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標題: 巴拉刈抗性水稻突變體之抗氧化反應
Antioxidative response of paraquat resistant rice mutants
作者: 張育綺
Chang, Yu- Chi
關鍵字: paraquat;水稻;rice;TNG67;antioxidative;malondialdehyde;MDA;ascorbate;superoxide dismutase;SOD;ascorbate peroxidase;APX;glutathione reductase;GR;GSH/GSSG;台農67號;抗氧化;丙二醛;抗壞血酸;超氧歧化酶抗壞血酸過氧化酶穀胱甘肽還原酶
出版社: 農藝學系所
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本試驗探討由台農67號(TNG 67)經疊氮化鈉所誘導的巴拉刈抗性突變體其抗性生理機制中抗氧化系統所扮演之角色。藉由光系統Ⅱ光化學最大效能值作為判斷標準,顯示在100 μM巴拉刈處理後抗性與感性水稻植株其抗性具有顯著差異。測定植物體脂質過氧化傷害產物丙二醛(malondialdehyde, MDA)作為植物體氧化傷害之指標,結果指出以100 μM巴拉刈處理9小時後,抗性植株之MDA累積量僅增加40%,而感性植株則增加為130%,顯示抗性植株對於巴拉刈所造成之氧化逆境有較佳抗性。進一步測定植株內抗氧化相關酵素,如超氧歧化酶(superoxide dismutase, SOD)、抗壞血酸過氧化酶(ascorbate peroxidase, APX)與穀胱苷肽還原酶(glutathione reductase, GR)活性的結果顯示,以100 μM巴拉刈處理3小時,感性植株其SOD活性逐漸下降,之後並維持在較低的活性,因此可能造成植株內超氧陰離子的累積,提高植物體內的氧化逆境。此外,在巴拉刈處理後抗性植株APX活性有上升的趨勢;感性植株則沒有顯著增加,並且在整個試驗過程中,感性植株其抗壞血酸鹽(ascorbate)均維持在較低的含量。
在巴拉刈處理後抗性植株其GR活性快速而顯著增加,且感性植株大幅下降,此一結果顯示活化的reduced glutathione/oxidized glutathione (GSH/GSSG) cycle在水稻巴拉刈抗性突變體中具有關鍵角色。此種現象也存在於野茼蒿抗性生物型(Chiang et al. 2008)。本研究進一步藉由外施抗壞血酸試驗探討GR活性對於增強水稻抗巴拉刈的貢獻。

In order to explore the physiological mechanism of paraquat resistance of rice mutant, a sodium azide-mutated rice of cv. Tainung 67 (TNG 67) in Taiwan, whose resistance to this herbicide has been identified, and the role of antioxidative system was assessed. The susceptible (S) and resistant (R) mutants of rice were distinguished clearly by the maximal efficiency of photosystem II photochemistry (Fv/Fm) caused by 100 μM paraquat. While a 40% increment of malondialdehyde, an indicator for peroxidation damage to plant, in R-mutant was observed, that in S-mutant increased significantly by 130% at 9 hours after treatment (HAT) of 100 μM paraquat. Analysis of several antioxidants and pertinent enzymes revealed that superoxide dismutase (SOD) activity was decreased by paraquat 3 HAT in S-mutant and maintained at low activity afterward, which might cause more superoxide anion radicals accumulation. Besides, ascorbate peroxidase (APX) activity obviously increased after paraquat treatment in R-mutant, whereas none of increment in S-mutant; and a significantly low level of ascorbate existed throughout the experiment in S-biotype. Although the higher ratio of reduced glutathione to toal glutathione in R-mutant as compared with S-mutant was found till 9 HAT, coupled with a pronounced and fast increase of glutathione reductase (GR) activity in R-mutant, as well as the large decline of that in S-mutant, suggests that an active reduced glutathione/oxidized glutathione (GSH/GSSG) cycle is critical to paraquat resistance of rice mutant. This phenomenon has also been reported in tall fleabane (Chiang et al. 2008). The decisive contribution of a functional GSH/GSSG cycle to paraquat resistance through an enhancement of GR activity in this rice mutant was further confirmed by an experiment of exogenous application of ascorbate.
其他識別: U0005-0607200916172200
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