Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/37169
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dc.contributor.advisor王慶裕zh_TW
dc.contributor.advisorChing-Yuh Wangen_US
dc.contributor.author蔡謹如zh_TW
dc.date2004zh_TW
dc.date.accessioned2014-06-06T07:58:45Z-
dc.date.available2014-06-06T07:58:45Z-
dc.identifier.urihttp://hdl.handle.net/11455/37169-
dc.description.abstract本論文主要探討水稻突變品系幼苗對固殺草(glufosinate)之耐感反應及其耐感性差異原因。根據水稻台農67號(TNG 67) V4 stage幼苗之株高、地上部鮮重及乾重,以及外觀傷害指數等性狀,經劑量反應分析,選定0.15、0.30及0.75 mM三個濃度進行水稻突變品系葉片片段的耐感性篩選,並配合富士光(FSK)品種一起進行篩選。之後進一步進行幼苗篩選,選出感性品系為FSK及FSK-3,耐性品系為R11-2及R11-3。 根據銨累積分析中顯示耐性品系累積較少的量,進一步分析GS比活性,發現耐性品系受固殺草抑制的程度較感性低。另外,施用固殺草確實造成耐感品系在glyoxylate量上的累積,但是耐感之間並無顯著差異。在固殺草轉運方面,耐感品系在處理14C-glufosinate後,其藥劑大部分殘留在原處理葉位,而耐性品系傾向將藥劑轉運至處理葉位的葉尖累積,而感性品系則轉運至處理葉位的葉尖、底部及葉鞘。另外,亦發現耐性品系的代謝能力大於感性品系。推測耐性水稻突變體對固殺草產生耐性的原因包括轉運受到限制、代謝能力增加及目標酵素GS對該藥劑較不敏感所致。zh_TW
dc.description.abstractThis experiment was conducted to examine the abilities of rice mutants to tolerate glufosinate, and to identify the physiological mechanism of glufosinate tolerance. According to the does-response to glufosinate for the rice cv. Tainung 67 (TNG 67), based on plant height, dry matter production, and injury index, three dosages, i.e. 0.15, 0.30, and 0.75 mM, were used to screen glufosinate-tolerant and -susceptible rice lines. After a series of screening process, rice lines FSK and its progeny FSK-3 were referred to susceptible lines, mutant R11-2 and R11-3 were referred to tolerant lines. Ammonium analysis showed that a less accumulation was found in tolerant rice mutants. In addition, its specific activity of glutamine synthetase was only slightly affected, too. Physiological study using 14C-glufosinate revealed that both the restricted translocation and active metabolism of glufosinate might be partially accounted for the glufosinate tolerance of selected rice mutant.en_US
dc.description.tableofcontents頁次 目錄……………………………………………………………………….i 圖目錄…………………………………………………………………… ii 表目錄………………………………………………………………..….iv 壹、緒言…………………………………………...…………...……...…1 貳、前人研究………………………………………..…………...………7 參、材料與方法……………………………..………………...…...…..16 肆、結果與討論…………………………………….……………...…..29 一、水稻幼苗對固殺草除草劑之劑量反應….……………………..29 二、固殺草耐感性水稻突變品系之篩選…………………..…….…34 三、水稻耐感品系對固殺草耐性機制之生理研究…………….…59 伍、中文摘要…………………………………………………..…...….77 陸、英文摘要………………………………………………………..….78 柒、參考文獻……………………………………………………………79 附錄……………………………………………………………………..87 圖目錄 頁次 圖1. 水稻台農67號幼苗在夏季及冬季處理不同濃度固殺草後第21天株高之生長變化-------------------------------------------------------------------------------------------32 Fig. 1. Plant height of rice cv. TNG 67 as affected by glufosinate at various dosages in both summer (A) and winter (B) seasons.---------------32 圖2. 水稻葉片片段在施用固殺草後出現褪綠(A)、枯黃(B)、壞疽(C)以及褐化(D)等傷害症狀-------------------------------------------------------------------------------------38 Fig. 2. Herbicidal injury for the leaf segment of rice caused by glufosinate. It’s symptoms include chlorosis (A), yellowing (B), necrosis (C) and browning (D).--------------------------------------------------------------------------38 圖 3. 水稻耐感性品系V4 stage幼苗以0.54 mM固殺草劑量噴施後之傷害指數-------------------------------------------------------------------------------------------------56 Fig. 3. Injury index of seedlings for the rice lines at 4-leaf stage after foliar-spraying of glufosinate at 0.54 mM.------------------------------------------56 圖 4. 水稻耐感品系幼苗在夏季和冬季噴施0.26 mM固殺草後之銨累積變化----------------------------------------------------------------------------------------------------60 Fig. 4. Ammonium accumulation in seedlings of rice lines at 4-leaf stage after foliar-spraying of glufosinate at 0.26 mM in both summer (A) and winter (B) seasons.--------------------------------------------------------------------------60 圖 5. 水稻耐感品系幼苗噴施1.02 mM固殺草後之GS比活性變化------------63 Fig. 5. Specific activity of glutamine synthetase in seedlings of rice lines at 4-leaf stage after foliar-spraying of glufosinate at 1.02 mM.-----------63 圖 6. 水稻耐感品系幼苗噴施1.02 mM固殺草後之glyoxylate含量變化------66 Fig. 6. Glyoxylate content in seedlings of three rice lines at 4-leaf stage after foliar-spraying of glufosinate at 1.02 mM.----------------------------------66 圖7. 水稻感性品系FSK-3 於14C-glufosinate處理後24小時的植體外觀傷害(A)及14C-glufosinate處理後24小時經自動放射顯影技術顯示14C-同位素在植體內之分布(B).------------------------------------------------------------------------------------69 Fig. 7. Autoradiography of 14C-glufosinate-treated seedling (A) of rice line FSK-3 24 h after treatment. 