosystem stoichiometry adjustment by light quality in chloroplasts. Plant Physiol. 102: 181-190. Klerk, G. J. D., J. T. Brugge, and S. Marinova. 1997. Effectiveness of indoleacetic acid, indolbuteric acid and naphthaleneacetic acid during adventitious root formation in vitro in Malus ‘Jork9'. Plant Cell Tissue Organ Cult. 49: 39-44. Koblitz, H., D. Koblitz, H. P. Schmauder and D. Groger. 1983a. Studies on tissue cultures of the genus Cinchona L. alkaloid production in cell suspension cultures. Plant Cell Rep. 2: 122-125. Koblitz, H., H. Koblitz, H. P. Schmauder and D. Groger. 1983b. Studies on tissue cultures of the genus Cinchona L. alkaloid production in cell suspension cultures. Plant Cell Rep. 2: 95-97. Kotsias, D. and P. A. Roussos. 2001. An investigation on the effect different plant growth regulating compounds in vitro shoot tip and node culture of lemon seedlings. Scientia Hort. 89: 115-128. Kozai, T. 1991. Photomixotrophic growth of strawberry plantlets in vitro and changes in nutrient composition of the medium. Plant Cell Tissue Organ Cult. 25: 107-115. Kozai, T., C. Kubota, and B. R. Jeong. 1997. Environmental control for the large-scale production of plants through in vitro techniques. Plant Cell Tissue Organ Cult. 51: 49-56. Lal, N., P. S. Ahuja, A. K. Kukreja and B. Pandey. 1988. Clonal propagation of Picrorhiza kurrua Royle ex Benth. by shoot tip culture. Plant Cell Rep. 7: 202-205. Leblay, C., E. Chevraeau, and L.M. Raboin. 1991. Adventitious shoot regeneration from in vitro leaves of several pear cultivars (Pyrus communis L.). Plant Cell Tissue Organ Cult. 25: 99-105. Leontive-Orlov, O. A., L. A. Leontiva-Orlova, A. Mossi, and R. L. Cansian. 2005. Study of the influence of major ions and benzylaminopurin on proliferation in vitro of microshoots of apple, plum, trifoliata orange and black currant by a systematic variance method. Acta Hort. 632:249-253. Liu, L. X., S. Y. Tang, S. M. Xu, F. Sun and Y. Q. Cao. 1993. Effects of different light qualities on structure of chloroplasts and photosynthetic physiological properties in Panax ginseng. Acta Bot. Sin. 35: 588-592. Liu, Y. W., W. Chou and C. Y. Wang. 2005. In viro induction of phosphinothricin tolerance in rice (Oryza sativa). Plant Prot. Bull. 47:47-58. Mao, A. H., A. Wetten, M. Fay and P. D. S. Caligari. 1995. In vitro propagation of Clerodendrum colebrookianum Walp; a potential natural anti-hypertension medicinal plant. Plant Cell Rep. 14: 493-496. Monteuuis, O. and M. C. Bon. 2000. Influence of auxins and darkness on in vitro rooting of micropropagated shoots from mature and juvenile Acacia mangium. Plant Cell Tissue Organ Cult. 63: 173-177. Mor, Y. and N. Zieslin. 1987. Plant growth regulators in rose plants. HortRev. 9: 53-66. Moreira - Dias, J. M., R.V. Molina, J. L Guardiola, and A. Garcia-Luis. 2001. Daylength and photon flux density influence the growth regulator effects on morphogenesis in epicotyl segments of Troyer citrange. Scientia Hort. 87: 275-290. Moreira-Dias, J. M., R.V. Molina, Y. Bordon, J. L. Guardiola, and A.Garcia-Luis. 2000. Direct and indirect shoot organogenic pathways in epicotyl cuttings of Troyer citrange differ in hormone requirements and in their response to light. Ann. Bot. 85: 103-110. Morini, S., M. Trinci, and M. Zacchini. 1991. Effect of different photoperiods on in vitro growth of Mr.S. 2/5 plum rootstock. Plant Cell Tissue Organ Cult. 25: 141-145. Morini, S., R. Sciutti, and P. Fortuna. 