Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3727
標題: 製備多層壁奈米碳管奈米複合材料作為尼古丁與尿素感測器之探討
Preparation of multiwalled carbon nanotube nanocomposites for nicotine and urea sensors
作者: 王尚任
Wang, Shang-Jen
關鍵字: Multiwalled carbon nanotube;多層壁奈米碳管;Nicotine;Urea;Sensor;尼古丁;尿素;感測器
出版社: 化學工程學系所
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摘要: 
本論文第一部分中以多層壁奈米碳管(multiwalled carbon nanotubes, MWNTs)-三氧化二鋁包覆二氧化矽奈米顆粒(alumina-coated silica, ACS)複合材料修飾玻璃碳電極(glassy carbon electrode, GCE)電催化氧化尼古丁,經由場發式電子顯微鏡的微觀表面形貌分析,可以確定帶正電荷之ACS奈米顆粒確實可圍繞於MWNTs之上,且可有效的將MWNTs彼此間分散開來。由結果顯示出MWNTs可催化尼古丁物質之電化學反應,MWNTs-ACS奈米複合材料修飾電極對於尼古丁的催化表現是由循環伏安法及安培法所證明,經由選擇出最佳的pH 8.0值及最佳測量電位0.7 V作為測量尼古丁濃度,其MWNTs-ACS奈米複合材料修飾GCE可有效降低電化學氧化電位,並防止電極表面毒化以及提高電流訊號。以最適值所製備出的MWNTs-ACS奈米複合材料薄膜對於偵測nicotine具其靈敏度達1.786 A M-1cm-2,線性範圍達690 μM、偵測極限達1.42 μM。
本論文第二部份以利用最適當的MWNTs-幾丁聚醣(Chitosan)複合材料基質圈入尿素酶(Urease, Urs)修飾於GCE上作為電流式尿素生物感測器,經由原子力顯微鏡(atomic force microscope, AFM)觀察其表面形貌,進而了解Urs在MWNTs-Chitosan薄膜中扮演的角色,並得知Urs能吸附於奈米碳管之上,且Urs均勻的分散在薄膜表面。MWNTs-Chitosan-Urs奈米生物複合薄膜修飾電極偵測尿素(Urea)。此複合薄膜中奈米碳管展現出優勢的偵測表現是由循環伏安法及安培法所證明。為了獲得本感測器之最佳化條件,經由探討幾個實驗條件的最適值(如操作電壓、緩衝溶液之pH值及酵素承載量)。本MWNTs-Chitosan-Urs奈米生物複合薄膜修飾GCE之尿素生物感測器其線性範圍從30到610 μM,而靈敏度為20.4 A M-1cm-2,應答時間約25秒。

Electrocatalytic oxidation of nicotine at multiwalled carbon nanotube (MWCNT)-alumina-coated silica (ACS) nanocomposite modified glassy carbon electrode are described. The dispersed MWNTs was characterized by transmission electron microscopy. The sensing performance of the MWCNT-ACS nanocomposite modified glassy carbon electrode for the electrooxidation of nicotine was investigated using cyclic voltammetry and amperometry in 0.1 M phosphate buffer solution (pH 8). The MWCNT-ACS nanocomposite modified glassy carbon electrode exhibited the abilities to decrease the electrooxidation potential, to prevent the electrode surface fouling, and to raise the current responses. The MWCNT-ACS nanocomposite responded rapidly to nicotine with a sensitivity of 1.786 A M-1cm-2, a linear range up to 690 μM and a detection limit of 1.42 μM.
A composite of multiwalled carbon nanotubes-chitosan (MWNTs-Chitosan) was used as a matrix for entrapment of urease (Urs) onto a glassy carbon electrode in order to fabricate amperometric biosensor. The homogeneity of the resulting nanobiocomposite film was characterized by atomic force microscopy (AFM). The performance of the MWNTs-Chitosan-Urs nanobiocomposite modified glassy carbon electrode was examined using cyclic voltammetry and amperometry in presence of Urea. The influence of several experimental parameters such as enzyme loading, solution pH value , applied potential was explored to optimize the electroanalytical performance of the biosensor. The optimized biosensor on MWNTs-Chitosan-Urs modified GCE shows a linear current response to the urea concentration ranging from 30 to 610 μM and a sensitivity of 20.4 mA M-1cm-2 with a response time of about 25 s.
URI: http://hdl.handle.net/11455/3727
其他識別: U0005-1007200900174200
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