Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/36892
標題: 台灣產藥用植物白花小薊微體繁殖之研究
In Vitro Propagation of Taiwan Medicinal Plant, Cirsium japonicum DC. var. takaoense Kitamura
作者: 王瑞繹
Wang, Jui-Che
關鍵字: 白花小薊;Cirsium japonicum DC. var. takaoense Kitamura;藥用植物;微體繁殖;In Vitro Propagation;Medicinal Plant
出版社: 農藝學系所
引用: 王康才、張雪瓊、茅毓英。2000。杭菊花花瓣組織培養。中草藥 31(8):628-630。 江嘉偉。2006。黃耆組織培養根誘導與成分分析。碩士論文。台中:國立中興大學農藝學系。 沈秀麗、徐仲。1996。甜葉菊組織培養條件的研究 I. 培植體、激素濃度、瓊脂濃度對癒合組織形成及芽體誘導的影響。中國糖料 3:24-26。 沈秀麗、徐仲。1997。甜葉菊組織培養條件的研究 II. 不同基本培養基、不同碳源對癒傷組織形成及芽誘導的影響。中國糖料 4:9-10。 李啟任、彭恒、王東。1997。孔雀菊組織培養快速繁殖研究。雲南大學學報(自然科學版)19(4):370-373。 李璟妤。2005。山防風之微體繁殖。碩士論文。台中:國立中興大學農藝系。 邱年永。1987。大小薊。明通醫藥 132:8-9。 林李昌。2003。何首烏之組織培養。碩士論文。台中:國立中興大學農藝學系。 金洪、張眾、侯占銘、曉紅。1998。水飛薊Silybum marianum (L.) Gaertn.試管苗組織培養研究。內蒙古農牧學院院報 19(3):38-41。 胡文若、陳俊仁。2006。花卉微體繁殖技術。台南區農業專訊 58:1-5。 郝小江、彭麗萍、甘煩遠、胡益明。2001。藥用植物粉花繡線菊的組織培養的建立與快速繁殖研究。中草藥 32(11):1030-1033。 高景輝。1994。植物荷爾蒙生理。台北:華香園出版社。pp. 44-69。 高景輝。1998。植物荷爾蒙生理。台北:華香園出版社。pp. 25-43。 徐雅慧、賴齡、羅吉方、溫國慶、張憲昌。2000。台灣市售大薊、小薊藥材之鑑別。藥物食品檢驗局調查研究年報 18:99-108。 郭昭麟。1986。台灣產薊屬(Cirsium)藥材之生藥學研究。碩士論文。台中:中國醫藥學院中國藥學研究所。 陳西村。1998。神奇野花─白花雞鵤刺。金門季刊 72:48-50。 陳景明。2006。大薊與小薊組織培養大繁殖之研究。碩士論文。台中:國立中興大學農藝學系。 許謙信。1987。菊花莖頂培養。台中區農業改良場研究彙報 16:41-46。 張光雄、邱年永。1992。原色台灣藥用植物圖鑑(3),初版。南天書局。pp. 233-245。台北。 程林梅、高洪文、趙茂林。2002。菊苣組織培養與植株再生的研究。草業學報 11(4):105-107。 黃明華。1997。大薊與小薊品種的本草考證。時珍國醫國藥 8(6):551-552。 黃慧蓮、劉賢旺、謝平、張壽文。2002。菊三七組織培養的研究。中草藥 33(2):159-160。 馮慧心。2004。山防風組織培養及抗氧化活性之研究。碩士論文。嘉義:國立嘉義大學農學研究所。 彭鏡毅、鐘國芳。2000。18.薊屬。出自”台灣維管束簡誌 第四卷”,pp. 243-246。楊遠波、劉和義、彭鏡毅、施炳霖、呂勝由編著。行政院農業委員會出版。台北。 楊樺、鄧小梅、龍蔚。2000。菊三七的組織培養。江西林業科技 6:11-12。 趙振玲、肖植文、劉其寧、錢建寧。2004。滇大薊種苗工廠化生產。西南農業學報 17(4):518-520。 鄭佳綺。2006。茴香菖蒲多芽體誘導及增殖之研究。碩士論文。台中:國立中興大學農藝學系。 劉思謙。2004。大自然的綠精靈─植物。高雄市九十三學年度高級中學生物科師生野外研習活動計劃。 劉寧平。2001。大薊與小薊的鑑別。基層中藥雜誌 16(4):40-41。 蔡新聲。1982。組織培養技術之研究及應用。中華農學會報 120:9-21。 蔡新聲、李鎮宇、蕭翌柱、郭昭麟。2003。台灣珍稀藥用植物利用組織培養之繁殖技術。出自”中草藥產業研發與技術(中草藥教學資源中心主編)”,pp. 278-279。教育部。台北。 賴建宏。2007。菘藍無性繁殖之研究。碩士論文。台中:國立中興大學農藝學系。 薛建平、張愛民、盛瑋、趙豐蘭。2002。安徽藥菊莖尖組織培養技術的研究。中國中藥雜誌 27(5):350-352。 Annadana, S., W. Rademaker, M. Ramanna, M. Udayakumar and J. de Jong. 2000. Response of stem explants to screening and explant source as a basis for methodical advancing of regeneration protocols for chrysanthemum. Plant Cell Tiss. Org. Cult. 62:47-55. Arnold, S. V. and T. Eriksson. 1984. Effects of agar concentration on growth and anatomy of adventitious of Picea abies L. Plant Cell Tiss.Org. Cult. 3:257-264. Ben-Jaacov, J. and R. W. Langhans. 1972. Rapid multiplication of Chrysanthemum plant by stem-tip proliferation. HortScience. 7(3):289-290. Benjamin, B. D. and P. C. Roja. 1987. Multiple shoot cultures of Atropa belladonna: Effect of physico-chemical factors on growth and alkaloid formation. Journal Of Plant Physiology 129(1-2):129-136. Berrios, E. F., L. Gentzbittel, H. Serieys, G. Alibert and A. Sarrafi. 