1992. In vitro growth response of Prunus cerasifera shoots as influenced by different light-dark cycle and sucrose concentrations. Plant Cell Tissue Organ Cult. 28: 245-248. Mrini, S. and S. Perrone. 2006. Effects of short light-dark regimes on in vitro shoot rooting of some fruit tree rootstocks. Biol. Plant. 50 (3):429-432. Murashige, T. 1974. Plant propagation though tissue cultures. Ann Rev. Plant Physiol. 25: 135-166. Murashige, T. 1977. Manipulation of organ culture in plant tissue cultures. Botanical Bull Acad Sinica. 18: 1-24. Murashige, T. 1978. The impact of plant tissue culture on agriculture. Frontiers of Plant tissue culture. Calgary: Univ. Offset Printing Service:15-26. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 13: 473-497. Naoya, F., K. Y. Mitsuko, U. Masami, T. Kenji and S. Sadanori. 2002. Effects of light quality, intensity and duration from different artificial light sources on the growth of petunia (Petunia hybrida Vilm.). J. Jpn. Soc. Horticult. Sci. 71: 509-516. Nomura, K., S. Matsumoto, K. Masuda, and M. Inoue. 1998. Reduced glutathione promotes callus growth and shoot development in a shoot tip culture of apple root stock M26. Plant Cell Rep. 17: 597- 600. Ochatt, S. J. and H. C. Osvaldo. 1982. In vitro meristem culture of M.4 apple (Malus pumila Mill.). I. Optimal nutrient medium. 2(1): 39-48. Palai, S. K., G. R. Rout and P. Das. 1997. Micropropagation of ginger (Zingiber officinale Rosc.): interaction of growth regulators and culture conditions. Proc. Biotechnology of Spices, Medicinal and Aromatic Plants. Kerala. India: 20-24. Patra, A., B. Rai, G. R. Rout and P. Das. 1998. Successful plant regeneration from callus cultures of Centella asiatiea (Linn.) Urban. Plant Growth Regul. 24: 13-16. Paudyal, K. P. and N. Haq. 2000. In vitro propagation of pummelo (Citrus grandis L.Osbeck). In Vitro Cell. Dev. Biol. Plant. 36: 511-516. Perez-Molphe-Balch, E. and N. Ochoa-Alejo. 1997. In vitro plant regeneration of Mexican lime and mandarin by direct organogenesis. Hort. Sci. 32: 931-934. Phan, C. T. 1991. Vitreous state in vitro culture: ethylene versus cytokinin. Plant Cell Rep. 9: 517-519. Ravishankar, G. A. and L. V. Venkataraman. 1988a. Plant cell culture for high value metabolites. Science Report. 7: 379-384. Rival, A., F. Bernard, and Y.Mathieu. 1997. Changes in peroxidase activity during in vitro rooting of oil palm (Elaeis guineensis Jacq.). Scientia Hort. 71: 103-112. Rodriguez, R., C. Diaz-Sala, L. Cuozzo, and G. Ancora. 1991. Pear in vitro propagation using a double-phase culture system. Hort. Sci. 26: 62-64. Rout, G. R. and P. Das. 1997a. In vitro organogenesis in ginger (Zingiber officinale Rosc.). J. Herbs Species Medicinal Plants. 4 (4): 41-51. Rout, G. R. and P. Das. 1997b. Techniques of micropropagation in vitro. In:Bose TK. Mitra SK. Sadhu MK., Das P., editors. Propagation of Tropical and Sub-tropical Horticultural Crops. Calcutta: Kalyani Publishers: 105-116. Rout, G. R., B. K. Debata and P. Das. 1989. In vitro mass-scale propagation of Rosa hybrida cv. Landora. Curr. Sci. 58 (15): 876-878. Rout, G. R., C. Saxena, S. Samantaray and P. Das. 1999. Rapid clonal propagation of Plumbago zeylanica Linn. Plant Growth Regul. 28: 1-4. Saxena, C., G. R. Rout and P. Das. 1998. Micropropagation of Psoralea corylifolia Linn. J. Med Aromatic Plant Sci. 20: 15-18. Saxena, C., S. K. Palai, S. Samantaray, G. R. Rout and P. Das. 1997. Plant regeneration from callus cultures of Psoralea corylifolia Linn. Plant Growth Regul. 22: 13-17. Shackel, K. A., V. Novello, and E. G. Sutter. 