14C-Isotope distribution was shown on X-film (B).---------------------------------------------------------------------------------------69 圖8. 水稻感性品系R11-2於14C-glufosinate處理後24小時的植體外觀傷害(A)及14C-glufosinate處理後24小時經自動放射顯影技術顯示14C-同位素在植體內之分布(B).------------------------------------------------------------------------------------70 Fig. 8. Autoradiography of 14C-glufosinate-treated seedling (A) of rice line R11-2 24 h after treatment. 14C-Isotope distribution was shown on X-film (B).---------------------------------------------------------------------------------------70 圖9. 水稻感性品系TNG 67 於14C-glufosinate處理後24小時的植體外觀傷害(A)及14C-glufosinate處理後24小時經自動放射顯影技術顯示14C-同位素在植體內之分布(B).---------------------------------------------------------------------------------71 Fig. 9. Autoradiography of 14C-glufosinate-treated seedling (A) of rice line TNG 67 24 h after treatment. 14C-Isotope distribution was shown on X-film (B).---------------------------------------------------------------------------------------71 圖10. 利用TLC分離技術及自動放射顯影術偵測水稻耐性品系R11-2 (編號1,2)及R11-3 (編號3,4),感性品系FSK (編號5,6)及FSK-3 (編號7,8),以及對照組TNG 67 (編號9,10),經固殺草處理後24小時其植體內固殺草(Glu, Rf 0.16)及其代謝物(MPP, Rf 0.55)的變化------------------------------------------------------------74 Fig. 10. Autoradiography, coupled with TLC separation, of the 14C-glufosinate (Glu, Rf 0.16) and its metabolite (MPP, Rf 0.55) extracted from 14C-glufosinate treated rice for the tolerant lines R11-2 (1,2) and R11-3 (3,4), susceptible lines FSK (5,6) and FSK-3 (7,8), and control line TNG 67 (9,10), respectively, 24 h after treatment.------------------------------74 圖11. 水稻耐感品系處理14C-glufosinate後24小時之代謝變化---------------75 Fig. 11. Metabolism of 14C-glufosinate in leaves of 14C-glufosinate-treated rice lines at V4 stage. Metabolism percentage is the relative percentage of glufosinate metabolite, MPP, produced from 14C-glufosinate.------------------------------------------------------------------------------75 表目錄 頁次 表1. 水稻台農67號四葉期幼苗於夏季和冬季葉面噴施固殺草後之劑量反應--30 Table 1. Dose-response to glufosinate for the seedling growth of rice cv. TNG 67 at 4-leaf stage in both summer and winter.----------------------------30 表2. 水稻突變品系於收穫期其成熟植株葉片片段處理固殺草後之傷害指數---35 Table 2. Injury index of leaf segments for the rice mutants at harvest stage as affected by glufosinate.-----------------------------------------------------35 表3. 水稻突變品系於收穫期成熟植株葉片片段處理固殺草後之葉綠素變化---40 Table 3. Changes of chlorophyll in leaf segments of rice mutants at harvest stage as affected by glufosinate.-----------------------------------------40 表4. 水稻突變品系分蘗期植株葉片片段處理固殺草後之傷害指數---------------43 Table 4. Changes in injury index of leaf segments for the mutant lines of rice at tillering stage.----------------------------------------------------------------------43 表5. 水稻突變品系分蘗期植株葉片片段處理固殺草後之傷害指數與葉綠素變化----------------------------------------------------------------------------------------------------50 Table 5. Changes in both injury index and chlorophyll of leaf segments for the rice lines at tillering stage.-----------------------------------------------------50 表6. 水稻突變品系孕穗期植株葉片片段處理固殺草後之傷害指數與葉綠素變化----------------------------------------------------------------------------------------------------52 Table 6. Herbicidal injury and chlorophyll content of leaf segments for the rice lines at booting stage.---------------------------------------------------------52 表7. 耐感水稻突變品系孕穗期植株葉片片段處理不同濃度固殺草後之傷害指數及葉綠素含量變化----------------------------------------------------------------------------54 Table 7. Effect of glufosinate on both injury index and chlorophyll of leaf segments of rice plants at booting stage.-----------------------------------------54 表8. 水稻耐感性突變品系六葉期幼苗在處理不同濃度固殺草後之傷害指數---58 Table 8. Effect of glufosinate at various dosages on the injury index of rice plants at 6-leaf stage.---------------------------------------------------------------58zh_TW
dc.language.isoen_USzh_TW
dc.publisher農藝學系zh_TW
dc.subjectglufosinateen_US
dc.subject固殺草zh_TW
dc.title水稻突變品系對固殺草(glufosinate)除草劑的耐性機制zh_TW
dc.titleMechanism of glufosinate tolerance for the rice mutantsen_US
dc.typeThesis and Dissertationzh_TW
item.languageiso639-1en_US-
item.openairetypeThesis and Dissertation-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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