1999. Influence of genotype and gelling agents on in vitro regeneration by organogenesis in sunflower. Plant Cell Tiss. Org. Cult. 59:65-69. Bhattacharya, P., S. Dey, N. Das and B.C. Bhattacharya. 1990. Rapid mass propagation of Chrysanthemum morifolium by callus derived from stem and leaf explants. Plant Cell Rep. 9: 439-442. Carnes, M. G. and M. S. Wright. 1988. Endogenous hormone levels of immature corn kernels of A188, Missouri-17 and Dekalb XL-12. Plant Sci. 57:195-203. Chand, S., A. K. Sahrawat and D. V. S. S. R. Prakash. 1997. In vitro culture of Pimpinella anisum L. (anise). J. Plant Bioch. & Biotech. 6:1-5. Chen, H. and M. A. Maun. 1998. Population ecology of Cirsium pitcheri on Lake Huron sand dunes. III. Mechanisms of seed dormancy. Can. J. Bot. 76:575-586. Choffe, K. L., S. J. Murch and P. K. Saxena. 2000. Regeneration of Echinacea purpurea: Induction of root organogenesis from hypocotyls and cotyledon explants. Plant Cell Tiss. Org. Cult. 62:227-234. Chu, C. C., C. S. Wang, C. C. Sun, C. Hiu, K. C. Yin and C. Y. Chu. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiment on the nitrogen source. Sci. Sinica 18:659-668. Drew, R.A., J.A. McComb and J.A. Considine. 1993. Rhigogenesis and root growth of Carica papaya in vitro in relation to auxin sensitive phases and use of riboflavin. Plnat Cell Tiss. Org. Cult. 33:1-7. D’Silva, I. and L. D’Souza. 1992. In vitro propagation of Anacardium occidentale L. Plant Cell Tiss. Org. Cult.29:1-6. Dunbar, K. B. and R. N. Pittman. 1992. Adventitious shoot formation from mature leaf explants of Archis species. Crop Sci. 32:1353-1356. Economou, A.S. and P. E. Read. 1987. Light treatments to improve efficiency of in vitro propagation systems. Hort. Sci. 22(5):751-754. Edwin, F. G. 1993. Plant propagation by tissue culture. 2nd ed. England: Exegetics Ltd. Fujii, Y. and K. Shimizu. 1990. Regeneration of plants from achenes and petals of Chrysanthemum coccineum. Plant Cell Rep. 8: 625-627. Fukai, S. 1986. Effects of sugar on callus and organ formation from leaf segments of chrysanthemum (Dendranthema grandiflorum Kitamura). Bull Osaka Agric. Res. Cent. 3:71-77. Gamborg, O. L., R. H. Miller and K. Ojima. 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:148-151. Gao, Y., B. Zhao, G. Ding and Q. Zhang. 2001. Shoot regeneration from stem and leaf explants of Dendranthema grandiflorum. J. Beijing Forestry Univ. 23:32-33. George, E. F. and P.D. Sherrington. 1984. Plant Propagation by Tissue Culture. Handbook and Directory of Commercial Laboratories. Ed. England: Eastern Press, Reading, Berks. pp. 125-330. Hill,G. P. 1968. Shoot formation in tissue cultures of Chrysanthemum ‘Bronze Pride’. Physiol. Plant. 21:386-389. Hosoki, T. and T. Nagasako. 