Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/36898
DC FieldValueLanguage
dc.contributor王仕賢zh_TW
dc.contributorShyh-Shyan Wangen_US
dc.contributor陳惠美zh_TW
dc.contributorHuei-Mei Chenen_US
dc.contributor.advisor古新梅zh_TW
dc.contributor.advisorHsin-Mei Kuen_US
dc.contributor.author陳威宇zh_TW
dc.contributor.authorChen, Wei-Yuen_US
dc.contributor.other中興大學zh_TW
dc.date2009zh_TW
dc.date.accessioned2014-06-06T07:58:11Z-
dc.date.available2014-06-06T07:58:11Z-
dc.identifierU0005-1908200810141800zh_TW
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Bot. 85:607-612 McClure R. A. 1966. The bamboos: a fresh perspective. Harvard University Press, Cambridge, Mass McCouch S. R., X. Chen, O. Panaud, S. Temnykh, Y. Xu, Y. G. Cho, N. Huang, T. Ishii, and M. Blair. 1997. Microsatellite marker development, mapping and applications in rice genetics and breeding. Plant Mol. Biol. 35:89-99 McCouch S. R., L. Teytelman, Y. Xu, K. B. Lobos, K. Clare, M. Walton, B. Fu, R. Maghirang, Z. Li., Y. Xing, Q. Zhang, I. Kono, M. Yano, R. Fjellstrom, G. DeClerck, D. Schneider, S. Cartinhour, D. Ware, and L. Stein. 2002. Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Res. 9:199-207 Nayak S. and G. R. Rout. 2005. Isolation and characterization of micro satellites in Bambusa arundinacea and cross species amplification in other bamboos. Afr. J. Biotechnol. 4:151-156 Ohrnberger D. 1999. The bamboos of the world. Elsevier, Amsterdam Ohrnberger D. and J. Goerrings. 1986. The bamboos of the world. Odenthal, FRG Peakall R., S. Gilmore, W. Keys, M. Morgante, and A. Rafalski. 1998. Cross-species amplification of soybean (Glycine max) simple sequence repeats (SSRs) within the genus and other legume genera: implications for the transferability of SSRs in plants. Mol. Biol. Evol. 15:1275-1287 Provan J. 2000. Novel chloroplast microsatellites reveal cytoplasmic variation in Arabidopsis thaliana. Molecular Ecology 9:2183-2185 Ramanayake S. M. S. D., V. N. Meemaduma, and T. E. Weerawardene. 2007. Genetic diversity and relationships between nine species of bamboo in Sri Lanka, using random amplified polymorphic DNA. Pl. Syst. Evol. 269:55-61 Roldán-Ruiz I., J. Dendauw, E. Van Bockstaele, A. Depicker, and M. De Loose. 2000. AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Molecular Breeding 6:125-134 SanMiguel P., A. Tikhonov, and Y. K. Jin. 1996. nested retrotransposons in the intergenic regions of the maize genome. Science 274:765-768 Seethalakshmi K. K. and M. S. M. Kumar. 1998. Bamboos of India-a compendium. INBAR, Beijing Smulders M. J. M., G. Bredemeijer, W. Rus-Kortekaas, P. Arens, and B. Vosman. 1997. Use of short microsatellites from database sequences to generate polymorphisms among Lycopersicon esculentum cultivars and accessions of other Lycopersicon species. Theor. Appl. Genet. 97:264-272 Wierdl M., M. Dominska, and T. D. Petes. 1997. Microsatellite instability in yeast: dependence on the length of the microsatellite. Genetics 146:769-779 Zhu J. F. 1985. Bamboo development in China. In: Rao A. N., G. Dhanarajan, and C. B. Sastry. Recent research on bamboos - proceedings of the international bamboo workshop October 6-14. Hangzhou, China. 24-25. Hangzhou, People's republic of China. The Chinese Academy of Forestry, China / International Development Research Centre, Canada http://www.ytower.com.tw/prj/prj_236/p1.aspen_US
dc.identifier.urihttp://hdl.handle.net/11455/36898-
dc.description.abstract竹子屬於禾本科中的竹亞科,竹子由於生殖性狀資訊缺乏、營養性狀易受環境影響,因此,現今越來越多的試驗由 DNA 分子研究的觀點切入,希望能克服傳統以性狀進行分類的問題。本論文選擇包含台灣特有種在內的22個竹子品種及一個水稻品種作為試驗材料,運用跨物種 SSR 應用的方式,以水稻12條染色體上平均分布的120個 SSR 分子標誌分析竹類之基因組。在獲 PCR 產物的 SSR 引子 (80/120, 66.67%)、多型性比例 (100%)、平均每個 SSR 得到的 allele 數目 (27.4) 等方面,結果皆較前人文獻中高,但不同種類的 motif 則對增幅成功率影響不大。分群分析的結果將水稻分在最外圍,以相似係數0.06把22個竹子品種分成三群,並搭配地理分布區域及地下莖生長方式,分析出22個竹子品種間之關係。zh_TW