1990. Stomatal function and cuticular conductance in whole tissue-cultured apple shoots. J. Amer. Soc. Hort. Sci. 115 (3): 468-472. Shasany, A. K., S. P. S. Khanuja, S. Dhawan, U. Yaday, S. Sharma and S. Kumar. 1998. High regeneration nature of Mentha arvensis internodes. J. Biosci. 23 (5): 641-646. Shim, S. W., E. J. Hahn, and K. Y. Paek. 2003. In vitro and ex vitro growth of grapevine rootstock ‘5BB' as influenced by number of air exchanges and the presence or absence of sucrose in culture media. Plant Cell Tissue Organ Cult. 75: 57-62. Short, K. C. and A. V. Roberts. Rosa ssp (Roses): in vitro culture, micropropagation and production of secondary products. Biotechnology in Agriculture and Forestry. Vol. 15. Medicinal and Aromatic Plants.III. Berlin: Springer Verlag. 376-397. Singh, S., B. K. Ray, S. Bhattacharyya, and P. C. Deka. 1994. In vitro propagation of Citrus reticulate Blanco and Citrus limon Burm. F. Hort. Science. 29 (3): 214-216. Skirvin, R. M., M. C. Chu, H. J. Young and Rose. 1990. Hand. Plant Cell Cult. New York. MacMillan. 5: 716-743. Smith, E. F., A. V. Roberts, and J. Mottley. 1990. The preparation in vitro of Chrysanthemum for transplantation to soil. Improved resistence to desiccation conferred by paclobutrazol. Plant Cell Tissue Organ Cult. 21: 133-140. Taylor, J. L. S. and J. V. Staden. 2001. The effect of nitrogen and sucrose concentration on the growth of Eucomis autumnalis (Mill.) Chitt. Plantlets in vitro and on subsequent anti-inflammatory activity in extracts prepared from the plantlets. Plant Growth Regul. 34: 49-56. Terwary, P. K. and S. Oka. 1999. Simplified clonal multiplication of mulberry using liquid shake culture. Plant Cell Tissue Organ Cult. 59(3): 223-226. Thomas, J. C., and F. R. Katterman. 1986. Cytokinin activity induced by thidiazuron. Plant Physiol. 81: 681-683. Thomas, P. and M. B. Ravindra. 1997. Shoot tip culture in mango: influence of medium, genotype, explant factors, season and decontamination treatments on phenolic exudation, explant survival and axenic culture establishment. J. Hort. Sci. 72 (5): 713 - 722. Tian, M., Q. Gu and M. Zhu. 2003. The involvement of hydrogen peroxide and antioxidant enzymes in the process of shoot organogenesis of strawberry callus. Plant Sci. 165: 701-707. Tisserart, B. 1981. Date Palm Tissue culture. Oakland, CA: US Department of Agriculture, Agricultural Research Service. Torres, K. C. 1989. Tissue culture Techniques for Horticultural Crops. New York: Van Nostrand Reinhold Co. Unduebo, A. E. 1971. Effect of external supply of growth substances on axillary proliferation and development in Dioscorea bulbifera. Ann. Bot. 35: 159-163. Wakhlu, A. K., S. Nagari and K. S. Barna. 1990. Somatic embryogenesis and plant regeneration from callus cultures of Bunium persicum Boiss. Plant Cell Rep. 9: 137-138. Wang, C. J. and Y. S. Yang. 1999. Studies on shoot tip culture of loquat (Eriobotrya japonica Lindl.) in vitro. Horticulture NCHU (Taiwan). 24 (3): 1- 14. Wang, Z. P., W. Y. Chou and C. Y. Wang. 2005. Roles of ammonium stress, cytokinin and phosphinothricin (PPT) in the induction of rice (Oryza sativa) mircoshoots. Plant Prot. Bull. 47:349-360. Williams, R. R. and A. M. Taji. 1991. Effect of temperature, gel concentration and cytokinins on vitrification of Olearia microdisca (J.M. Black) in vitro shoot cultures. Plant Cell Tissue Organ Cult. 26: 1-6. Withers L. A. and P. G. Anderson. 