1996. In vitro propagation of plumed thistle (Cirsium japonicum DC. cv. Teraoka) by vertical shoot-split method. Plant Tiss. Cult. Let. 13(2):173-176. Jäger, E. 1977. Wuchsform und Verbreitung der Cirsium acaule Verwandtschaft in Eurasien. Flora 166:75-92. Jaime, A. and T. de Silva. 2003. Chrysanthemum: advances in tissue culture, cryopreservation, postharvest technology, genetics and transgenic biotechnology. Biotech. Adv. 21:715-766. Kaul, V., R. M. Miller, J. F. Hutchinson and D. Richards. 1990. Shoot regeneration from stem and leaf explant of Dendranthema grandiflora Tzvelev (syn. Chrysanthemum morifolium Ramat.). Plant Cell Tiss. Org. Cult. 21:21-30. Komamine, A., R. Kawahara,M. Matsumoto, S. Sunabori, T. Toya, A.Fujiwara, M. Tsukahara, J. Smith, M. Ito, K. Nomura and T. Fujimura. 1992. Mechanisms of somatic embryogenesis in cell cultures : physiology, biochemistry, and molecular biology. In vitro Cell. Dev. Biol. 28:11-14. Lane, W. D. 1979. In vitro propagation of Spirea bumalda and Prunus oistena from apices. Can. J. Plant Sci. 50:1025-1029. Lee, T., H. E. E. Michele, and P. Eng- Chong. 1997. High frequency shoot regeneration from leaf disc explants of garland chrysanthemum (Chrysanthemum coronarium L.) in vitro. Plant Sci. 126:219-226. Lu, C. Y. 1993. The use of thidiazuron in tissue culture. In Vitro Cell Dev. Biol.-Plant. 29:92-96. Lu, C. Y., G. Nugent and T. Wardley. 1990. Efficient, direct plant regeneration from stem segments of chrysanthemum (Chrysanthemum morifolium Ramat. cv. Royal Purple). Plant Cell Rep. 8:733-736. Malaure, R. S., G. Barclay, J. B. Power and M. R. Davey. 1991. The production of novel plants from florets of Chrysanthemum morifolium using tissue culture. I. Shoot regeneration from ray florets and somaclonal variation exhibited by the regenerated plants. J. Plant Physiol. 139:8-13. May, R. A. and Trigiano R. N. 1991. Somatic embryogenesis and plant regeneration from leaves of Dendrathema grandiflora. J. Am. Soc. Horrt. Sci. 116:366-371. Meusel, H. and E. Jäger. 1992. Vergleichende Chorologie der Zentraleuropaischen Flora. Band III. Karten. Stuttgart and New York: Gustav Fischer Verlag Jena. Miyazaki, S. and Y. Tashiro. 1978. Tissue culture of Chrysanthemum morilifolium. IV. Explant sources for stem segment culture. Agr. Bull. Saga Univ. 44:67-78. Miyazaki, S., Y. Tashiro and T. Shimada. 1976. Tissue culture of Chrysanthemum morifolium Ramat. I. Cultivar differences in organ formation. Agr. Bull. Saga Univ. 40:31-44. Murashige, T. 1977. Manipulation of organ initiation in plant tissue cultures. Bot. Bull. Acad. Sinica 18:1-24. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassys with tobacco tissue culture. Physiol. Plant. 15:437-479. Nigra, H. M., M. A. Alvarez and A. M. Giulietti. 