Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/37227
標題: 百慕達草在臺灣地區的族群變異研究
Studies on the Population Variations of Cynodon dactylon (L.) Pers. in Taiwan
作者: 陳紫淵
Chen, Tzyy-Iuan
關鍵字: Cynodon dactylon (L.) Pers.;百慕達草;Taiwan;Population variations;臺灣地區;族群變異
出版社: 農藝學研究所
摘要: 
為探討台灣地區百慕達草(Cynodon dactylon L. Pers.)之族群變異情形,以為將來種源保存及因應不同目的之育種需要,於1990年11月至1991年3月間赴台灣21個地區蒐集原野地生長之百慕達草,種植於台中縣新社鄉,1991年6月採取第二次*芽鮮重相當二之節苗,種植於盛裝1公升之95%洗沙與5%腐質土混合之介質的o15cm*13cm之塑膠盆中60天後進行形態之調查及蘋果酸氫脫酵素葡萄糖6-磷酸脫氣酵素與酯*之電泳分析;形態部份則估算各族群各種性狀之分佈特性,並以多數變方分析及判別分析進行群間比較;同功異構*電泳分析部份,則統計其各條帶於各族群中出現之比率,並進行群叢分析。其結果摘要如下:
1.台灣地區參試的21個百慕達草族群各性狀之平均值與相關均具有顯著性差異存在。
2.以多變數變方分析統計,結果顯示21個族群間除了新店與寶山、北門與南化、南化與玉井、太保與墾丁、台東與東河、鳳林與吉安、鳳林與富里、吉安與富里等族群間無顯著差異外,其他各族群間均具顯著性差異。
3.在判別分析中,由第一判別函數與第二判別函數及第一與第三判別函數所構成的形心位置中發現,21個百慕達草族群約略可以分成三群。
4.由同功異構*電泳分析中可發現,酯*之第3、4、9、10、14、17、18等條帶為各族群所共有;葡萄糖6-磷酸況氫酵素之第1、2、3、6等四條帶為各族群所共有;蘋果酸氫酵素之第6、9、16、19等四條帶為各族群所共有。
5.由電泳分析所得資料進行群叢分析得知:新店與寶山、楊梅與崙背、清水與台東、新社與鳳林、南化與玉井、恆春與墾丁等族群各成一群,其他各族群則各成獨立群。

The purpose of present experiment is to evaluation population variation of Bermude grass in Taiwan for germplasm conservation and breeding program.
A number of wild-type grasses were collected from 21 different locations during Novermber 1990 to March 1991. The plants were cultivated in the nursery at Taiwan seed improvement and propagation station. The stocks were cut into pices with two node and planted into the plastic pot (ψ15*13cm) which contained 1 liter media composed at 5% peatmoss and 95% river sand by volume. After six moths, the agronomic characteristics and morphological traits were investigated. The variations within the populations were also analyzed. The genotypical differences of the grass were identified by using isozyme (6-Phosphogluconate dehydrogenase, Malate dehydrogenase and Esterase) electrophoresis. The results were summrized as follows:
1.All of the agronomic characters showed significantly differences among the 21 populations of grasses.
2.The results from multivariance analysis showed that Hsindien between Pao-shan, Pei-men between Nan-hua, Nan-hua between Yuh-ching, Tai-pao between Ken-ding, Tai-tung between Tung-ho, Fong-lin between Chi-an, Fong-lin between Fu-li, Chi-an between Fu-li gave no significant differences. But, the rest populations significant differences.
3.The discriminant analysis was used for the identification of 21 different populations, all of the grasses could be classified into three groups according to the first and second discriminant function which a centroid was constructed.
4.In the isozyme electrophoresis, there are seven bands included 3rd, 4th, 9th, 10th, 14th, 17th and 18th band were found in all of the populations with electrophoresis of Esterase. As for 6-Phosphogluconate dehydrogenase four identical bands of were found in all populations 1st, 2nd, 3rd and 6th. Also four identical bands of 6th, 9th, 16th and 19th were found in the Malate dehydrogenase electrophoresis.
5.Result from cluster analysis using electrophoresis data showed that Bermuda grass in Taiwan could be classified into a number of related groups included Hsin-dien and Pao-shan, Yana-mei and Luen-bei, Chin-shui and Tai-tung, Hsin-sheh and Fong-lin,Nan-hua and Yuh-ching, Heng-chun and Ken-ding, and other independent ones.
URI: http://hdl.handle.net/11455/37227
Appears in Collections:農藝學系

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