1986. Plant tissue culture its agricultural applications. London: Butterworths. Xu, H. M. and D. L. Zhao. 2003. Effect of plant growth regulators on several characteristics during in vitro bud regeneration of Polygonatum cyrtonema. Chinese Traditional and Herb Drugs. 34 (9): 855-858. Yancheva, S. D., S. Golubowicz , E. Fisher , S. Yadun, and M. A. Flaishman. 2003. Auxin type and timing of application determine the activation of the developmental program during in vitro organogenesis in apple. Plant Sci. 165: 299 - 309. Yoon, E. S. and Y. E. Choi. 2002. Micropropagation and mass production of adventitious roots of Polygonatum odoratum via the culture of seedling explants. J. Plant Biotec. 4 (1): 33-37. Zacchini, M., S. Morini, and C. Vitagliano. 1997. Effect of photoperiod on some stomatal characteristics of in vitro cultured fruit tree shoots. Plant Cell Tissue Organ Cult. 49: 195-200. Zhou, J., H. Ma, F. Gou and X. Luo. 1994. Effect of thidiazuron on somatic embryogenesis of Cayratia japonica. Plant Cell Tissue Organ Cult. 36: 73-79. Zhou, Y. H., D. P. Guo, Z. J. Zhu and Q. Q. Qian. 2005. Effects of in vitro rooting environments and irradiance on growth and photosynthesis of strawberry plantlets during acclimatization. Plant Cell Tissue Organ Cult. 81: 105-108. Zimmerman, T.W. and R. Scorza. 1994. Benzyladenine and shortened light/dark cycles improve in vitro shoot proliferation of peach. Hort. Sci. 29: 695-698.||摘要:||
大幅增加材料來源。培養基添加3-5 mg/l BA 與 20-40 mg/l
adenine sulfate 明顯提高葉片培養的芽體形成率與芽體數。
3.培養基添加0.1 mg/l PPT更明顯促進葉片基部膨大、芽體形成率
4.芽體培養於含0.1-0.2 mg/l GA，可促進芽體生長，此為誘導發根
6.發根培養基以添加15g/l蔗糖為佳，但若再添加0.5 mg/l GA，則
蔗糖需求上升至25 g/l 為佳，而且發根率與根部生長未受限制，
Huang-Jing, Polygonatum altelobatum Hay., a well known Chinese herbal medicine for thousands years. Papers on tissue culture of P. altelobatum are bare and quite limited. Our study emphasized on effects of plant growth regulators and light conditions on tissue culture of P. altelobatum. The results showed that for initial culture from different parts of mature plant, the optimum concentration of BA at 3.07 mg/l in modified MS medium was highest shoot formation when tuber segments used as explants, and that for young leaf segments was 0.5 mg/l BA. Adenine sulfate also was used in initial culture and the optimum concentration was at 24 mg/l for the highest shoot formation of explants derived from young-leaf explants, and that for tuber explants is at 19.4 mg/l. For shoot multiplication, swollen-base leaf was decreased from 36.7% to 13.3% when phosphinothricin (PPT) concentrations increased from 0 to 0.4 mg/l. The optimum concentration of PPT at 0.11 mg/l got the highest number of shoot in tissue culture, and 0.13 mg/l PPT was optimum concentration for highest axillary bud formation. For shoot growth, GA3 at 0.103 mg/l and 0.095 mg/l were optimum for highest number of shoot formed from bud and swollen-base leaf explants, respectively. Longest shoot occurred when buds and swollen-base leaf used as explants cultured in medium with GA3 at 0.12 mg/l. For root development, root growth response and number of root per explants were significantly increased when the doses of NAA increased regardless of under blue or red light. Optimum concentration of sucrose from 26.75 g/l to 30.5 g/l is highest effective for leaf development and plantlet grew under blue light. The sucrose at 30.31 g/l under red light is suited for the highest number of root in tissue culture of P. altelobatum. The optimum concentration of GA3 at 23.33 g/l to 24.93 g/l is suite for effective of leaf and root development of P altelobatum plantlet.
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