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Effects of Aging on Antioxidation of Primed Bitter Gourd Seeds
Yeh, Yung Ming
The poor seed germination can be improved by priming. However, priming is economically adoptable only if the primed seeds can be safely stored for a longer period. The present study was designed to evaluate the storability of primed bitter gourd seeds. Several physiological and biochemical characteristics within primed seeds were also investigated. The results indicated that both accelerated aging and natural aging decreased the levels of antioxidant in treated seeds, however, the extents of changes varied among the aging treatments. Aging also reduced the activities of SOD, CAT, GPX and GR, and decreased the levels of GSH and ASC. SDS-PAGE analysis showed the different expression patterns in Cu/Zn SOD and CAT isoforms on the artificially aged seeds. Significant differences in soluble proteins were also observed on two-dimensional electrophoresis. Aging decreased total antioxidation ability, increased MDA and total peroxides, and reduced seed vigor. Partial vacuum- packing the primed seeds could maintain their viability and vigor for up to 9 months. However, rapid deterioration was observed on primed seeds stored under hermetic condition. It appeared that partial vacuum packing was an effective technique to slow down the deteriorative changes in antioxidation systems of primed seeds and subsequently maintain their vigor and viability.
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