Please use this identifier to cite or link to this item:
標題: 蕨叢植株剋它物質之研究
Studies on the allelochemicals of bracken fern (Pteridium aquilinum (L.) Kuhn)
作者: 陳柏蓁
Chen, Bo-Jen
關鍵字: bracken fern;蕨;allelochemical;mitotic index;剋它物質;有絲分裂指數
出版社: 農藝學系所
引用: 郭城孟。1997。臺灣維管束植物簡誌。第壹卷。行政院農業委員會。 Alonso-Amelot, M. E., U. Castillo, B. L. Smith, and D. R. Lauren. 1996. Bracken PTA in milk. Nature 382: 578. Agnew, M. P., and D. R. Lauren. 1991. Determination of ptaquiloside in bracken fern (Pteridium esculentum). J. Chromatogr. 538: 462-468. Burkhalter, P. W., P. M. J. Groux, U. Candrian, P. Hubner, and J. Luthy. 1996. Isolation, determination and degradation of ptaqiiloside—a bracken fern (Pteridium aquilinum) carcinogen. J. Nat. Toxins 5: 141-159. Catillo, U. F., Y. Sakagami, M. Alonso-Amelot, and M. Ojika. 1999. Pteridanoside, the first protoilludane sequiterpene glucoside as a toxic component of the neotropical bracken fern Pteridium aquilinum var. Caudtum. Tetrahedron 55: 12295-12300. Chaniago, I., A. Taji, P. Kristiansen, and R. Jessop. 2008. Soybean root-tip-cell mitosis under the influence of aqueous extracts of three weed species. Jurnal Akta Agrosia 11: 1-6. Dolling, A. H. U., O. Zackrisson, and M. C. Nilsson. 1994. Seasonal variation in phytotoxicity of bracken (Pteridium aquilinum L. Kuhn). J. Chem. Ecol. 20: 3163-3173. Dolling, A. H. U. 1996. Interference of bracken (Pteridium aquilinum L. Kuhn) with Scats pine (Pinus sylvestris L.) and Norway spruce (Picea abies L. Karst.) seedling establishment. For. Ecol. Mange. 88: 227-235. Fenwick, G. R. 1988. Bracken (Pteridium aquilinum )-toxic effects and toxic constituents. J. SCI. Food Agric. 46: 147-173. Ghorbani, J., M. G. Le Duc, H. A. McAllister, R. J. Pakeman, and R. H. Marrs. 2003. Effects of the litter layer of Pteridium aquilinum on seed banks under experimental restoration. Appl. Veg. Sci. (9): 127-136. Ghorbani, J., M. G. Le Duc, H. A. McAllister, R. J. Pakeman, and R. H. Marrs. 2006. Effects of the litter layer of Pteridium aquilinum on seed banks under experimental restoration. Appl. Veg. Sci. 9:127-136. Glissman, S. R. and C. H. Muller. 1978. The allelopathic mechanisms of dominance in bracken (Pteridium aquilinum) in southern California. J. Chem. Ecol. 4: 337-362. Jensen, P. H., O. S. Jacobsen, H. C. B. Hansen, and R. K. Juhler. 2008. Quantification of ptaquiloside and pterosin b in soil and groundwater using liquid chromatography-tandem mass spectrometry (LC-MS/MS). J. Agric. Food Chem. 56: 9848–9854. Lefevre, M., H. Jakob, and M. Nisbet. 1950. Sur la secretion, par certaines cyanophytes. de substances algostaliques dans les collections d''eau naturelles. C. R. Aead. Sci. 230: 2226-2227. Legrand, C., K. Rengefors, G. O. Fistarol, and E. Graneli. 2003. Allelopathy in phytoplanlton – biochemical, ecological and evolutionary aspects. Phycologia 42:406-419. Lioi, M. B., R. Barbieri, G. Borzacchiello, S. Dezzi, S. Roperto,A. Santoro, V. Russo, and F. Roperto. 2004. Chromosome aberrations in cattle with chronic enzootic haematuria. J. Comp. Path. 131: 233–236 Marrs, R. H., and A. S. Watt. 2006. Biological flora of the British Isles: Pteridium aquilinum (L.) Kuhn. J. Ecol. 94: 1271-1321. Marrs, R, H., K. Galtress, C. Tong, E. S. Cox, S. J. Blackbird, T. J. Heyes, R. J. Pakeman, and M. G. L. Duc. 2007. Competing condervation goals, biodiversity or ecosystem services: Element losses and species recruitment in a managed moorland-bracken model system. J. Environ. Manage. 85:1034-1047. Mithchell, R. J., R. H. Marrs, and M. H. D. Auld. 1998. A comparative study of the seedbanks of heathland and successional habitats in Dorset, Southern England. J. Ecol. 86: 588-596. Pakeman, R. J., and E. Hay. 1996. Heathland seedbanks under bracken Pteridium aquilinum (L.) Kuhn and their importance for re-vegetation after bracken control. J. Environ. Manage. 47: 329-339. Pakeman, R. J., M. G. Le Duc, and R. Marrs. 2001. A review of current bracken control and associated vegetation strategies in Great Britain. Wed Ecol. 3: 6-11. Peretti, V., F. Ciotola1, S. Albarella, V. Russo, G. P. Di Meo, L. Iannuzzi, F. Roperto, and V. Barbieri. 2007. Chromosome fragility in cattle with chronic enzootic haematuria. Mutagenesis 22: 317–320. Pywell, R. F., R. J. Pakeman, E. A. Allchin, N. A. D. Bourn, E. A. Warman, K. J. Walker. 2002. The potential for lowland heath regeneration following plantation removal. Biol. Conserv. 108: 247-258. Rasmussen, L. H., S. Kroghsbo, J. C. Frisvad, H. C. B. Hansen. 