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標題: 水稻抗白葉枯病生理及遺傳之研究
Physiological and Genetical Studies on Bacterial Blight (Xanthomonas oryzae pv. oryzae) Resistance in Rice (Oryza sativa L.)
作者: 張素貞
Chang, Su-Jein
關鍵字: Rice;水稻;Bacterial Blight;Physiological;Genetical;白葉枯病;生理;遺傳
出版社: 農藝學系
白葉枯病之反應情形,結果發現施用高濃度矽肥(150 ppm) 可使感病
率均在80 %以上,無母性效應的現象存在。由秈型稻與梗型稻之世代

The thesis explored mainly the effect of nutrients on the
biochemical and physiological characteristics associated with,
and the genetic studies of bacterial blight resistance in rice
(Oryza sativa L.). The rice materials included susceptible
variety such as TN1 TCS10, TC189, and TNG67, and resistant
variety (strain) such as TCSW1, TSWY1157, TSWY7, and TKY6287.
The clipping inoculation methods of Kauffman et al. (1973) was
adopted to evaluate the resistance performance of test rices
using the XM42 and XF13 strains of Xanthomonas oryzae pv.
oryzae as challenging pathogen. The results obtained are
summsrized as follows :
1. Under the hydroponic culture system, the application of
silicate at 150 ppm, on the contrarily, greatly enhanced
the resistance of both TSWY7 and TN1 plants. An adjunct
chemical analysis during the investigation revealed that
the enhansive effect of silicate application was clearly
associated with the increases of silicon and the decrease
of soluble sugar contents among tested rices.
2. In order to explore the physiological characteristics
associated with bacterial blight resistance, seedlings of
both TSWY7 and TN1 plants were grown by hydroponic culture
in a growth chamber as a model system for the resistance
evaluation. Artificial inoculation of TSWY7 plants with the
XF13 strain of this pathogen generally led to an enhanced
rate of ethylene production throughout a four days
experimental period. Where plant, the enhancement of
pathogenesis related ethylene production was not observed.
In accompany with the increased ethylene production,
continued increase of peroxidase activity was detected from
the soluble protein contents of the XF13 pathogen
inoculated TSWY7 plants for 4 days after inoculation. And
likewise, the enhanced peroxidase activity was not observed
in the compared TN1 plants.
3. In order to explore the genetic behavior of bacterial
blight resistance among the Indica and Japonica rices, two
sets of diallel crosses were undertaken in which 5 Indica
rices (namely TN1, TCS10, TCSW1, TSWY7 and TSWY1157) and 4
Japonica rices (namely TC189, TNG67, TN5 and TKY6287) were
used. The disease resistance performance of their F1
progenies were evaluated by challenge inoculation with the
XF13 and XM42 strains of X. oryzae pv. oryzae by the
clipping method in both greenhouse and field trials. In
addition to the diallel crosses, backcrosses were also
conducted among two sets of Indica rice crosses
(TN1TSWY7, TN1TSWY1157) and two sets of Japonica rice
crosses (TKY6287TC189, TKY6287TNG67). The P1, P2, F1,
F2, BC1 and BC2 backcrosses progenies were likewise
evaluated for their bacterial blight resistance both in
greenhouse and field trials as stated above. The resistance
performance of all tested rice plants were determined by
lesion lengths developed 14 days (for greenhouse trial) or
21 days (for field trial) after artificial inoculation.
Statistic analysis of the results obtained from both the
diallel crosses trial and the backcrosses trial generally
indicated that the resistance of tested rice strains were
characteristic of incomplete dominance. The additive and
dominance effects both appeared to be significant for the
two types of rice varieties tested; and among them, the
additive effect was evidently greater than the dominance
effect. The heritability of the resistance characteristic
was over 80 % in all diallel crosses tested; the maternal
effect was clearly not involved. The data obtained from
generation mean analysis indicated that the continuous
distribution of disease resistance among the F2 progenies of
both types test rices were positively skewed. Morever, the
disease resistance among the F2 appeared to be confered by
genes in a multiplicative way in which the additive and
dominance effect were of great importance.
Appears in Collections:農藝學系

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