Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3729
DC FieldValueLanguage
dc.contributor邱信程zh_TW
dc.contributorHsin-Cheng Chiuen_US
dc.contributor廖建勛zh_TW
dc.contributorChien-Shiun Liaoen_US
dc.contributor.advisor蔡毓楨zh_TW
dc.contributor.advisorYu-Chen Tsaien_US
dc.contributor.author李振安zh_TW
dc.contributor.authorLee, Chen-Anen_US
dc.contributor.other中興大學zh_TW
dc.date2010zh_TW
dc.date.accessioned2014-06-06T05:32:38Z-
dc.date.available2014-06-06T05:32:38Z-
dc.identifierU0005-1007200914040900zh_TW
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dc.identifier.urihttp://hdl.handle.net/11455/3729-
dc.description.abstract摘要 本研究成功的利用奈米生物複合薄膜製備出安培法生物感測器偵測乙醇,奈米生物複合薄膜由多層壁奈米碳管(multiwalled carbon nanotubes, MWNTs)、幾丁聚醣(chitosan, CHIT)、酒精去氫酶(alcohol dehydrogenase, ADH)組成並塗佈在玻璃碳電極(glassy carbon electrode, GCE)上。此MWNTs-CHIT-ADH奈米生物複合薄膜之分散均勻與否在本研究中以場發射電子顯微鏡(field emission scanning electron microscopy,FESEM)觀察並作探討。在此研究中使用循環伏安法和安培法作為MWNTs-CHIT-ADH 奈米生物複合薄膜偵測乙醇之催化活性研究。在此乙醇感測研究中針對一系列之重要參數做最適化研究,這些重要參數包括了工作電位、溶液之pH值、輔酶(NAD+)濃度、酵素負載量等。此最適化生物感測器經整理後可得到ADH最適量為10 mg ml-1,靈敏度0.1646 A M-1cm-2,Michaelis-Menten 常數0.38 mM,偵測極限0.52 µM。最後為了能將此乙醇生物感測器應用在市售酒精性飲料,因此針對台灣啤酒、紅葡萄酒及高粱酒等作檢測,發現與商用標示相當接近,表示此酒精生物感測器可精確得到市 售酒精濃度。 在兒茶素感測研究中,採用化學感測器做測量,且以方波伏安法進行測試,發現以MWNTs-ACS修飾GCE之工作電極在對兒茶素偵測時可以得到很高的靈敏度。可得效能參數分別為靈敏度245.7778 A M-1 cm-2,偵測極限0.141µM。接著針對市售茶類飲料所含兒茶素含量做偵測,發現偵測值在商標所標示之物差範圍內,表示此化學感測 器可準確得知兒茶素含量。zh_TW
dc.description.abstractAbstract This study includes the ethanol biosensors and the catechin chemical sensors. The first part is the determination of ethanol by using multiwalled carbon nanotubes (MWNTs), a chitosan (CHIT) binder, and an alcohol dehydrogenase (ADH) function to form a nanbiocomposite film. The MWNTs-CHIT-ADH nanobiocomposite film is cast on a glassy carbon electrode. Homogeneity of the resulting nanobiocomposite film was investigated by field emission scanning electron microscopy(FE-SEM). Electrocatalytic activity of the MWNTs-CHIT-ADH nanobiocomposite was determined using cyclic voltammetry and amperometry. Optimum conditions of the biosensor were experimentally determined through varying several important parameters. These included working potential, solution pH value, NAD+ concentration, and enzyme loading. The optimized biosensor shows a sensitivity of 0.1646 A M-1 cm-2, an apparent Michaelis–Menten constant of 0.38 mM, and a detection limit of 0.52 μM. The applicability of the proposed biosensor based on MWNTs-CHIT-ADH nanobiocomposite was tested by the detection of ethanol in beer, red wine, and spirit. The second part of determination of catechin has been performed at this study. The MWNTs are dispersed into ACS aqueous solution by ultrasonic and adsorption on a glassy carbon electrode. The MWNTs-ACS nanocomposite was characterized by SEM. The sensitivity of the catechin sensor is much higher by using the MWNTs-ACS modified glassy carbon electrode. The chemical sensor of catechin displays a sensitivity of 246. 7778 A M-1 cm-2 and a detection limit of 0.141 µM 。zh_TW
dc.description.tableofcontents總目錄 摘要 I Abstract II 表目錄 V 圖目錄 VI 第一章 緒論 1 1-1 前言 1 1-2 分析物簡介 2 1-2-1 乙醇 2 1-2-2兒茶素 3 1-3文獻回顧 7 1-3-1乙醇 7 1-3-2兒茶素 9 1-4 生物感測器 12 1-4-1 基本構造與組成 12 1-4-2 酵素簡介 15 1-4-3 酵素動力學 15 1-5 奈米碳管 19 1-5-1 奈米碳管的性質 19 1-5-2 奈米碳管的應用 22 1-6幾丁聚醣 22 1-6-1 幾丁聚醣的特性 22 1-6-2 幾丁聚醣的應用 24 1-6-3 幾丁聚醣在感測器上的應用 24 1-7 三氧化二鋁包覆二氧化矽奈米顆粒 25 1-8 自組裝奈米薄膜 26 1-9 電化學方法 27 1-9-1 循環伏安法 27 1-9-2 安培法 29 1-9-3 方波伏安法 31 第二章 實驗方法與步驟 33 2-1 實驗藥品 33 2-2 實驗儀器 33 2-3 實驗步驟 34 2-3-1電極前處理 34 2-3-2 MWNTs-Chitosan-ADH生物複合薄膜修飾於玻璃碳電極(GCE) 35 2-3-3以自組裝的方式將MWNTs-ACS複合材料修飾於GCE 35 第三章 結果與討論 37 3-1 MWNTs-CHIT-ADH 奈米生物複合薄膜修飾GCE 37 3-1-1以FE-SEM 觀察MWNTs-CHIT-ADH 奈米生物複合薄膜 37 3-1-2 MWNTs-CHIT-ADH 奈米生物複合薄膜修飾GCE偵測乙醇 39 3-2 MWNTs-ACS 奈米複合薄膜以自組裝方式吸附於GCE 56 3-2-1以FE-SEM 觀察MWNTs-ACS 奈米複合薄膜 56 3-2-2 MWNTs-ACS 奈米複合薄膜以循環伏安法偵測兒茶素( Catechin ) 58 第四章 結論及未來展望 68 4-1結論 68 4-2 未來展望 68 第五章 參考文獻 70zh_TW
dc.language.isoen_USzh_TW
dc.publisher化學工程學系所zh_TW
dc.relation.urihttp://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1007200914040900en_US
dc.subjectcarbon nanotubeen_US
dc.subject乙醇去氫酶乙醇zh_TW
dc.subjectalcohol dehydrogenaseen_US
dc.subjectethanolen_US
dc.subjectcatechinen_US
dc.subject兒茶素zh_TW
dc.title製備多層壁奈米碳管奈米複合材料做為乙醇和兒茶素感測器之探討zh_TW
dc.titlePreparation of multiwalled carbon nanotube nanocomposites for ethanol and catechin sensorsen_US
dc.typeThesis and Dissertationzh_TW
item.openairetypeThesis and Dissertation-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
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