Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/37195
標題: 紫背脈葉蘭種子發育與發芽之研究
Studies on seed development and germination of Nervilia plicata (Andr.) Schlter
作者: 林珈芝
Lin, Chia-Chih
關鍵字: Orchidaceae;蘭科;Nervilia;aseptic seed germination;seed development;seed germination;脈葉蘭;無菌播種;種子發育;種子發芽
出版社: 農藝學系
摘要: 
紫背脈葉蘭為本省珍貴之藥材,藥效與金線連相似,因民間濫採且未加以復育,已日漸稀少。本研究調查蒴果生長曲線,發現初期生長發育迅速,之後生長膨大速率漸漸減緩,大致呈拋物線。
利用紫背脈葉蘭進行無菌播種,試驗的處理及結果如下:
1.以不同成熟度的蒴果進行發芽試驗,結果以14~16天的蒴果有較佳的發芽率。
2.培養基以pH值為5.7±0.1及6.2±0.1者,種子之發芽率較佳(18.3~18.5%)。
3.培養基添加30 g/l蔗糖,可使種子有較佳的發芽率(12.7%)。
4.在添加不同濃度peptone的處理中,以添加0.5 g/l的peptone對種子的發芽率有顯著之提升效果(52.8%)。
5.在添加不同濃度之BA、NAA處理中,發現BA對種子發芽率無顯著促進的效果,但可使發芽之後的根莖轉綠,而NAA對發芽有顯著的抑制效果。
6.在添加不同濃度椰子水的處理中,以添加5%的椰子水有較佳的發芽率(20.5%)。
7.在添加不同濃度的活性碳處理中,額外添加0.5 g/l、1g/l、2g/l的活性碳有促進發芽的效果(51.9~52.6%),但處理之間無顯著差異。
8.以經冷藏處理的蒴果進行發芽試驗,結果發現冷藏0~14天的種子發芽率並無顯著差異(15.7~16.2 %),但冷藏21天的蒴果種子率顯著下降(2.7%)。
9.將幼苗繼代,結果以添加0.5 g/l的peptone者,幼苗之生長情形最為良好。

Nervilia plicata are important medicine plant in Taiwan. The effect of medicine of them are similar to Anoectochilus formosanus. For reason, people over-harvest and don''t recover them, so they disappear gradually.
The capsules of Nervilia plicata in the growth initial phase were fast than the last phase .The expansion curve was almost the parabolic curve.
The experiments of Nervilia plicata were using by aseptic seed germination. The test and result were shown as following:
1.The experiment was tested by the different mature capsules, the result was the growing 14 ~16 days of capsules had the better germination.
2.The pH value of the media were 5.70.1 and pH 6.20.1 had the best germination (18.3~18.5%).
3.The media supplemented with 30 g/l sucrose had the best germination (12.7%).
4.The media supplemented with 0.5 g/l peptone had the best germination (52.8%).
5.The media supplemented with the different concentrations of BA and NAA, the media with BA could not significantly promote germination, but could promote rhizome to green. The media supplemented with NAA could significantly inhibit germination
6.The media supplemented with 5 % coconut milk had the best germination (20.5%).
7.The media supplemented with 0.5 g/l, 1 g/l, and 2 g/l activated charcoal could significantly promote seed germination (51.9~52.6%), but these media were not significantly different.
8.The experiment of capsules by the different days of cold storage, the cold storage 0~14 days could significantly influence seed germination (15.7~16.2%), but 21 days could significantly reduce seed germination.
9.Seedings of N. plicata were subcultured on the media of the 0.5 g/l peptone concentration had the best growth.
URI: http://hdl.handle.net/11455/37195
Appears in Collections:農藝學系

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