1989. The influence of auxins, light and cell differentiation on solasodine production by Solanum eleagnifolium Cav. Calli. Plant Cell Rep. 8:230-233. Ozias, A. P. and I. K. Vasil. 1985. Cell culture and somatic cell genetics of plants. pp. 129-147. I. K. Vasil (ed.), Accdemic Press, New York. Patra, A., B. Rai, G. R.Rout and P. Das. 1998. Successful plant regeneration from callus cultures of Centella asiatica (Linn.) Urban. Plant Growth Regul. 24:13-16. Prasad, R.N. and H. C. Chaturvedi. 1988. Effect of season of collectin of explants on micropropagation of Chrysanthemum morifolium. Bio. Plant. 30(1):20-24. Ritchie, G. A., K. C. Short and M. R. Davey. 1991. In vitro acclimatization of chrysanthemum and sugar beet plantlets by treatment with paclobutrazol and exposure to reduced humidity. J. Exp. Bot. 42:1557-1563. Roest, S. and G. S. Bokelmann. 1975. Vegetative propagation of Chrysanthemum morifolium Ram. In vitro. Sci. Hort. 3:317-330. Romano, A., C. Noronha and M. A. Martins-Loucao. 1995. Role of carbohydrates in micropropagation of cork oak. Plant Cell Tiss. Org. Cult. 40:159-167. Rout, G. R. and P. Das. 1997. Recent trends in the biotechnology of Chrysanthemum: a critical review. Sci. Horti. 69:239-257. Rout, G. R., C. Saxena, S. Samantaray and P. Das. 1996. Rapid clonal propagation of Plumbago zeylanica Linn. Plant Growth Regul. 28:1-4. Sauvadet, M. A., P. Brochard and J. Boccon-Gibod. 1990. A protoplast-to-plant system in Chrysanthemum: differential responses among several commercial clones. Plant Cell Rep. 8:692-695. Seo, S. Y., C. Dong-Chil, K. Jeong-Man, L. Hoe-Chun, K. Hee-Jun, C. Joung-Sik and C. Yeong-Geun. 2003. Plant regeneration from leaf explant and efficient Agrobacterium-mediated transformation system of chrysanthemum (Dendranthema grandiflorum). Acta Hort. 620:333-339. Shibli, R. A., M. A. L. Smith and L. A. Spomer. 1992. Osmotic adjustment and growth responses of three Chrysanthemum morifolium Ramat. cultivars to osmotic stress induced In Vitro. J. Plant Nutri. 15(9):1373-1381. Skoog, F. and C. O. Miller. 1957. Chemical regulation of growth and organ formation in plant tissue culture in vitro. Symp. Soc. Exp. Biol. 11:118-131. Tanaka, K., Y. Kanno, S. Kudo and M. Suzuki. 2000. Somatic embryogenesis and plant regeneration in chrysanthemum (Dendranthema grandiflorum (Ramat.) Kitamura). Plant Cell Rep. 19:946-953. Thorpe, T. A. 1980. Plant tissue culture: methods and application in agriculture. pp. 379., New York: Academic Press. Vrata, E, F, and F. W. Went. 1949. Some pH changes in nutrient solutions Bot. Gaz. 110:605- 613. Webb, K. J. and H. E. Street. 1977. Morphogenesis in vitro of Pinus and Picea. Acta Hort. 78:259-269. White, P. R. 1943: A handbook of plant tissue culture. New York, Ronald Press Co.