2003a. Occurrence of the carcinogenic Bracken constituent ptaquiloside in fronds, topsoils and organic soil layers in Denmark. Chemosphere 51: 117-127. Rasmussen, L. H., L. S. Jensen, and H. C. B. Hansen. 2003b. Distribution of the carcinogenic terpene ptaquiloside in bracken fronds, rhizomes (Pteridium aquilinum), and litter in denmark. J. Chem. Ecol. 29: 771-778. Rasmussen, L. H., H. Christian, B. Hansen, and D. Lauren. Sorption, degradation and mobility of ptaquiloside, a carcinogenic Bracken (Pteridium sp.) constituent, in the soil environment. 2005. Chemosphere 58: 823–835. Rice, E. L. 1984. Allelopathy, 2nd edition, Academic Press, Orlando, Floria. Romagni, J. G., S. N. Allen, and F. E. Dayan. 2000. Allelopathic effects of volatile cineoles on two weedy plant species. J. Chem. Ecol. 26: 303-313. Saito, K., T. Nagao, M. Matoba, K. Koyama, S. Natori, T. Murakami, and Y. Saiki. 1989. Chemical assay of ptaquiloside, the carcinogen of Pteridium aquilinum, and the distribution of related compounds in the Pteridaeae. Phytochemistry 28: 1605-1611. Saito, K., T. Nagao, S. Takatsuki, K. Koyama, and S. Natori,. 1990. The sequiterpenoid carcinogen of bracken fern, and some analogues, from the Pteridaeae. Phytochemistry 29: 1475-1497. Shahin, M., B. L. Smith, and A. S. Prakash. 1999. Bracken carcinogens in the human diet. Mutat. Res. 443: 37-44. Smith, B. L., A. A. Seawright, J. C. Ng, A. T. Hertle, J. A. Thomson, and P. D. Bostock. 1994. Concentraction of ptaquiloside, a major carcinogen in bracken fern (pteridium spp.), from Eastern Australia and from a cultivated worldwide collection held in Sydney, Australia. Nat. Toxins 2: 347-353. Stewart, R. E. 1975. Allelopathic potential of western bracken. J. Chem. Ecol. 1: 161-169. Ojika, M., K. Wakamatsu, H.Niwa and K. Yamada. 1987. Ptaquiloside, a potent carcinogen isolated from bracken fern Pteridum aquilinum var. latiusculum: structure elucidation based on chemical and spectral evidence, and reactions with amino acids, nucleosides, and nucleotides Tetrahedron 43: 5261- 5274. Oelrichs, P. B., Jack C. NG., and J. Bartley. 1995. Purification of ptaquiloside, a carcinogen from pteridium aquilinum. Phytochemistry 40: 53-56. Ortega, R. C., A. L. Anaya, and L. Ramos. 1988. Effects of allelopathic compounds of corn pollen on respiration and cell division of watermelon J. Chem. Ecol. 14: 71-86. Yamada, K., M. Ojika, and H. Kigoshic. 2007. Ptaquiloside, the major toxin of bracken, and related terpene glycosides: chemistry, biology and ecology. Nat. Prod. Rep. 24: 798-813.
在花蓮太魯閣山區進行林木復育時,因植物蕨(bracken fern, Pteridium aquilinum (L.) Kuhn)入侵使林木幼苗無法生長。本研究在夏季與冬季蕨類生長區域與非蕨生長區的草相調查中,發現蕨生長區之雜草種類與覆蓋率皆少於非蕨生長區,推測蕨具剋它作用。本研究使用多種蔬菜種子及幼苗作為生物檢定(bioassay)材料,建立生物檢定系統測試蕨植物體各部份水萃液是否具有剋它效果,包含蕨地上部、地下莖、土壤表層枯落葉與其下方土壤。結果發現,蕨地上部與地下莖水萃取液,對於生物檢定材料之種子發芽與胚根伸長的抑制效果最明顯,尤其是地上部水萃液。可知蕨植株的水萃液,應含有剋它物質而影響其他植物生長。
將蕨地上部水萃液經C18 SPE管柱,並利用HPLC系統分離,得到純化之ptaquiloside (PT)與相關化合物,以液相層析串聯式質譜儀與超導核磁共振儀確認其結構。為了瞭解蕨產生剋它作用的原因,接著利用生物檢定材料-白蘿蔔根尖細胞,觀察水萃液中剋它物質如何影響細胞有絲分裂。試驗結果發現PT並非蕨葉水萃液中抑制細胞有絲分裂之成分,推測蕨水萃液中含有其他剋它物質,會抑制植物根尖細胞的有絲分裂。

Due to the invasion of bracken (bracken fern, Pteridium aquilinum (L.) Kuhn) into the Taroko national park, forest recovery is difficult to proceed. In summer and winter, field investigation found that the coverage and species of weeds in the bracken-grown area were decreased, revealing the allelopathy phenomenon. Base on the bioassay system using several vegetables as materials, the allelopathic effect of water extract collected from different parts of fern plant was confirmed. It was obrious that the maximal allelopathic effect existed in water extract from fern frond and rhizome, as compared with existed litter and soil. After C18 SPE extraction procedure and subsequent separation of HPLC system, purified ptaquiloside (PT) and related componds were further confirmed by LC-MS/MS (liquid chomatography -tandem mass spectrometry) and NMR (Nuclear Magnetic Resonance) technique. For the allelopathic mechanism of water extract, mitosis retadation of radish root-tip cell was observed after treatment of water extract of fern frond, whereas the purified PT failed to affect cell mitosis, thus it is suggested that some unknown compounds in water extract might be an effective integradient responsible for allelopathic effect through retardation of all mitosis.
其他識別: U0005-2107200923100600
Appears in Collections:農藝學系

Show full item record

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.