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標題: 以微球體包覆神經幹細胞對週邊神經修復的影響
Neural stem cells cultured in alginate microspheres for peripheral nerve system regeneration
作者: 何佑良
Ho, Yu-Liang
關鍵字: alginate;褐藻膠;microsphere;neural stem cell;chitosan;nano particle;neutrophic factor;control release;flat asymmetrical porous poly-lactide conduits;微球體;神經幹細胞;幾丁聚醣;奈米微粒;神經營養因子;釋放控制;聚乳酸導管
出版社: 化學工程學系所
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The biodegradable material-alginate had been fabricated into microspheres by spray process. Neural stem cells had been encapsulated in the microspheres avoiding the immune response. At the same time, the cell feature including proliferation, gene expression and differentiation had been observed. The cells cultured in microspheres for 3 days, and then the cell density had been increased 4 times to the original density. Based on the same gene expression of BDNF, NGF and GDNF between microspheres cultured cells and TCPS cultured cells. Suggested microspheres don't affect cell biofunctions. Furthermore, the emulsion process had made the nanoparticles by crosslinking reaction between chitosan, alginate and hydroxybutyl chitosan for releasing the neurotrophic factor. To make sure the temperature effect to protein release, the results showed that higher protein adsorbability at 25℃; after 48 hours, 40% protein had released and reach stable releasing rate at 37℃. In the sciatic nerve repair animal model, evaluate the regeneration by walking track analysis and histology. From the above results, the performing filled with microspheres is better than filled with neural stem cells and nerve conduit only. The conclusion is that neural stem cells encapsulated microspheres present the better repair effects in the above animal model.
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