摘要: 
本研究以白花小薊無菌苗之葉片與莖節培植體為材料,進行不同MS培養基濃度、蔗糖濃度、NAA配合BA或TDZ濃度組合對癒合組織形成及不定芽誘導之影響。結果顯示葉片培養於1/2MS和MS培養基可獲得較高的癒合組織形成率(分別為83.0%和83.3%)、不定芽形成率(分別為65.3%和68.1%)、優良的不定芽再生數量,莖節培養亦於1/2 MS培養基可獲得明顯較高的不定芽形成率(77.3%)和不定芽再生數量(5.2株),較葉片培植體最高之芽數(4.9株)為高,且不定芽生長亦較為碩大健壯。葉片和莖節培養於不同濃度蔗糖之試驗均以添加30 g/L蔗糖之1/2 MS培養基,不定芽再生數均為4.8株較其他濃度為高,此時的癒合組織形成率均為86.7%、不定芽形成率分別為69.4%和84.7%亦最高。在生長調節劑組合試驗中,添加0.5 mg/L NAA配合0.2 mg/L BA、0.2 mg/L NAA配合0.5 mg/L TDZ的1/2 MS培養基對葉片培植體,以及添加0.2 mg/L NAA配合0.5 mg/L BA、或0.5 mg/L TDZ對莖節誘導不定芽再生效果最佳。然而再生芽體之生長則以培養於添加BA處理的不定芽葉片較圓潤,呈現長橢圓形至卵形,培養於添加TDZ處理的不定芽葉片多呈現扭曲狹長的披針形。1/2 MS培養基中添加0.25 mg/L或0.5 mg/L NAA配合0.25 mg/L BA、0.5 mg/L NAA配合0.25 mg/L TDZ對促進單一芽體發根情形明顯優於其他NAA濃度處理。再將已發根且生長良好之0.25 mg/L NAA配合0.25 mg/L BA及0.5 mg/L NAA配合0.25 mg/L TDZ處理之白花小薊組培苗種植於真珠石混合泥炭土(1:1)中1個月後,NAA配合BA處理之存活率100%,且植株葉片呈現長橢圓形至卵圓形,生長勢均一穩定,NAA配合TDZ處理之植株存活率85%,植株葉片呈現細狹之披針狀,生長勢較弱且不一致。

Studies on callus induction, adventitious shoot regeneration of Cirsium japonicum DC. var. takaoense Kitamura using leaf and stem-node as explants were conducted with different strength of MS medium, sucrose concentration and growth regulator combination in this investigation. The highest callus inductive rate 83.0% and 83.3%, shoot regeneratin rate 65.3% and 68.1% and number of adventitious shoots of leaf explants were obtained on 1/2 MS and MS medium respectively. The highest adventitious shoot regeneration rate (77.3%) and mean number of adventitious shoots (5.2) were also obtained on 1/2 MS medium for stem-node xplants. Shoot growth of stem-node explants was the best on 1/2 MS medium than that of others. The highest adventitious shoot numbers of 4.8 with leaf and stem-node explants were cultured on 1/2 MS medium with 30 g/L sucrose. Same tendency for callus inductive 86.7% and adventitious shoot regeneration rate of 69.4% and 84.7% was found for leaf and stem-node explants on 30 g/L sucrose treatment. The 1/2 MS medium with 0.5 mg/L NAA and 0.2 mg/L BA, 0.2 mg/L NAA and 0.5 mg/L TDZ were found the best combination of growth regulators on adventitious shoot regeneration from leaf explants. Meantime, 1/2 MS medium with 0.2 mg/L NAA and 0.5 mg/L BA or 0.5 mg/L TDZ were found the best treatments among tests on adventitious shoot regeneration from stem-node explants. Observation of shoot growth derived from BA treatments had leaf with oval and smooth shape, however leaf of explants derived from TDZ treatment showed baguette and contorted shape. The best root formation was found on 1/2 MS medium containing with 0.25 mg/L, 0.5 mg/L NAA and 0.25 mg/L BA or 0.5 mg/L NAA and 0.25 mg/L TDZ. Acclimation rate of in vitro seedling derived from 0.25 mg/L NAA and 0.25 mg/L BA or 0.5 mg/L NAA and 0.25 mg/L TDZ on medium of perlite and peta-moss (1 : 1) had 100% and 85% after one month culturing, respectively. It was found that plantlets grow healthy with normal oval leaf shape derived from BA treatments. However, weak growth of plantlet with baguette and contorted leaf were found onshoot derived from TDZ treatments.
URI: http://hdl.handle.net/11455/36892
其他識別: U0005-1508200811211800
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