dc.description.abstractBamboo belong to Bambusoideae, Gramineae. Information of bamboo flowering material is rare and vegetative characters are often environmentally influenced. Accordingly, more study use molecular markers to solve the problem derived from taxonomy of bamboos using vegetative characters. In this study, we used 120 rice (Oryza sativa) simple sequence repeat (SSR) markers in 22 bamboo species and one rice, O.sativa cv TNG67, as out group control. The result indicated that, 66.67% (80/120) primer pairs yielded products and were polymorphic in bamboo species, and the average number of alleles produced per primer set was 27.4. These result were better than in previous studies. But the primer amplification was not affected by different motif types. In the analysis dendrogram, rice was a outgroup and the 22 bamboo species separated into three clusters by similarity 0.06. According to geographic location and growing type (clumper or runner), these result supported the current bamboo division and provided valuable information about phylogenetic relationships.en_US
dc.description.tableofcontents中文摘要………………………………………………………………i 英文摘要………………………………………………………………ii 目錄……………………………………………………………………iii 圖目錄…………………………………………………………………v 表目錄…………………………………………………………………vi 一、前言………………………………………………………………1 二、前人研究…………………………………………………………3 (一) 竹子簡介與分布情形………………………………………3 (二) 竹子用途分類與重要性……………………………………3 1. 食用品種…………………………………………4 2. 木材品種…………………………………………4 3. 編織品種…………………………………………4 4. 園藝品種…………………………………………5 5. 造紙品種…………………………………………5 (三) 竹子的分類………………………………………………6 (四) 分子標誌輔助竹子品種鑑定與分析親緣關係…………7 1. DNA序列差異………………………………………8 2. RFLP (Restriction Fragment Length Polymorphism)..8 3. RAPD (Random Amplified Polymorphic DNA)...…9 4. AFLP (Amplified Fragment Length Polymorphism)..10 5. SSR (Simple Sequence Repeat)…………………11 (五) 跨物種 SSR 的利用……………………………………11 1. 跨品種間 SSR的利用……………………………11 2. 跨物種間 SSR的利用……………………………12 3. 跨屬間 SSR 的利用……………………………12 4. 綜合跨屬種間 SSR 之利用……………………12 5. 單子葉禾本科跨物種 SSR 的利用.........................13 三、材料與方法………………………………………………………15 (一) 試驗材料………………………………………………15 (二) 萃取竹子總量 DNA…………………………………15 (三) SSR 分子標誌分析………………………………………16 (四) 聚丙醯胺膠體電泳分析與銀染法顯影..............................17 (五) 分群分析………………………………………………18 四、結果………………………………………………………………20 (一) SSR 分子標誌分析……………………………………20 (二) 分群分析………………………………………………21 五、討論………………………………………………………………23 (一) SSR 分子標誌分析……………………………………23 1. 不同 SSR 來源在不同物種能 PCR 增幅的比例 ............................................................................................23 2. 具多型性之 SSR 比例...........................................24 3. 不同種類 motif…………………………………24 4. 每個 SSR 所獲得之 allele 數目...........................25 (二) 分群分析…………………………………………………25 1. 相似係數…………………………………………26 2. I 群之分群分析…………………………………26 3. II 群之分群分析………………………………27 4. III 群之分群分析………………………………28 5. 不同地域性起源與竹子品種間之分群…………29 6. 叢生竹類與散生竹類之分群……………………30 7. 甜竹之定義………………………………………30 六、結論與展望………………………………………………………32 七、參考文 獻…………………………………………………………34 表目錄 頁次 Table 1. The list of material information used in this study..................38 Table 2. Information of rice SSR markers used in this study………39 Table 3. Effect of motif type of rice SSR markers on cross-species amplification in bamboos……………………………………41 (a) Classification by motif structure …………………………41 (b) Classification by motif length………………………………42 Table 4. Amplification efficacy of SSR primers derived from different rice chromosomes……………43 Table 5. Similarity matrix between bamboo species based on Jaccard’s coefficient ...…………………………………………………44 Table 6. Examples of SSR cross-species amplification in previous studies………………………………………………………45 圖目錄 頁次 Fig. 1 A representative SSR profile of 23 species amplified with RM36 & RM503………………………………………………………46 Fig. 2 Dendrogram derived from UPGMA cluster analysis based on (a) 2196 polymorphic alleles of 22 bamboos and one rice species…47 (b) geographic location………………………………………48 (c) growing type: Clumper and Runner…………………………49zh_TW
dc.language.isoen_USzh_TW
dc.publisher農藝學系所zh_TW
dc.relation.urihttp://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1908200810141800en_US
dc.subjectbambooen_US
dc.subject竹子zh_TW
dc.subjectcross-speciesen_US
dc.subjectSSRen_US
dc.subjectevolutionen_US
dc.subject跨物種zh_TW
dc.subjectSSRzh_TW
dc.subject演化zh_TW
dc.title利用水稻簡單序列重複分子標誌分析竹子遺傳變異zh_TW
dc.titleApplication of Rice SSR Markers on Bamboo Genetic Diversityen_US
dc.typeThesis and Dissertationzh_TW
item.openairetypeThesis and Dissertation-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.languageiso639-1en_US-
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