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Studies on the Population Variations of Cynodon dactylon (L.) Pers. in Taiwan
|關鍵字:||Cynodon dactylon (L.) Pers.;百慕達草;Taiwan;Population variations;臺灣地區;族群變異||出版社:||農藝學研究所||摘要:||
為探討台灣地區百慕達草（Cynodon dactylon L. Pers.）之族群變異情形，以為將來種源保存及因應不同目的之育種需要，於1990年11月至1991年3月間赴台灣21個地區蒐集原野地生長之百慕達草，種植於台中縣新社鄉，1991年6月採取第二次＊芽鮮重相當二之節苗，種植於盛裝1公升之95%洗沙與5%腐質土混合之介質的o15cm＊13cm之塑膠盆中60天後進行形態之調查及蘋果酸氫脫酵素葡萄糖6-磷酸脫氣酵素與酯＊之電泳分析；形態部份則估算各族群各種性狀之分佈特性，並以多數變方分析及判別分析進行群間比較；同功異構＊電泳分析部份，則統計其各條帶於各族群中出現之比率，並進行群叢分析。其結果摘要如下：
The purpose of present experiment is to evaluation population variation of Bermude grass in Taiwan for germplasm conservation and breeding program.
A number of wild-type grasses were collected from 21 different locations during Novermber 1990 to March 1991. The plants were cultivated in the nursery at Taiwan seed improvement and propagation station. The stocks were cut into pices with two node and planted into the plastic pot (ψ15*13cm) which contained 1 liter media composed at 5% peatmoss and 95% river sand by volume. After six moths, the agronomic characteristics and morphological traits were investigated. The variations within the populations were also analyzed. The genotypical differences of the grass were identified by using isozyme (6-Phosphogluconate dehydrogenase, Malate dehydrogenase and Esterase) electrophoresis. The results were summrized as follows:
1.All of the agronomic characters showed significantly differences among the 21 populations of grasses.
2.The results from multivariance analysis showed that Hsindien between Pao-shan, Pei-men between Nan-hua, Nan-hua between Yuh-ching, Tai-pao between Ken-ding, Tai-tung between Tung-ho, Fong-lin between Chi-an, Fong-lin between Fu-li, Chi-an between Fu-li gave no significant differences. But, the rest populations significant differences.
3.The discriminant analysis was used for the identification of 21 different populations, all of the grasses could be classified into three groups according to the first and second discriminant function which a centroid was constructed.
4.In the isozyme electrophoresis, there are seven bands included 3rd, 4th, 9th, 10th, 14th, 17th and 18th band were found in all of the populations with electrophoresis of Esterase. As for 6-Phosphogluconate dehydrogenase four identical bands of were found in all populations 1st, 2nd, 3rd and 6th. Also four identical bands of 6th, 9th, 16th and 19th were found in the Malate dehydrogenase electrophoresis.
5.Result from cluster analysis using electrophoresis data showed that Bermuda grass in Taiwan could be classified into a number of related groups included Hsin-dien and Pao-shan, Yana-mei and Luen-bei, Chin-shui and Tai-tung, Hsin-sheh and Fong-lin,Nan-hua and Yuh-ching, Heng-chun and Ken-ding, and other independent ones.
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